##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632240.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369489
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.912135408631922	31.0	31.0	34.0	30.0	34.0
2	32.11558936801907	33.0	31.0	34.0	30.0	34.0
3	32.20399795393097	34.0	31.0	34.0	30.0	34.0
4	35.86357915932545	37.0	35.0	37.0	35.0	37.0
5	35.39623371737724	37.0	35.0	37.0	33.0	37.0
6	35.374287191228966	37.0	35.0	37.0	33.0	37.0
7	35.36651700050611	37.0	35.0	37.0	33.0	37.0
8	35.3301451464049	37.0	35.0	37.0	32.0	37.0
9	37.01040355734541	39.0	37.0	39.0	33.0	39.0
10	36.83087182568358	38.0	37.0	39.0	33.0	39.0
11	36.9713063176441	39.0	37.0	39.0	33.0	39.0
12	36.89371808091716	39.0	37.0	39.0	33.0	39.0
13	36.95327059804216	39.0	37.0	39.0	33.0	39.0
14	38.09111231998787	40.0	37.0	40.0	33.0	41.0
15	38.05210709926412	40.0	37.0	40.0	33.0	41.0
16	38.02931345723418	39.0	37.0	40.0	33.0	41.0
17	38.01216003724062	39.0	37.0	40.0	33.0	41.0
18	38.00622210674743	39.0	37.0	40.0	33.0	41.0
19	38.02847446067407	40.0	37.0	41.0	34.0	41.0
20	37.995307032144396	40.0	37.0	41.0	33.0	41.0
21	37.9695011218196	39.0	37.0	40.0	33.0	41.0
22	37.92211405481625	39.0	37.0	40.0	33.0	41.0
23	37.87816958014988	39.0	37.0	40.0	33.0	41.0
24	37.8026571832991	39.0	37.0	40.0	33.0	41.0
25	37.724684632018814	39.0	37.0	40.0	33.0	41.0
26	37.60133048615791	39.0	37.0	40.0	33.0	41.0
27	37.504312712963035	39.0	37.0	40.0	32.0	41.0
28	37.42208293075031	39.0	37.0	40.0	32.0	41.0
29	37.33405324651072	39.0	36.0	40.0	32.0	41.0
30	37.23527358053961	39.0	36.0	40.0	32.0	41.0
31	37.136391069828875	39.0	36.0	40.0	31.0	41.0
32	37.03360587189334	39.0	36.0	40.0	31.0	41.0
33	36.93041741432086	39.0	36.0	40.0	31.0	41.0
34	36.881844926371286	39.0	36.0	40.0	31.0	41.0
35	36.85300238978697	39.0	36.0	40.0	31.0	41.0
36	36.80350700562128	39.0	36.0	40.0	31.0	41.0
37	36.74124534153926	39.0	35.0	40.0	30.0	41.0
38	36.688772872805416	38.0	35.0	40.0	30.0	41.0
39	36.6177504607715	38.0	35.0	40.0	30.0	41.0
40	36.538351615339025	38.0	35.0	40.0	30.0	41.0
41	36.48551919001648	38.0	35.0	40.0	30.0	41.0
42	36.43084638514272	38.0	35.0	40.0	30.0	41.0
43	35.479029145657925	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	5.0
17	7.0
18	14.0
19	46.0
20	79.0
21	133.0
22	239.0
23	346.0
24	621.0
25	974.0
26	1501.0
27	2127.0
28	3027.0
29	4173.0
30	5593.0
31	7689.0
32	10641.0
33	14165.0
34	20464.0
35	29686.0
36	46059.0
37	72593.0
38	99978.0
39	49326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12875349469132	18.565370011015215	12.12864253063014	26.177233963663333
2	20.86070221305641	20.4942501671227	31.521371407538517	27.123676212282366
3	21.77656168383903	21.43960983953514	28.6428012741922	28.141027202433634
4	16.457052848663967	15.415343893864227	32.756590859267796	35.371012398204
5	16.896849432594745	34.01129668271586	32.99827599739099	16.093577887298405
6	36.94778464311522	32.71112265859065	14.669448887517625	15.671643810776507
7	30.5091626543686	28.30016590480366	20.49722725169085	20.69344418913689
