FastQCFastQC Report
Fri 10 Feb 2017
ERR1632239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815860
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17100.20959478341872378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13800.1691466673203736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10860.13311107297820704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.022.023811
GAACCGT450.003824871320.5555556
TACCCTT500.00703317518.55
TTAGACC701.2186468E-418.54
CTTATAC6550.018.07633637
CCATACA904.4434135E-516.4444452
TCCAACG904.4434135E-516.4444452
TAGAGTC1251.6558624E-716.2799995
CGAGCAA803.3810607E-416.187512
TCTTATA11850.016.08016837
GTGCTAT700.002591628615.8571431
TATACAC2055.456968E-1215.34146437
TATACTG855.361781E-415.2352945
GTTTAGA750.00410304914.82
GTATGAG1001.09274595E-414.81
GACGGAC1904.5474735E-1014.6052647
ATGTATA1152.2081806E-514.478261
GTAAACG908.270388E-414.38888827
GTATCAA9650.014.3782392
TTATACA3000.014.18333337
Produced by FastQC (version 0.11.5)