##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632239.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	815860
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.002729634986395	31.0	31.0	33.0	28.0	34.0
2	31.187305420047558	31.0	31.0	34.0	28.0	34.0
3	31.279346946780084	31.0	31.0	34.0	28.0	34.0
4	35.11555781629201	37.0	35.0	37.0	32.0	37.0
5	33.960080160811906	35.0	33.0	37.0	28.0	37.0
6	33.881610815581105	35.0	33.0	37.0	29.0	37.0
7	33.865290613585664	35.0	33.0	37.0	29.0	37.0
8	33.79696393989165	35.0	33.0	37.0	29.0	37.0
9	35.3043806535435	37.0	34.0	39.0	30.0	39.0
10	34.95338661044787	37.0	33.0	39.0	29.0	39.0
11	35.14952933101267	37.0	34.0	39.0	30.0	39.0
12	35.04526144191406	37.0	34.0	39.0	29.0	39.0
13	35.190931042090554	37.0	34.0	39.0	30.0	39.0
14	36.02265462211654	38.0	34.0	40.0	30.0	41.0
15	35.95571789277572	38.0	34.0	40.0	29.0	41.0
16	35.915033216483224	38.0	34.0	40.0	29.0	41.0
17	35.86336258671831	38.0	34.0	40.0	29.0	41.0
18	35.8491542666634	38.0	34.0	40.0	29.0	41.0
19	35.871197264236514	38.0	34.0	40.0	29.0	41.0
20	35.82706346677126	38.0	34.0	40.0	29.0	41.0
21	35.78895889000564	38.0	34.0	40.0	29.0	41.0
22	35.71726521707156	38.0	34.0	40.0	29.0	41.0
23	35.61803985977986	38.0	34.0	40.0	28.0	41.0
24	35.49963351555414	38.0	34.0	40.0	27.0	41.0
25	35.38563846738411	38.0	34.0	40.0	27.0	41.0
26	35.1782879415586	38.0	33.0	40.0	27.0	41.0
27	35.015628906920305	37.0	33.0	39.0	27.0	40.0
28	34.87675213884735	37.0	33.0	39.0	26.0	40.0
29	34.723593508690215	37.0	33.0	39.0	26.0	40.0
30	34.58444340940848	37.0	33.0	39.0	25.0	40.0
31	34.450724388988306	37.0	33.0	39.0	25.0	40.0
32	34.29363861446817	37.0	32.0	39.0	25.0	40.0
33	34.1427892040301	37.0	32.0	39.0	24.0	40.0
34	34.03232294756453	37.0	32.0	39.0	24.0	40.0
35	33.96786213320913	37.0	32.0	39.0	24.0	40.0
36	33.89797514279411	36.0	32.0	39.0	24.0	40.0
37	33.80996371926556	36.0	31.0	39.0	24.0	40.0
38	33.71839531292134	36.0	31.0	39.0	24.0	40.0
39	33.63119652881622	36.0	31.0	39.0	24.0	40.0
40	33.51147868506852	36.0	31.0	39.0	23.0	40.0
41	33.43477067143873	36.0	31.0	39.0	23.0	40.0
42	33.35153335131027	36.0	31.0	39.0	23.0	40.0
43	32.32020689824235	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	10.0
14	19.0
15	49.0
16	84.0
17	167.0
18	306.0
19	518.0
20	877.0
21	1496.0
22	2386.0
23	3687.0
24	5722.0
25	8112.0
26	11443.0
27	15336.0
28	20497.0
29	27230.0
30	35177.0
31	44446.0
32	55476.0
33	67929.0
34	82431.0
35	97666.0
36	108538.0
37	108912.0
38	85582.0
39	31761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8033731277425	19.23246635452161	12.787488049420242	26.176672468315644
2	20.457063711911356	20.83739857328463	32.38374230872944	26.32179540607457
3	21.184149241291397	21.796509205010665	28.779937734415217	28.23940381928272
4	16.382345010173314	15.832005491138185	32.45618120756012	35.32946829112838
5	16.81501728237688	34.390459147402744	32.08491652979678	16.709607040423602
6	36.04638050645944	33.3984997426029	14.921432598730174	15.633687152207488
7	30.57889834040154	29.17436815139852	20.102713700880052	20.144019807319882
8	28.27065918172235	31.813423871742703	19.180496653837668	20.735420292697277
9	27.24327703282426	13.840977618709092	19.012330546907556	39.90341480155909
10	18.238300688843676	25.661142843134854	31.187581202657316	24.912975265364157
11	38.227024244355654	20.476184639521485	20.153089990929814	21.143701125193047
12	23.20152967420881	24.241659108180325	27.30198808619126	25.254823131419606
