##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632235.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15106
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.3534357209056	31.0	30.0	31.0	27.0	34.0
2	30.44776909837151	31.0	30.0	33.0	27.0	34.0
3	30.394015622931285	31.0	30.0	34.0	26.0	34.0
4	34.22024361180988	35.0	35.0	37.0	30.0	37.0
5	32.88699854362505	35.0	32.0	37.0	26.0	37.0
6	32.982457301734414	35.0	32.0	37.0	26.0	37.0
7	32.986495432278566	35.0	32.0	37.0	26.0	37.0
8	32.92546008208659	35.0	32.0	37.0	26.0	37.0
9	34.13478088176883	37.0	32.0	39.0	27.0	39.0
10	33.80113862041573	35.0	32.0	39.0	26.0	39.0
11	34.15106580166821	37.0	32.0	39.0	27.0	39.0
12	33.99245332980273	37.0	32.0	39.0	27.0	39.0
13	34.22434794121541	37.0	32.0	39.0	27.0	39.0
14	34.638024625976435	37.0	32.0	39.0	26.0	41.0
15	34.60684496226665	37.0	32.0	39.0	26.0	41.0
16	34.52899510128426	37.0	32.0	39.0	26.0	41.0
17	34.62107771746326	37.0	32.0	39.0	27.0	40.0
18	34.66999867602277	37.0	32.0	39.0	26.0	41.0
19	34.68019330067523	37.0	32.0	39.0	26.0	41.0
20	34.587845889050705	37.0	32.0	39.0	25.0	41.0
21	34.630808950086056	37.0	32.0	40.0	25.0	41.0
22	34.55137031643056	37.0	32.0	39.0	25.0	41.0
23	34.45981729114259	37.0	32.0	39.0	25.0	41.0
24	34.26850258175559	37.0	32.0	39.0	25.0	41.0
25	34.14351913147094	37.0	32.0	39.0	25.0	41.0
26	33.73050443532371	36.0	31.0	39.0	24.0	40.0
27	33.478948762081295	36.0	31.0	39.0	23.0	40.0
28	33.33370846021448	36.0	31.0	39.0	23.0	40.0
29	33.087845889050705	35.0	30.0	39.0	22.0	40.0
30	32.79048060373361	35.0	30.0	38.0	21.0	40.0
31	32.41870779822587	35.0	30.0	38.0	20.0	40.0
32	32.156957500330996	35.0	30.0	38.0	20.0	40.0
33	31.73613133854098	35.0	30.0	38.0	18.0	40.0
34	31.56077055474646	35.0	29.0	38.0	17.0	40.0
35	31.31053885873163	35.0	28.0	38.0	16.0	40.0
36	31.002383159009664	34.0	27.0	38.0	15.0	40.0
37	30.71958162319608	34.0	27.0	38.0	15.0	40.0
38	30.46153846153846	34.0	26.0	38.0	14.0	40.0
39	30.150734807361314	34.0	26.0	38.0	12.0	40.0
40	29.779160598437706	33.0	25.0	37.0	10.0	40.0
41	29.511584800741428	33.0	24.0	37.0	10.0	40.0
42	29.211108168939493	33.0	24.0	37.0	9.0	40.0
43	28.226929696809215	32.0	22.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	5.0
16	6.0
17	9.0
18	23.0
19	39.0
20	78.0
21	107.0
22	149.0
23	204.0
24	277.0
25	381.0
26	451.0
27	585.0
28	735.0
29	808.0
30	971.0
31	1110.0
32	1211.0
33	1145.0
34	1331.0
35	1370.0
36	1426.0
37	1245.0
38	917.0
39	519.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77810141665563	20.998278829604132	17.38382099827883	19.839798755461405
2	20.296570898980537	25.23500595789752	30.49781543757447	23.970607705547465
3	22.646630477955778	24.685555408446973	32.801535813584	19.866278300013242
4	15.814907983582682	20.011915795048324	28.650867205084076	35.52230901628492
5	13.259631934330729	37.72011121408711	31.974050046339205	17.04620680524295
6	29.696809214881505	32.70885740765259	16.463656825102607	21.1306765523633
7	23.500595789752417	33.97987554614061	20.846021448431088	21.67350721567589
8	31.040646100887066	31.43783926916457	19.462465245597777	18.05904938435059
9	24.731894611412685	12.816099563087516	21.567589037468554	40.884416788031245
10	20.52826691380908	27.882960413080895	27.969018932874356	23.61975374023567
11	31.66291539785516	22.534092413610484	19.204289686217397	26.59870250231696
12	19.045412418906395	32.046868793856746	27.505626903217266	21.402091884019594
