##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632241.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1784543
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23107540698095	33.0	31.0	34.0	30.0	34.0
2	32.42565127318311	34.0	31.0	34.0	31.0	34.0
3	32.504850821751006	34.0	31.0	34.0	31.0	34.0
4	36.07551345078264	37.0	35.0	37.0	35.0	37.0
5	35.80197283001866	37.0	35.0	37.0	35.0	37.0
6	35.81610978272869	37.0	35.0	37.0	35.0	37.0
7	35.79031998668567	37.0	35.0	37.0	35.0	37.0
8	35.770983383420855	37.0	35.0	37.0	35.0	37.0
9	37.48489837454183	39.0	37.0	39.0	35.0	39.0
10	37.384154934904906	39.0	37.0	39.0	35.0	39.0
11	37.466623107428624	39.0	37.0	39.0	35.0	39.0
12	37.41849762095954	39.0	37.0	39.0	35.0	39.0
13	37.45613022493714	39.0	37.0	39.0	35.0	39.0
14	38.70013555291187	40.0	38.0	41.0	35.0	41.0
15	38.693542828612145	40.0	38.0	41.0	35.0	41.0
16	38.668584057655096	40.0	38.0	41.0	35.0	41.0
17	38.65130400332186	40.0	38.0	41.0	35.0	41.0
18	38.64447144170804	40.0	38.0	41.0	35.0	41.0
19	38.68304658391532	40.0	38.0	41.0	35.0	41.0
20	38.649730491223806	40.0	38.0	41.0	35.0	41.0
21	38.618485517020325	40.0	38.0	41.0	35.0	41.0
22	38.57714103834987	40.0	38.0	41.0	35.0	41.0
23	38.52665584410126	40.0	38.0	41.0	34.0	41.0
24	38.46628688689485	40.0	38.0	41.0	34.0	41.0
25	38.39804364478749	40.0	38.0	41.0	34.0	41.0
26	38.28602953249095	40.0	38.0	41.0	34.0	41.0
27	38.20030394336253	40.0	38.0	41.0	34.0	41.0
28	38.12604683664109	40.0	38.0	41.0	34.0	41.0
29	38.06071638509131	40.0	38.0	41.0	33.0	41.0
30	37.974328441511354	40.0	37.0	41.0	33.0	41.0
31	37.881166774911	40.0	37.0	41.0	33.0	41.0
32	37.78390041596084	40.0	37.0	41.0	33.0	41.0
33	37.70372919005034	40.0	37.0	41.0	33.0	41.0
34	37.624877069367336	40.0	37.0	41.0	33.0	41.0
35	37.59842211703501	39.0	37.0	41.0	33.0	41.0
36	37.55517350940829	39.0	37.0	41.0	33.0	41.0
37	37.48673806122912	39.0	37.0	41.0	33.0	41.0
38	37.426827484683756	39.0	37.0	41.0	32.0	41.0
39	37.36816596742135	39.0	36.0	40.0	32.0	41.0
40	37.28872714190692	39.0	36.0	40.0	32.0	41.0
41	37.23491168327129	39.0	36.0	40.0	32.0	41.0
42	37.174129735175896	39.0	36.0	40.0	32.0	41.0
43	36.24436900651875	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	1.0
12	0.0
13	0.0
14	3.0
15	5.0
16	11.0
17	26.0
18	70.0
19	111.0
20	207.0
21	393.0
22	706.0
23	1311.0
24	2050.0
25	3197.0
26	4855.0
27	7252.0
28	10577.0
29	14816.0
30	20370.0
31	26719.0
32	36063.0
33	49137.0
34	69675.0
35	101668.0
36	165413.0
37	297517.0
38	555464.0
39	416922.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.000332858328434	18.861523650592897	12.668229344991966	26.469914146086705
2	19.80299718191156	20.3435277267065	34.231004800668856	25.622470290713085
3	20.120725586326582	21.514359698813646	29.416550904068995	28.948363810790774
4	16.471556023026622	15.362868812911765	32.845664128014846	35.31991103604676
5	16.602682031197904	34.375579630191034	32.47369214415119	16.54804619445987
6	35.10271257122972	33.47770269475154	15.152058538236398	16.267526195782338
7	30.414789668839585	29.3676308164051	20.30503047558955	19.912549039165768
8	26.726058156065726	33.16653059074508	19.63404636369087	20.47336488949832
9	26.8144841564479	14.144573708787068	19.45506496621264	39.5858771685524
10	18.370865818307543	25.442480231633535	31.13043507497438	25.056218875084546
11	36.7978804657551	21.0917304878616	20.973324823218046	21.137064223165257
12	22.45801866360183	24.535805525560324	27.842758622235497	25.16341718860235
13	30.92887086497776	18.647743427869205	24.0354533345512	26.387932372601835
14	23.443256901066547	19.79083720594012	23.790796859476067	32.97510903351726
