##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189037
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.027465522622556	33.0	31.0	34.0	30.0	34.0
2	32.21123377963044	33.0	31.0	34.0	30.0	34.0
3	32.28061702206446	34.0	31.0	34.0	30.0	34.0
4	35.923983135576634	37.0	35.0	37.0	35.0	37.0
5	35.575247173833695	37.0	35.0	37.0	33.0	37.0
6	35.59988256267292	37.0	35.0	37.0	33.0	37.0
7	35.57970661828108	37.0	35.0	37.0	33.0	37.0
8	35.5617947809159	37.0	35.0	37.0	33.0	37.0
9	37.231610742870444	39.0	37.0	39.0	34.0	39.0
10	37.09056428106667	39.0	37.0	39.0	33.0	39.0
11	37.22402492633717	39.0	37.0	39.0	34.0	39.0
12	37.170194194787264	39.0	37.0	39.0	34.0	39.0
13	37.23268460671721	39.0	37.0	39.0	34.0	39.0
14	38.36266974190238	40.0	38.0	41.0	34.0	41.0
15	38.34313917381253	40.0	38.0	41.0	34.0	41.0
16	38.324074123055276	40.0	38.0	41.0	34.0	41.0
17	38.33757412570026	40.0	38.0	41.0	34.0	41.0
18	38.36593894316986	40.0	38.0	41.0	34.0	41.0
19	38.41880690023646	40.0	38.0	41.0	34.0	41.0
20	38.392039653612784	40.0	38.0	41.0	34.0	41.0
21	38.385183852896525	40.0	38.0	41.0	34.0	41.0
22	38.3277347820797	40.0	38.0	41.0	34.0	41.0
23	38.2636203494554	40.0	38.0	41.0	34.0	41.0
24	38.22022672810085	40.0	38.0	41.0	34.0	41.0
25	38.12949845797384	40.0	38.0	41.0	34.0	41.0
26	38.015034093854645	40.0	37.0	41.0	34.0	41.0
27	37.89845374186006	40.0	37.0	41.0	33.0	41.0
28	37.84011595613557	40.0	37.0	41.0	33.0	41.0
29	37.72387416220105	39.0	37.0	41.0	33.0	41.0
30	37.621904706486035	39.0	37.0	40.0	33.0	41.0
31	37.49478673487201	39.0	37.0	40.0	33.0	41.0
32	37.372419156038234	39.0	36.0	40.0	33.0	41.0
33	37.26336114094066	39.0	36.0	40.0	32.0	41.0
34	37.16841676497194	39.0	36.0	40.0	32.0	41.0
35	37.13042420266932	39.0	36.0	40.0	32.0	41.0
36	37.060078185752	39.0	36.0	40.0	31.0	41.0
37	36.96541946814645	39.0	36.0	40.0	31.0	41.0
38	36.892608325354296	39.0	36.0	40.0	31.0	41.0
39	36.8256002793104	39.0	35.0	40.0	31.0	41.0
40	36.69600131191248	39.0	35.0	40.0	31.0	41.0
41	36.62342292778662	39.0	35.0	40.0	31.0	41.0
42	36.52138470246565	39.0	35.0	40.0	30.0	41.0
43	35.69180107597983	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	2.0
18	9.0
19	20.0
20	25.0
21	45.0
22	96.0
23	140.0
24	279.0
25	434.0
26	669.0
27	973.0
28	1404.0
29	1958.0
30	2617.0
31	3555.0
32	4695.0
33	6266.0
34	8751.0
35	12781.0
36	20339.0
37	35643.0
38	54749.0
39	33584.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.05228077043119	21.391050429281037	14.081370313748101	23.47529848653967
2	18.87831482725604	22.06816654940567	35.48511667028148	23.56840195305681
3	20.009839343620563	24.141834667287355	30.9680115532938	24.880314435798283
4	14.75319646418426	17.050630299888383	36.17175473584536	32.024418500082
5	14.08401529859234	36.83194295296688	34.40120188111322	14.68283986732756
6	31.740876124779803	36.13049297227527	16.818400630564387	15.31023027238054
7	26.75243470854912	31.676338494580424	22.686564005988245	18.88466279088221
8	26.087485518707975	33.63785925506647	21.27678708401001	18.997868142215545
9	25.78384125858959	14.383427582959948	22.7965953755085	37.03613578294196