13	33.86733748179531	17.675095988349003	27.472527472527474	20.985039057328216
14	21.93168277505627	23.328478750165495	29.21355752681054	25.526280947967695
15	28.412551304117567	26.221368992453332	24.976830398517144	20.389249304911957
16	22.692969680921486	23.9176486164438	29.776247848537007	23.613133854097708
17	18.714418112008474	30.815569972196478	28.895802992188536	21.574208923606513
18	19.111611280285977	21.898583344366475	33.609161922414934	25.38064345293261
19	21.567589037468554	26.744339997352046	35.05891698662783	16.62915397855157
20	21.660267443399974	22.40169469085132	35.39653117966371	20.541506686084997
21	25.863895141003574	20.567986230636834	34.185092016417315	19.383026611942274
22	23.798490665960546	23.970607705547465	32.73533695220443	19.495564676287568
23	22.222957765126438	23.076923076923077	33.06633125910234	21.63378789884814
24	20.95193962663842	25.45346220045015	34.19171190255528	19.402886270356152
25	19.601482854494904	25.162187210379983	34.026214749106316	21.2101151860188
26	22.65325036409374	22.72606911161128	34.57566529855686	20.045015225738116
27	21.031378260293923	24.222163378789887	34.23805110552098	20.50840725539521
28	20.534886799947042	23.725671918443002	35.31047265987025	20.428968621739706
29	19.66106182973653	24.16258440354826	35.667946511320004	20.50840725539521
30	19.26386866145902	24.851052561895937	36.89924533298027	18.985833443664767
31	20.58122600291275	24.80471335893023	34.32410962531445	20.28995101284258
32	19.866278300013242	25.671918443002777	35.403151065801666	19.05865219118231
33	19.495564676287568	25.029789487620814	35.49582947173309	19.978816364358533
34	20.382629418774	26.347146829074543	32.90083410565338	20.369389646498078
35	19.204289686217397	24.771613928240434	36.15781808552893	19.866278300013242
36	20.111214087117702	25.307824705415065	32.35138355620283	22.2295776512644
37	19.038792532768436	24.943730967827353	33.80113862041573	22.216337878988483
38	19.641202171322654	24.08314576989276	34.33734939759036	21.93830266119423
39	20.521647027671126	23.944128160995632	34.29763008076261	21.236594730570634
40	18.27088574076526	23.275519661061832	35.840063550906926	22.613531047265987
41	18.913014696147226	23.685952601615252	34.741162452005824	22.659870250231695
42	18.509201641731764	23.53369522044221	35.53554878856084	22.421554349265193
43	17.357341453726995	24.334701443135177	34.4896067787634	23.81835032437442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	11.5
4	19.0
5	19.0
6	44.5
7	70.0
8	75.5
9	81.0
10	110.0
11	139.0
12	139.0
13	276.0
14	413.0
15	613.0
16	813.0
17	692.5
18	572.0
19	572.0
20	622.5
21	673.0
22	460.5
23	248.0
24	214.0
25	180.0
26	180.0
27	193.0
28	206.0
29	201.5
30	197.0
31	212.5
32	228.0
33	228.0
34	245.0
35	262.0
36	291.0
37	320.0
38	373.5
39	427.0
40	427.0
41	490.5
42	554.0
43	678.5
44	803.0
45	885.5
46	968.0
47	968.0
48	1173.0
49	1378.0
50	1354.0
51	1330.0
52	1178.0
53	1026.0
54	1026.0
55	1057.0
56	1088.0
57	1018.0
58	948.0
59	751.5
60	555.0
61	555.0
62	514.0
63	473.0
64	406.5
65	340.0
66	302.0
67	264.0
68	264.0
69	236.5
70	209.0
71	170.0
72	131.0
73	104.0
74	77.0
75	77.0
76	63.0
77	49.0
78	40.0
79	31.0
80	24.0
81	17.0
82	17.0
83	10.5
84	4.0
85	5.0
86	6.0
87	3.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	15106.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.2143519131471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.99862825788752	72.58043161657619