13	32.046919814674084	18.291128379869097	23.060083837913368	26.601867967543452
14	23.98904223763881	19.672370259603365	23.525359743093176	32.81322775966465
15	26.765498982668596	26.377074498075647	20.69129507513544	26.166131444120317
16	26.67602284705709	24.554212732576666	22.592601671855466	26.177162748510774
17	25.438800774642704	24.939327825852477	23.7313999950972	25.890471404407617
18	25.723040717770207	23.39506778123698	24.787340965361704	26.09455053563111
19	26.102149878655652	24.18711543647194	24.37513789130488	25.33559679356752
20	26.517294633883264	23.369328070992573	24.16015002573971	25.953227269384456
21	27.166057902091044	23.729561444365455	23.630524844948887	25.473855808594614
22	27.164219351359304	23.673792072169245	23.517515259971074	25.64447331650038
23	26.262716642561223	23.33562130757728	23.867943029441328	26.533719020420172
24	26.046135366361877	23.991003358419334	23.850905792660505	26.11195548255828
25	26.321917976123355	23.187801828745126	23.94173019880862	26.5485499963229
26	25.98742431299488	24.031328904468904	23.98181060476062	25.999436177775596
27	26.22815188880445	23.549996322898537	23.98891966759003	26.232932120706987
28	25.86387370382173	23.693280709925723	24.358713504767977	26.08413208148457
29	25.423356948496068	23.84686098105067	24.581790993552815	26.14799107690045
30	25.41943470693501	24.292280538327653	24.35074645159709	25.937538303140244
31	26.046993356703357	23.985855416370455	23.825656362611234	26.141494864314957
32	25.42151839776432	23.724781212462922	24.046159880371633	26.80754050940112
33	25.11460299561199	23.462603878116344	24.80854558380114	26.61424754247052
34	26.017699115044245	23.074914813816097	24.711592675213883	26.19579339592577
35	25.72671781923369	23.047091412742382	25.296864658152135	25.92932610987179
36	24.965925526438358	23.329125094991788	25.087147304684628	26.617802073885226
37	25.13347878312456	22.367440492241318	25.791067094844706	26.708013629789424
38	24.47429706077023	22.25823057877577	26.616821513494966	26.65065084695904
39	24.546735959600912	21.89554580442723	27.009290809697745	26.548427426274117
40	24.07545412203074	22.096683254479935	27.733189517809425	26.094673105679895
41	23.490672419287623	22.02669575662491	28.38133993577329	26.101291888314172
42	23.499374892751206	22.229181477214226	28.24246807050229	26.028975559532274
43	22.682322947564533	21.74270095359498	28.475228593140976	27.099747505699508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	60.0
2	59.0
3	122.0
4	185.0
5	185.0
6	259.5
7	334.0
8	375.5
9	417.0
10	549.5
11	682.0
12	682.0
13	1178.0
14	1674.0
15	2639.0
16	3604.0
17	3396.5
18	3189.0
19	3189.0
20	3711.5
21	4234.0
22	4130.5
23	4027.0
24	4820.5
25	5614.0
26	5614.0
27	6696.5
28	7779.0
29	9427.0
30	11075.0
31	13022.5
32	14970.0
33	14970.0
34	18018.5
35	21067.0
36	24621.0
37	28175.0
38	31296.5
39	34418.0
40	34418.0
41	37765.5
42	41113.0
43	43407.0
44	45701.0
45	48669.0
46	51637.0
47	51637.0
48	54342.5
49	57048.0
50	58516.0
51	59984.0
52	61596.0
53	63208.0
54	63208.0
55	62642.0
56	62076.0
57	61032.5
58	59989.0
59	58540.0
60	57091.0
61	57091.0
62	52525.0
63	47959.0
64	43579.5
65	39200.0
66	34322.5
67	29445.0
68	29445.0
69	25266.0
70	21087.0
71	18134.0
72	15181.0
73	12193.5
74	9206.0
75	9206.0
76	7362.5
77	5519.0
78	4560.0
79	3601.0
80	2983.0
81	2365.0
82	2365.0
83	1812.0
84	1259.0
85	1036.5
86	814.0
87	638.0
88	462.0
89	462.0