10	17.45266799621238	27.44753672561456	33.87484989711009	21.224945381062966
11	32.99195395610383	22.85478504208171	22.49929907901628	21.65396192279818
12	20.427746949009983	28.32514269693235	29.702121806842047	21.544988547215624
13	29.848124970243923	21.140834863016238	25.80500113734348	23.20603902939636
14	22.283997312695398	22.818813248200087	27.16663933515661	27.730550103947905
15	25.058057417330996	28.32091072118157	24.385173272957143	22.235858588530288
16	23.06585483265181	27.04761501716595	26.42286959695721	23.46366055322503
17	21.03080349349598	27.781333812957254	27.782391806894946	23.40547088665182
18	21.21648142956141	26.069499621767168	29.312251040801534	23.40176790786989
19	22.071869528187605	26.894734893169066	29.898908679253267	21.13448689939007
20	23.193343102144024	26.2128578003248	29.54871268587631	21.04508641165486
21	23.93446785549919	26.026121870321685	28.1928934547205	21.846516819458625
22	23.560995995492945	26.088543512645675	28.75045625988563	21.60000423197575
23	22.76802953919074	26.2128578003248	28.831392796119275	22.187719864365178
24	22.38979670646487	26.848183159910494	28.309801784835777	22.45221834878886
25	22.433703454879204	26.6260044329946	28.51134962996662	22.428942482159577
26	22.04224569793215	26.6260044329946	28.62402598433111	22.70772388474214
27	22.069753540312213	26.421811603019513	29.022889698842025	22.485545157826245
28	21.960780164729655	26.324476160751598	29.194284716748577	22.520458957770174
29	21.833820892206287	26.536603945259397	29.680432931119306	21.949142231415014
30	21.983527034390093	27.135428513994615	28.776377111359153	22.10466734025614
31	22.39878965493528	27.3094685167454	28.371694430190914	21.92004739812841
32	21.297946962763902	26.787877505461893	29.124457116860718	22.78971841491348
33	21.11385601760502	26.36996990007247	29.712701746218993	22.803472336103514
34	21.91369943450224	25.484957971190823	29.78993530367071	22.811407290636225
35	21.69046271364865	25.912916518988343	29.642874146331145	22.753746621031862
36	21.868205695181366	26.088543512645675	29.05568751091056	22.9875632812624
37	21.648142956140862	25.130001005094243	29.83807402783582	23.383782010929078
38	21.47357395642123	25.033194559795174	30.430021635976022	23.063209847807574
39	20.817088718081646	24.634330845284254	31.348360373895055	23.200220062739042
40	20.300258679517764	24.241286097430663	32.24289424821596	23.215560974835615
41	19.982860498209345	24.212191264144057	32.304786893571105	23.500161344075497
42	19.532684077720237	24.63327285134656	32.3248887783873	23.509154292545904
43	19.072456714823023	24.237583118648732	32.24024926337172	24.449710903156525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	96.5
2	86.0
3	151.5
4	217.0
5	217.0
6	300.0
7	383.0
8	373.5
9	364.0
10	520.5
11	677.0
12	677.0
13	1179.5
14	1682.0
15	2186.0
16	2690.0
17	2434.0
18	2178.0
19	2178.0
20	2481.0
21	2784.0
22	2422.5
23	2061.0
24	2401.5
25	2742.0
26	2742.0
27	3175.0
28	3608.0
29	4234.5
30	4861.0
31	5791.0
32	6721.0
33	6721.0
34	7826.0
35	8931.0
36	9679.0
37	10427.0
38	10846.5
39	11266.0
40	11266.0
41	11975.0
42	12684.0
43	13083.0
44	13482.0
