FastQCFastQC Report
Fri 10 Feb 2017
ERR1632237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189037
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.6596592201526685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12070.6384993413987737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8500.4496474235202632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.27825240561371584No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.13172024524299475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4750.029.9894731
GATTAAT250.005484874429.61
GTATCAA6300.022.317462
CGTCTGT450.003814169220.55555337
TACCCTC609.197946E-418.55
ATCAACG8250.017.2666664
ACAGAAC752.0557755E-417.2666663
CTTATAC2400.016.95833437
TATCAAC8350.016.8383243
TCAACGC8500.016.5411785
CAACGCA8700.016.3735646
ACTTTTC700.002581762815.8571428
AACGCAG9350.014.8395727
ACACTGA750.004087558514.7999996
TTACCCT908.229011E-414.3888874
GTCTCAC800.006272980513.8751
GGTTAGT850.00936676613.0588238
ATACACA1153.552594E-412.86956537
TCTTATA3750.012.33333337
CTCTTAT5550.012.33333337
Produced by FastQC (version 0.11.5)