15	26.9926250025917	25.920473757146787	21.119188498119687	25.967712742141824
16	26.11335227002095	25.3572483263222	22.813011510509973	25.716387893146873
17	25.10088016932066	25.317742413603934	24.112223689762587	25.469153727312822
18	24.721119076424607	24.21365021745063	25.210375989819244	25.85485471630552
19	25.94781969389362	24.293726741244118	25.141955111196534	24.616498453665727
20	26.260112533012652	23.84778623995051	24.913044964453086	24.979056262583754
21	26.19869624884354	24.369208251076046	23.88364976355291	25.548445736527505
22	26.399531981016988	24.01018075776263	23.96277366250071	25.62751359871967
23	25.81305129660647	24.25534156363842	24.145677632872953	25.785929506882155
24	26.17633758334767	24.108245080112948	24.15615650617553	25.55926083036385
25	26.053617088520703	23.48567672507751	24.481898166645465	25.978808019756315
26	25.597421860947033	24.006370258379878	24.490976121057322	25.905231759615766
27	25.84252663006719	23.905672208515007	24.188657824440206	26.06314333697759
28	25.46097236099102	24.225978303688954	24.5540735078953	25.758975827424724
29	25.403030355670893	24.6164424169101	24.59884687564267	25.381680351776335
30	25.249881902537513	24.909906906137874	24.376380955796524	25.46383023552809
31	25.734543801970588	24.32443488332867	24.368872030542274	25.572149284158463
32	24.820528280910015	24.22900428849291	24.85762461313625	26.092842817460827
33	25.005505611240526	23.84728190914985	25.04030443648598	26.106908043123646
34	25.45626527351821	23.47564614581997	25.15164946992031	25.916439110741518
35	25.19603058037828	23.50736294950584	25.47811960821342	25.818486861902457
36	24.91904089730536	23.358137069266473	25.622078033423684	26.100744000004482
37	25.098862846118024	22.323138192803423	25.963622058980928	26.614376902097625
38	24.119508467994326	22.61144730051335	27.08155533377453	26.18748889771779
39	24.030802283834014	21.92398838245982	27.809696936414536	26.235512397291632
40	23.762106040594148	21.724889789710865	28.5517916912061	25.961212478488893
41	22.91158016366095	22.107508757143986	29.09187394195601	25.889037137239058
42	22.876164934103578	21.81544518680693	29.047100574208635	26.261289304880858
43	22.105715580963867	21.739963676975	29.34544026117611	26.808880480885023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	139.5
2	161.0
3	362.0
4	563.0
5	563.0
6	702.0
7	841.0
8	862.5
9	884.0
10	1216.5
11	1549.0
12	1549.0
13	2608.0
14	3667.0
15	5794.5
16	7922.0
17	7637.5
18	7353.0
19	7353.0
20	8380.0
21	9407.0
22	9556.0
23	9705.0
24	11150.0
25	12595.0
26	12595.0
27	14457.5
28	16320.0
29	21482.0
30	26644.0
31	29852.0
32	33060.0
33	33060.0
34	37850.5
35	42641.0
36	47934.0
37	53227.0
38	62043.5
39	70860.0
40	70860.0
41	79424.5
42	87989.0
43	91454.5
44	94920.0
45	104139.0
46	113358.0
47	113358.0
48	122657.5
49	131957.0
50	143098.0
51	154239.0
52	164538.0
53	174837.0
54	174837.0
55	168621.0
56	162405.0
57	155345.0
58	148285.0
59	135583.5
60	122882.0
61	122882.0
62	110119.5
63	97357.0
64	83497.5
65	69638.0
66	58557.5
67	47477.0
68	47477.0
69	39304.5
70	31132.0
71	25732.0
72	20332.0
73	16293.0
74	12254.0
75	12254.0
76	9622.0
77	6990.0
78	5833.5
79	4677.0
80	3767.5
81	2858.0
82	2858.0
83	2228.0
84	1598.0
85	1269.5
86	941.0
87	738.0
88	535.0
89	535.0
90	404.0
91	273.0
92	174.0
93	75.0
94	44.0
95	13.0
96	13.0
97	8.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1784543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.36971835132294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.16336006268551	59.39540736329397
2	7.177639211446552	9.671110642051318
3	1.8943127548607097	3.8285795029285405