45	13535.0
46	13588.0
47	13588.0
48	13812.5
49	14037.0
50	13638.0
51	13239.0
52	13021.5
53	12804.0
54	12804.0
55	12444.5
56	12085.0
57	11186.5
58	10288.0
59	9367.5
60	8447.0
61	8447.0
62	7296.0
63	6145.0
64	5085.0
65	4025.0
66	3276.0
67	2527.0
68	2527.0
69	2010.0
70	1493.0
71	1235.0
72	977.0
73	751.0
74	525.0
75	525.0
76	427.5
77	330.0
78	266.5
79	203.0
80	169.0
81	135.0
82	135.0
83	122.5
84	110.0
85	87.5
86	65.0
87	48.5
88	32.0
89	32.0
90	27.0
91	22.0
92	14.0
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	189037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.44899923821374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.78784092528078	65.94827059721804
2	5.1930293195276285	7.628456130680128
3	1.7082289979326197	3.764031311035428
4	0.9067693993628131	2.664052196921392
5	0.6340166522824753	2.3283944305255178
6	0.3788661073791499	1.669640185936571
7	0.2899366258967579	1.4906888510234864
8	0.1955031376332301	1.1487607845673211
9	0.14783905170617143	0.9772767356530343
>10	0.7381278239637792	8.578145139694616
>50	0.011905175420741515	0.5669177212317342
>100	0.003968391806913839	0.4444984703744959
>500	0.0019841959034569193	1.0026719594021416
>1k	0.0019841959034569193	1.7881954857360869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1247	0.6596592201526685	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.6384993413987737	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	850	0.4496474235202632	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	526	0.27825240561371584	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.13172024524299475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	5.289969688473685E-4	0.0
14	0.0	0.0	0.0	5.289969688473685E-4	0.0
15	0.0	0.0	0.0	0.001057993937694737	0.0
16	0.0	0.0	0.0	0.0026449848442368427	0.0
17	0.0	0.0	0.0	0.004231975750778948	0.0
18	0.0	0.0	0.0	0.004231975750778948	0.0
19	0.0	0.0	0.0	0.004231975750778948	0.0
20	0.0	0.0	0.0	0.004231975750778948	0.0
21	0.0	0.0	0.0	0.0047609727196263165	0.0
22	0.0	0.0	0.0	0.0058189666573210536	0.0
23	0.0	0.0	0.0	0.008463951501557897	0.0
24	0.0	0.0	0.0	0.012695927252336843	0.0
25	0.0	0.0	0.0	0.014811915127726318	0.0
26	0.0	0.0	0.0	0.0185148939096579	0.0
27	0.0	0.0	0.0	0.05289969688473685	0.0
28	0.0	0.0	0.0	0.13172024524299475	0.0
29	0.0	0.0	0.0	0.2502155662648053	0.0
30	0.0	0.0	0.0	0.40574067510593165	0.0
31	0.0	0.0	0.0	0.8998238440093739	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	475	0.0	29.989473	1
GATTAAT	25	0.0054848744	29.6	1
GTATCAA	630	0.0	22.31746	2
CGTCTGT	45	0.0038141692	20.555553	37
TACCCTC	60	9.197946E-4	18.5	5
ATCAACG	825	0.0	17.266666	4
ACAGAAC	75	2.0557755E-4	17.266666	3
CTTATAC	240	0.0	16.958334	37
TATCAAC	835	0.0	16.838324	3
TCAACGC	850	0.0	16.541178	5
CAACGCA	870	0.0	16.373564	6
ACTTTTC	70	0.0025817628	15.857142	8
AACGCAG	935	0.0	14.839572	7
ACACTGA	75	0.0040875585	14.799999	6
TTACCCT	90	8.229011E-4	14.388887	4
GTCTCAC	80	0.0062729805	13.875	1
GGTTAGT	85	0.009366766	13.058823	8
ATACACA	115	3.552594E-4	12.869565	37
TCTTATA	375	0.0	12.333333	37
CTCTTAT	555	0.0	12.333333	37
>>END_MODULE