90	370.5
91	279.0
92	169.0
93	59.0
94	33.5
95	8.0
96	8.0
97	6.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	815860.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.98985696978154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8039269589608	65.54775784059257
2	6.00306984656952	8.763264189614354
3	1.6566366165102364	3.6275300906995502
4	0.7593826799742126	2.217089327865887
5	0.44166934850091644	1.6118691287509244
6	0.2760925943635936	1.2091175383808768
7	0.19122261367590482	0.9770117855114459
8	0.13947994291548788	0.8144496866843802
9	0.09205923357539071	0.6047451262273931
>10	0.552220011025241	7.266425063222737
>50	0.0513221166475113	2.6507426140332724
>100	0.03207398505530182	4.0474732286089266
>500	3.3762089032713044E-4	0.14798061747240024
>1k	5.064313354906957E-4	0.51454376233534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1710	0.20959478341872378	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1380	0.1691466673203736	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1086	0.13311107297820704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2257004878287942E-4	0.0	0.0	0.0
3	0.0	1.2257004878287942E-4	0.0	0.0	0.0
4	0.0	1.2257004878287942E-4	0.0	0.0	0.0
5	0.0	3.677101463486382E-4	0.0	0.0	0.0
6	0.0	3.677101463486382E-4	0.0	0.0	0.0
7	0.0	3.677101463486382E-4	0.0	0.0	0.0
8	0.0	3.677101463486382E-4	0.0	0.0	0.0
9	0.0	3.677101463486382E-4	0.0	0.0	0.0
10	0.0	4.902801951315177E-4	0.0	1.2257004878287942E-4	0.0
11	0.0	4.902801951315177E-4	0.0	2.4514009756575883E-4	0.0
12	0.0	4.902801951315177E-4	0.0	2.4514009756575883E-4	0.0
13	0.0	4.902801951315177E-4	0.0	2.4514009756575883E-4	0.0
14	0.0	4.902801951315177E-4	0.0	2.4514009756575883E-4	0.0
15	0.0	4.902801951315177E-4	0.0	2.4514009756575883E-4	0.0
16	0.0	4.902801951315177E-4	0.0	2.4514009756575883E-4	0.0
17	0.0	4.902801951315177E-4	0.0	4.902801951315177E-4	0.0
18	0.0	4.902801951315177E-4	0.0	6.128502439143971E-4	0.0
19	0.0	4.902801951315177E-4	0.0	9.805603902630353E-4	0.0
20	0.0	4.902801951315177E-4	0.0	0.0011031304390459148	0.0
21	0.0	4.902801951315177E-4	0.0	0.0012257004878287942	0.0
22	0.0	4.902801951315177E-4	0.0	0.0017159806829603118	0.0
23	0.0	6.128502439143971E-4	0.0	0.0029416811707891058	0.0
24	0.0	6.128502439143971E-4	0.0	0.0045350918049665385	0.0
25	0.0	6.128502439143971E-4	0.0	0.005393082146446695	0.0
26	0.0	6.128502439143971E-4	0.0	0.008702473463584439	0.0
27	0.0	6.128502439143971E-4	0.0	0.028681391415193783	0.0
28	0.0	6.128502439143971E-4	0.0	0.09008898585541637	0.0
29	0.0	6.128502439143971E-4	0.0	0.18213909249135882	0.0
30	0.0	7.354202926972764E-4	0.0	0.31414703503051994	0.0
31	0.0	7.354202926972764E-4	0.0	0.7285563699654353	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	630	0.0	22.02381	1
GAACCGT	45	0.0038248713	20.555555	6
TACCCTT	50	0.007033175	18.5	5
TTAGACC	70	1.2186468E-4	18.5	4
CTTATAC	655	0.0	18.076336	37
CCATACA	90	4.4434135E-5	16.444445	2
TCCAACG	90	4.4434135E-5	16.444445	2
TAGAGTC	125	1.6558624E-7	16.279999	5
CGAGCAA	80	3.3810607E-4	16.1875	12
TCTTATA	1185	0.0	16.080168	37
GTGCTAT	70	0.0025916286	15.857143	1
TATACAC	205	5.456968E-12	15.341464	37
TATACTG	85	5.361781E-4	15.235294	5
GTTTAGA	75	0.004103049	14.8	2
GTATGAG	100	1.09274595E-4	14.8	1
GACGGAC	190	4.5474735E-10	14.605264	7
ATGTATA	115	2.2081806E-5	14.47826	1
GTAAACG	90	8.270388E-4	14.388888	27
GTATCAA	965	0.0	14.378239	2
TTATACA	300	0.0	14.183333	37
>>END_MODULE