FastQCFastQC Report
Fri 10 Feb 2017
ERR1632235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15106
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3942.6082351383556204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3792.508936846286244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2151.4232755196610618No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1521.006222692969681TruSeq Adapter, Index 1 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.9267840593141797No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1100.7281874751754269No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.5229710048987157No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT770.5097312326227988No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC770.5097312326227988No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG430.2846551039322124No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.2714153316562955No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.21183635641466966No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC310.20521647027671122RNA PCR Primer, Index 1 (95% over 22bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.19859658413875278No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG230.15225738117304383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC200.13239772275916853No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA200.13239772275916853No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA190.12577783662121012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG180.11915795048325169No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC180.11915795048325169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1300.022.769231
GTATCAA1706.002665E-1116.323532
TATCAAC2006.9485395E-1013.8749993
CAACGCA2151.3278623E-1013.7674426
CTGTCTC1355.6031604E-613.70370437
ATCAACG2201.9099389E-1013.4545454
TCAACGC2252.746674E-1013.1555565
AACGCAG2355.475158E-1012.5957447
CGCAGAG2901.2332748E-910.8448289
AGTACAT1758.0441496E-510.57142914
GAGTACA1758.0441496E-510.57142913
ACATGGG1801.07084445E-410.27777917
GTACATG1901.8507887E-49.73684215
TACATGG1901.8507887E-49.73684216
ACGCAGA3053.288551E-89.7049188
CAGAGTA3053.288551E-89.70491811
AGAGTAC3053.288551E-89.70491812
GCAGAGT3206.919436E-89.2510
Produced by FastQC (version 0.11.5)