##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632236.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	106812
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.955463805564918	31.0	31.0	33.0	28.0	34.0
2	31.159841590832492	31.0	31.0	34.0	28.0	34.0
3	31.247191326817212	31.0	31.0	34.0	28.0	34.0
4	35.08614200651612	37.0	35.0	37.0	32.0	37.0
5	33.89195034265813	35.0	33.0	37.0	28.0	37.0
6	33.85762835636445	35.0	33.0	37.0	29.0	37.0
7	33.848238025689994	35.0	33.0	37.0	29.0	37.0
8	33.75510242294873	35.0	33.0	37.0	29.0	37.0
9	35.28117627232895	37.0	34.0	39.0	30.0	39.0
10	34.92661873197768	37.0	33.0	39.0	29.0	39.0
11	35.14042429689548	37.0	34.0	39.0	29.0	39.0
12	35.06087331011497	37.0	34.0	39.0	29.0	39.0
13	35.16837995730817	37.0	34.0	39.0	30.0	39.0
14	36.03324532824027	38.0	34.0	40.0	30.0	41.0
15	35.978363854248585	38.0	34.0	40.0	29.0	41.0
16	35.92795753286148	38.0	34.0	40.0	29.0	41.0
17	35.860877055012544	38.0	34.0	40.0	29.0	41.0
18	35.86450024341834	38.0	34.0	40.0	29.0	41.0
19	35.89724001048571	38.0	34.0	40.0	29.0	41.0
20	35.83425083323971	38.0	34.0	40.0	29.0	41.0
21	35.78728981762349	38.0	34.0	40.0	29.0	41.0
22	35.7121578099839	38.0	34.0	40.0	28.0	41.0
23	35.61732764108902	38.0	34.0	40.0	27.0	41.0
24	35.523705201662736	38.0	34.0	40.0	27.0	41.0
25	35.431009624386775	38.0	34.0	40.0	27.0	41.0
26	35.2247874770625	38.0	34.0	40.0	27.0	41.0
27	35.08823914915927	38.0	33.0	40.0	27.0	41.0
28	34.96004194285286	37.0	33.0	40.0	26.0	41.0
29	34.862740141557126	37.0	33.0	39.0	26.0	40.0
30	34.73770737370333	37.0	33.0	39.0	26.0	40.0
31	34.59824738793394	37.0	33.0	39.0	25.0	40.0
32	34.47159495187807	37.0	33.0	39.0	25.0	40.0
33	34.33961539901884	37.0	32.0	39.0	25.0	40.0
34	34.244775867880016	37.0	32.0	39.0	25.0	40.0
35	34.19433209751713	37.0	32.0	39.0	24.0	40.0
36	34.12514511478111	37.0	32.0	39.0	24.0	40.0
37	34.06341047822342	37.0	32.0	39.0	24.0	40.0
38	33.966604875856646	37.0	32.0	39.0	24.0	40.0
39	33.88971276635584	37.0	31.0	39.0	24.0	40.0
40	33.80302774969105	36.0	31.0	39.0	24.0	40.0
41	33.71405834550425	36.0	31.0	39.0	24.0	40.0
42	33.673032992547654	36.0	31.0	39.0	24.0	40.0
43	32.593528816986854	35.0	30.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	3.0
16	14.0
17	23.0
18	39.0
19	73.0
20	103.0
21	191.0
22	333.0
23	506.0
24	721.0
25	1094.0
26	1449.0
27	2000.0
28	2734.0
29	3542.0
30	4495.0
31	5743.0
32	7072.0
33	8751.0
34	10389.0
35	12223.0
36	13616.0
37	14312.0
38	12353.0
39	5030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58708759315432	19.43976332247313	12.949855821443284	26.023293262929258
2	19.42946485413624	21.60899524398008	33.645096056622855	25.31644384526083
3	20.448076995094183	22.396359959555106	30.848593790959818	26.306969254390893
4	16.3586488409542	16.084335093435197	33.66288432011385	33.89413174549676
5	15.53758004718571	36.02965958881025	33.41010373366288	15.02265663034116
6	35.05785866756545	35.070965809085116	15.18930457251994	14.681870950829495
7	29.94981837246751	30.30183874471033	20.428416282814666	19.319926600007488
8	27.63828034303262	33.66850166647942	19.691607684529828	19.001610305958135
9	27.385499756581655	14.279294461296482	18.526944538066882	39.808261244054975
10	17.015878365726696	27.121484477399544	31.968318166498143	23.894318990375613
11	37.378758940942966	21.10811519304947	20.961127963150208	20.55199790285736
12	22.739954312249562	24.656405647305547	27.883571134329475	24.720068906115415
13	31.503950866943793	18.593416470059545	24.063775605737185	25.838857057259485
14	23.309178743961354	19.969666329625884	23.64996442347302	33.07119050293974
15	26.121596824326854	27.298430887915217	20.91806164101412	25.661910646743813
16	26.34629067894993	25.48496423622814	22.496535969741227	25.6722091150807
17	25.271505074336215	25.64505860764708	23.795079204583754	25.288357113432948
18	24.781859716136765	24.201400591693815	25.24154589371981	25.775193798449614
19	26.118788151144066	24.748155637943302	25.07957907351234	24.053477137400293
20	26.274201400591696	23.964535819945322	24.603977081226827	25.157285698236155
21	26.646818709508295	24.90263266299667	23.244579260757217	25.20596936673782
22	26.680522787701754	24.396135265700483	23.51233943751638	25.411002509081378
23	25.68250758341759	24.765007677040032	23.92708684417481	25.625397895367563
24	26.898663071564993	24.45792607572183	23.372842002771225	25.270568849941956
25	26.31258660075647	23.68647717484927	24.21918885518481	25.78174736920945
26	26.161854473280155	24.08250009362244	23.728607272591095	26.02703816050631
27	25.996142755495637	24.008538366475676	23.9373853125117	26.057933565516983
