FastQCFastQC Report
Fri 10 Feb 2017
ERR1632234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96496
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5610.5813712485491627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.4601226993865031No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3010.3119300281876969No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT2060.21348035151716135Illumina PCR Primer Index 11 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.18342729232299784No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC1330.1378295473387498No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.12539379870668213No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT1140.11813961200464267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAA303.5752976E-430.83333430
TGAAGTT250.005471721329.65
ACACATA250.005471721329.66
TAGCAGC250.005471721329.66
GGTCTCC250.005471721329.622
TTATACA358.8103727E-426.4285722
TCTTATA1200.024.66666837
GGTATCA3200.024.281251
TATACAC400.001918458123.1253
TACACAT806.825139E-720.81255
ATACACA555.096299E-420.1818184
GTATCAA4050.019.1851842
CTCTTAT1601.8189894E-1218.536
TATGCCG500.006989393418.533
ATCAACG4700.016.5319144
CAACGCA4950.016.0707076
TATCAAC5100.015.2352943
TCAACGC5150.015.0873795
AACGCAG5550.014.3333337
TCTCTTA2202.6921043E-1013.45454535
Produced by FastQC (version 0.11.5)