4	0.8200535329451013	2.2098710219007525
5	0.4568151336229496	1.5387753445400039
6	0.273976229105966	1.1074620833895874
7	0.19568731626936317	0.9228379567395275
8	0.14057266578888825	0.7576272721673641
9	0.10135254541854366	0.6145283195133258
>10	0.6508401129670855	8.355342064110639
>50	0.06970646153050306	3.2870214211061892
>100	0.05277552213190349	6.532455774874974
>500	0.002409859588168809	1.0733117728683546
>1k	4.985916389314777E-4	0.7056694605156275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3476	0.19478376256554197	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3007	0.1685025241756573	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2528	0.14166091822948507	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	5.603675562875201E-5	0.0	0.0
4	0.0	0.0	5.603675562875201E-5	0.0	0.0
5	0.0	0.0	5.603675562875201E-5	0.0	0.0
6	0.0	0.0	5.603675562875201E-5	0.0	0.0
7	0.0	0.0	5.603675562875201E-5	0.0	0.0
8	0.0	0.0	5.603675562875201E-5	0.0	0.0
9	0.0	0.0	5.603675562875201E-5	0.0	0.0
10	0.0	0.0	5.603675562875201E-5	0.0	0.0
11	0.0	0.0	5.603675562875201E-5	0.0	0.0
12	0.0	5.603675562875201E-5	5.603675562875201E-5	0.0	0.0
13	0.0	5.603675562875201E-5	5.603675562875201E-5	0.0	0.0
14	0.0	5.603675562875201E-5	5.603675562875201E-5	5.603675562875201E-5	5.603675562875201E-5
15	0.0	5.603675562875201E-5	5.603675562875201E-5	1.6811026688625603E-4	5.603675562875201E-5
16	0.0	5.603675562875201E-5	5.603675562875201E-5	3.3622053377251206E-4	5.603675562875201E-5
17	0.0	5.603675562875201E-5	5.603675562875201E-5	6.164043119162721E-4	5.603675562875201E-5
18	0.0	5.603675562875201E-5	5.603675562875201E-5	6.724410675450241E-4	5.603675562875201E-5
19	0.0	5.603675562875201E-5	5.603675562875201E-5	8.965880900600322E-4	5.603675562875201E-5
20	0.0	5.603675562875201E-5	5.603675562875201E-5	0.0011767718682037921	5.603675562875201E-5
21	0.0	5.603675562875201E-5	1.1207351125750402E-4	0.0014009188907188003	1.1207351125750402E-4
22	0.0	5.603675562875201E-5	1.1207351125750402E-4	0.0021854334695213286	1.1207351125750402E-4
23	0.0	5.603675562875201E-5	1.1207351125750402E-4	0.0030259848039526087	1.1207351125750402E-4
24	0.0	5.603675562875201E-5	1.1207351125750402E-4	0.004651050717186417	1.1207351125750402E-4
25	0.0	5.603675562875201E-5	1.1207351125750402E-4	0.005323491784731441	1.1207351125750402E-4
26	0.0	5.603675562875201E-5	1.1207351125750402E-4	0.00919002792311533	1.1207351125750402E-4
27	0.0	5.603675562875201E-5	1.1207351125750402E-4	0.02869081888192103	1.6811026688625603E-4
28	0.0	1.1207351125750402E-4	1.1207351125750402E-4	0.09980146177480734	1.6811026688625603E-4
29	0.0	1.1207351125750402E-4	1.1207351125750402E-4	0.20094780568470472	1.6811026688625603E-4
30	0.0	1.1207351125750402E-4	1.1207351125750402E-4	0.3329143651904157	1.6811026688625603E-4
31	0.0	1.1207351125750402E-4	1.1207351125750402E-4	0.8081620896778615	1.6811026688625603E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1190	0.0	22.386555	1
TCTTATA	2880	0.0	18.564236	37
CTTATAC	1510	0.0	17.274836	37
TAATACT	150	8.108327E-8	14.799999	4
TCTAATA	125	2.96078E-6	14.799999	2
TTATACT	130	4.446989E-6	14.230768	4
GTATCAA	1890	0.0	13.997354	2
ATTACTC	120	3.3023665E-5	13.875001	3
CGAATTA	95	0.0012455456	13.631579	15
GTGCTAG	315	0.0	13.507937	1
ACTATAC	180	5.1630195E-8	13.361111	3
ATAGGAC	155	1.8889823E-6	13.129032	3
GTATAAA	155	1.8889823E-6	13.129032	1
TACTATA	185	7.389099E-8	13.0	2
ACATACC	145	1.372867E-5	12.75862	8
ACGCGCG	235	8.094503E-10	12.595745	21
CTCTTAT	4425	0.0	12.375141	37
CTAGATT	315	0.0	12.333334	4
GTATATA	195	1.4684701E-7	12.333334	1
GATTATA	105	0.0026445559	12.333333	1
>>END_MODULE