28	25.466239748342883	24.495375051492342	24.46728831966446	25.571096880500317
29	25.44845148485189	24.858630116466315	24.139609781672473	25.553308617009325
30	24.95693367786391	25.539265251095383	24.07126540089129	25.43253567014942
31	25.95494888214807	24.47196944163577	24.296895479908624	25.27618619630753
32	24.75377298430888	24.911058682545033	24.39519904130622	25.93996929183987
33	25.54581882185522	24.267872523686478	24.47196944163577	25.71433921282253
34	25.64037748567577	23.94955622963712	24.513163314983334	25.896902969703778
35	25.329550986780514	24.158334269557727	24.72100513050968	25.79110961315208
36	25.044002546530354	24.67138523761375	24.649852076545706	25.634760139310192
37	26.056061116728458	23.277347114556417	24.72100513050968	25.94558663820544
38	24.76032655506872	23.993558776167472	25.79110961315208	25.45500505561173
39	24.995318878028687	23.199640489832603	26.12721417069243	25.67782646144628
40	24.78934951129087	22.739018087855296	26.812530427292813	25.65910197356102
41	23.59472718421151	23.141594577388307	27.616747181964573	25.646931056435605
42	24.463543422087405	22.0087630603303	27.38737220537018	26.140321312212112
43	23.28858180728757	21.94603602591469	28.344193536306783	26.421188630490956
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	3.0
4	6.0
5	6.0
6	10.0
7	14.0
8	14.0
9	14.0
10	20.5
11	27.0
12	27.0
13	41.5
14	56.0
15	120.0
16	184.0
17	190.0
18	196.0
19	196.0
20	270.0
21	344.0
22	424.5
23	505.0
24	626.0
25	747.0
26	747.0
27	886.0
28	1025.0
29	1447.5
30	1870.0
31	2148.5
32	2427.0
33	2427.0
34	2749.5
35	3072.0
36	3438.0
37	3804.0
38	4497.0
39	5190.0
40	5190.0
41	5871.0
42	6552.0
43	6496.0
44	6440.0
45	6900.5
46	7361.0
47	7361.0
48	7706.5
49	8052.0
50	8455.5
51	8859.0
52	9415.5
53	9972.0
54	9972.0
55	8928.5
56	7885.0
57	7529.0
58	7173.0
59	6671.0
60	6169.0
61	6169.0
62	5688.0
63	5207.0
64	4709.0
65	4211.0
66	3646.5
67	3082.0
68	3082.0
69	2642.0
70	2202.0
71	1919.5
72	1637.0
73	1313.0
74	989.0
75	989.0
76	809.0
77	629.0
78	514.5
79	400.0
80	320.0
81	240.0
82	240.0
83	185.0
84	130.0
85	99.0
86	68.0
87	55.5
88	43.0
89	43.0
90	34.0
91	25.0
92	14.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	106812.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.68063513462907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.76188826760354	70.20278620379733
2	3.645652926913751	5.518106579785043
3	1.1343955663318324	2.575553308617009
4	0.5925577712900193	1.7938059394075574
5	0.383492503340096	1.4511478111073661
6	0.24865159087535255	1.1290866194809572
7	0.1707160176159137	0.9043927648578811
8	0.11257360581918947	0.6815713590233307
9	0.0977287347221535	0.6656555443208628
>10	0.7830669503686476	11.079279481706175
>50	0.058142411796724226	2.859229300078643
>100	0.01113365332277698	1.1393850878178482
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	213	0.19941579597798	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	154	0.1441785567164738	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	153	0.14324233232221098	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	133	0.12451784443695466	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	124	0.1160918248885893	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	123	0.11515560049432648	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	107	0.1001760101861214	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	107	0.1001760101861214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.002808673182788451	0.0
24	0.0	0.0	0.0	0.004681121971314085	0.0
25	0.0	0.0	0.0	0.004681121971314085	0.0
26	0.0	0.0	0.0	0.004681121971314085	0.0
27	0.0	0.0	0.0	0.011234692731153803	0.0
28	0.0	0.0	0.0	0.05055611729019211	0.0
29	0.0	0.0	0.0	0.11234692731153803	0.0
30	0.0	0.0	0.0	0.19005355203535182	0.0
31	0.0	0.0	0.0	0.3726173089166011	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAAGG	25	0.0054743243	29.599998	3
ATGACGG	25	0.0054743243	29.599998	20
TTATAAA	50	8.990697E-6	25.899998	3
CTTATTT	45	1.3122661E-4	24.666666	24
ACTTTTG	45	1.3122661E-4	24.666666	30
GGTTAAT	45	1.3122661E-4	24.666666	1
GGTATCA	55	1.881795E-5	23.545456	1
TTTATAA	55	1.881795E-5	23.545456	2
TCAGACC	40	0.0019198016	23.125002	19
GATGTGC	50	2.6799302E-4	22.199999	36
TTTGATG	50	2.6799302E-4	22.199999	33
TGTATTC	45	0.003803469	20.555555	3
TTCTTAT	45	0.003803469	20.555555	22
ACCAAAA	45	0.003803469	20.555555	23
TTGATGT	55	5.101233E-4	20.18182	34
TGGGAAC	55	5.101233E-4	20.18182	25
ACGAGAC	55	5.101233E-4	20.18182	19
TTGGGAA	55	5.101233E-4	20.18182	24
TTAATTC	65	6.827514E-5	19.923077	3
AACTTTT	60	9.162251E-4	18.5	29
>>END_MODULE