8	28.671218899615415	30.410918863619756	19.63684981149642	21.28101242526841
9	27.03625818359951	13.716240537607344	19.29692088262438	39.95058039616876
10	18.37429531055052	25.423219635767236	32.34115223998549	23.86133281369675
11	37.0135511476661	20.801701809796775	20.15811025497376	22.026636787563366
12	23.564165644985373	24.267840179274618	27.24438345931814	24.92361071642187
13	32.54602978708432	18.75427955906671	22.640728140756558	26.058962513092403
14	24.3346892600321	20.075834463272248	23.23695698654087	32.35251929015478
15	26.881720430107524	26.58969549837749	20.749467507828378	25.779116563686603
16	26.445171574796543	24.79207770732011	22.776591454684713	25.986159263198633
17	25.528500171858976	24.770426183188132	23.67837743478155	26.022696210171343
18	25.166378430751664	23.71491438175426	25.01562969398277	26.103077493511307
19	26.434616456782205	23.992324534695214	24.969078917099022	24.603980091423562
20	27.00053316878175	23.66755167271556	23.94090216488177	25.39101299362092
21	26.743691963766175	23.627766997123054	24.03481565080422	25.593725388306552
22	26.83841738184357	23.835621628790033	23.926828674195985	25.39913231517041
23	26.41864845773487	23.61342286238562	23.55469310317763	26.413235576701876
24	25.926076283732396	24.040769819940515	24.3771803761411	25.655973520185988
25	26.295234770182603	23.987723585817168	23.666469096508962	26.050572547491264
26	26.041911937838474	24.13738974637946	23.87540630438254	25.94529201139953
27	25.992925364489878	23.450224499240843	24.110054697162838	26.44679543910644
28	25.76829080162062	23.874323728175938	24.010998974259042	26.3463864959444
29	25.820254459537363	24.076765478809925	24.553910941868367	25.549069119784352
30	26.080884681276036	24.03292114244267	23.671070045387008	26.215124130894292
31	25.66192768932228	24.46649291318551	24.393148375188435	25.478431022303777
32	25.483843903336773	23.831020679911987	24.131164933191513	26.553970483559723
33	24.883555396777712	24.204509471188587	24.696269713036113	26.21566541899759
34	26.528259298653005	22.91137219240627	24.298964245214336	26.26140426372639
35	25.48736227600822	23.14493800897997	24.616700361851095	26.750999353160715
36	25.05379050526538	24.015599923137092	24.810752146883942	26.119857424713587
37	25.53066532427217	22.73058196590426	25.564225186676737	26.174527523146836
38	25.348792521563563	22.975785476698903	25.53878464582166	26.13663735591587
39	24.861091940490788	22.429625780469785	26.59186065079069	26.117421628248742
40	24.133059441553065	22.118926409175916	27.20622264803553	26.54179150123549
41	23.583922660755803	22.43368544124453	27.38295321376279	26.599438684236876
42	23.54630313757649	22.314602058518656	27.893387895174143	26.245706908730703
43	23.623166048245007	22.16222945743987	27.691487432643463	26.52311706167166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	27.5
2	25.0
3	41.0
4	57.0
5	57.0
6	80.5
7	104.0
8	98.0
9	92.0
10	130.0
11	168.0
12	168.0
13	323.0
14	478.0
15	613.0
16	748.0
17	815.5
18	883.0
19	883.0
20	1186.5
21	1490.0
22	1707.0
23	1924.0
24	2411.5
25	2899.0