2	3.3521947873799722	5.17675095988349
3	1.0459533607681755	2.4228783264927842
4	0.4029492455418381	1.2445385939361844
5	0.3000685871056241	1.1584800741427248
6	0.1800411522633745	0.8341056533827619
7	0.16289437585733882	0.8804448563484708
8	0.1286008230452675	0.7943863365550111
9	0.07716049382716049	0.5362107771746326
>10	0.2743484224965706	3.6276976036012183
>50	0.0257201646090535	1.5424334701443136
>100	0.051440329218107	9.201641731762214
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	394	2.6082351383556204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	379	2.508936846286244	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	215	1.4232755196610618	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	152	1.006222692969681	TruSeq Adapter, Index 1 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	140	0.9267840593141797	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	110	0.7281874751754269	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.5229710048987157	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	77	0.5097312326227988	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	77	0.5097312326227988	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	43	0.2846551039322124	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.2714153316562955	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.21183635641466966	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	31	0.20521647027671122	RNA PCR Primer, Index 1 (95% over 22bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.19859658413875278	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	23	0.15225738117304383	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	20	0.13239772275916853	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA	20	0.13239772275916853	No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA	19	0.12577783662121012	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	18	0.11915795048325169	No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC	18	0.11915795048325169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.006619886137958427	0.0
20	0.0	0.0	0.0	0.006619886137958427	0.0
21	0.0	0.0	0.0	0.006619886137958427	0.0
22	0.0	0.0	0.0	0.006619886137958427	0.0
23	0.0	0.0	0.0	0.01985965841387528	0.0
24	0.0	0.0	0.0	0.03309943068979213	0.0
25	0.0	0.0	0.0	0.03971931682775056	0.0
26	0.0	0.0	0.0	0.04633920296570899	0.0
27	0.0	0.0	0.0	0.07281874751754269	0.0
28	0.0	0.0	0.0	0.13239772275916853	0.0
29	0.0	0.0	0.0	0.20521647027671125	0.0
30	0.0	0.0	0.0	0.3839533960015888	0.0
31	0.0	0.0	0.0	0.7480471335893023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	130	0.0	22.76923	1
GTATCAA	170	6.002665E-11	16.32353	2
TATCAAC	200	6.9485395E-10	13.874999	3
CAACGCA	215	1.3278623E-10	13.767442	6
CTGTCTC	135	5.6031604E-6	13.703704	37
ATCAACG	220	1.9099389E-10	13.454545	4
TCAACGC	225	2.746674E-10	13.155556	5
AACGCAG	235	5.475158E-10	12.595744	7
CGCAGAG	290	1.2332748E-9	10.844828	9
AGTACAT	175	8.0441496E-5	10.571429	14
GAGTACA	175	8.0441496E-5	10.571429	13
ACATGGG	180	1.07084445E-4	10.277779	17
GTACATG	190	1.8507887E-4	9.736842	15
TACATGG	190	1.8507887E-4	9.736842	16
ACGCAGA	305	3.288551E-8	9.704918	8
CAGAGTA	305	3.288551E-8	9.704918	11
AGAGTAC	305	3.288551E-8	9.704918	12
GCAGAGT	320	6.919436E-8	9.25	10
>>END_MODULE