FastQCFastQC Report
Fri 10 Feb 2017
ERR1632238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617757
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAT358.864735E-426.428571
TTATACT551.9008126E-523.5454544
TCTATAC400.001930181423.1250023
GGTATCA3000.022.8166681
GTATTAC450.003823835620.5555551
TAGCACT701.2180396E-418.54
CGGTGAT609.2302234E-418.514
GTTTTCG500.007031288518.528
TCGGTGA609.2302234E-418.513
AGTCGGT609.2302234E-418.511
TAATACT650.00157881617.0769234
TATATCT650.00157881617.0769235
GTACTAT650.00157881617.0769231
CTAGGAC1201.0388794E-716.9583343
ATACTGA904.440604E-516.4444456
AGCCGTT803.3793977E-416.187514
CATACCC700.002590674715.8571423
CCGACCA1059.329033E-615.8571429
TGTAGGA700.002590674715.8571422
GTATCAA4450.015.3820232