26	2899.0
27	3501.0
28	4103.0
29	5048.5
30	5994.0
31	7233.5
32	8473.0
33	8473.0
34	9634.0
35	10795.0
36	12199.5
37	13604.0
38	14599.5
39	15595.0
40	15595.0
41	16911.5
42	18228.0
43	19093.0
44	19958.0
45	20899.5
46	21841.0
47	21841.0
48	23077.0
49	24313.0
50	25111.5
51	25910.0
52	27148.0
53	28386.0
54	28386.0
55	27675.5
56	26965.0
57	27042.0
58	27119.0
59	26700.5
60	26282.0
61	26282.0
62	24540.5
63	22799.0
64	20905.0
65	19011.0
66	16684.5
67	14358.0
68	14358.0
69	12831.0
70	11304.0
71	9374.5
72	7445.0
73	5396.5
74	3348.0
75	3348.0
76	2642.5
77	1937.0
78	1561.5
79	1186.0
80	979.0
81	772.0
82	772.0
83	591.0
84	410.0
85	327.0
86	244.0
87	186.5
88	129.0
89	129.0
90	94.0
91	59.0
92	38.0
93	17.0
94	11.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	369489.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.67361040845189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.12577875769074	67.8723873276934
2	4.2763648225659345	6.301104718042624
3	1.205580447166722	2.664583925418249
4	0.6001607813447292	1.7686404634889463
5	0.37712371482937224	1.3892032821063642
6	0.26172682643677053	1.1569416146605884
7	0.19320973951789583	0.9964121353451951
8	0.13296235143540475	0.7836653182914939
9	0.10791088025340753	0.7155165735560387
>10	0.618972854467366	8.35890795265417
>50	0.06115685600326408	3.082567829563821
>100	0.03905196828840396	4.910068859179087
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	459	0.12422561970721725	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	456	0.12341368755226813	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	428	0.11583565410607623	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	415	0.11231728143462998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	2.7064405164971083E-4	0.0
18	0.0	0.0	0.0	5.412881032994217E-4	0.0
19	0.0	0.0	0.0	5.412881032994217E-4	0.0
20	0.0	0.0	0.0	8.119321549491324E-4	0.0
21	0.0	0.0	0.0	0.0010825762065988433	0.0
22	0.0	0.0	0.0	0.0016238643098982649	0.0
23	0.0	0.0	0.0	0.0018945083615479756	0.0
24	0.0	0.0	0.0	0.005142236981344505	0.0
25	0.0	0.0	0.0	0.007036745342892481	0.0
26	0.0	0.0	0.0	0.0100138299110393	0.0
27	0.0	0.0	0.0	0.03328921835291443	0.0
28	0.0	0.0	0.0	0.08092257144326354	0.0
29	0.0	0.0	0.0	0.14181748306444847	0.0
30	0.0	0.0	0.0	0.22652907123080795	0.0
31	0.0	0.0	0.0	0.41814505979880323	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	180	0.0	30.833332	1
CTTTTAG	55	1.898131E-5	23.545454	1
GCTTTAT	190	0.0	19.473684	1
GTATCAA	295	0.0	18.813559	2
ACTCTTT	50	0.00702607	18.5	8
GGACCTT	50	0.00702607	18.5	6
TCTTATA	265	0.0	16.754717	37
GAGCAAT	90	4.432834E-5	16.444445	11
TATTAGC	80	3.3747993E-4	16.1875	2
GGGTTAG	70	0.002588032	15.857142	1
TCAGGAC	85	5.3519255E-4	15.235294	3
ATCAACG	365	0.0	15.205479	4
CTTATAC	185	3.0195224E-10	15.000001	37
TCAACGC	375	0.0	14.800001	5
GTCTTGC	75	0.0040974002	14.8	1
TATACTT	75	0.0040974002	14.8	5
TTATTGA	200	6.184564E-11	14.8	4
CAACGCA	380	0.0	14.605264	6
ACAGGAC	90	8.2552957E-4	14.388888	3
AGAGTAA	105	1.6508374E-4	14.095238	33
>>END_MODULE