FastQCFastQC Report
Fri 10 Feb 2017
ERR1632236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106812
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2130.19941579597798No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG1540.1441785567164738No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC1530.14324233232221098No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC1330.12451784443695466No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG1240.1160918248885893No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA1230.11515560049432648No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT1070.1001760101861214No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC1070.1001760101861214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAAGG250.005474324329.5999983
ATGACGG250.005474324329.59999820
TTATAAA508.990697E-625.8999983
CTTATTT451.3122661E-424.66666624
ACTTTTG451.3122661E-424.66666630
GGTTAAT451.3122661E-424.6666661
GGTATCA551.881795E-523.5454561
TTTATAA551.881795E-523.5454562
TCAGACC400.001919801623.12500219
GATGTGC502.6799302E-422.19999936
TTTGATG502.6799302E-422.19999933
TGTATTC450.00380346920.5555553
TTCTTAT450.00380346920.55555522
ACCAAAA450.00380346920.55555523
TTGATGT555.101233E-420.1818234
TGGGAAC555.101233E-420.1818225
ACGAGAC555.101233E-420.1818219
TTGGGAA555.101233E-420.1818224
TTAATTC656.827514E-519.9230773
AACTTTT609.162251E-418.529