FastQCFastQC Report
Fri 10 Feb 2017
ERR1632240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369489
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT4590.12422561970721725No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC4560.12341368755226813No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA4280.11583565410607623No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA4150.11231728143462998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1800.030.8333321
CTTTTAG551.898131E-523.5454541
GCTTTAT1900.019.4736841
GTATCAA2950.018.8135592
ACTCTTT500.0070260718.58
GGACCTT500.0070260718.56
TCTTATA2650.016.75471737
GAGCAAT904.432834E-516.44444511
TATTAGC803.3747993E-416.18752
GGGTTAG700.00258803215.8571421
TCAGGAC855.3519255E-415.2352943
ATCAACG3650.015.2054794
CTTATAC1853.0195224E-1015.00000137
TCAACGC3750.014.8000015
GTCTTGC750.004097400214.81
TATACTT750.004097400214.85
TTATTGA2006.184564E-1114.84
CAACGCA3800.014.6052646
ACAGGAC908.2552957E-414.3888883
AGAGTAA1051.6508374E-414.09523833
Produced by FastQC (version 0.11.5)