##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96496
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240092853589786	31.0	31.0	34.0	30.0	34.0
2	31.434670867186206	31.0	31.0	34.0	30.0	34.0
3	31.47425800033162	31.0	31.0	34.0	30.0	34.0
4	35.30312137290665	37.0	35.0	37.0	33.0	37.0
5	34.38132150555463	35.0	35.0	37.0	30.0	37.0
6	34.361538302105785	35.0	35.0	37.0	30.0	37.0
7	34.3597972972973	35.0	35.0	37.0	30.0	37.0
8	34.29053017741668	35.0	35.0	37.0	30.0	37.0
9	35.82066614160173	37.0	35.0	39.0	30.0	39.0
10	35.51486071961532	37.0	35.0	39.0	30.0	39.0
11	35.73178162825402	37.0	35.0	39.0	30.0	39.0
12	35.651394876471564	37.0	35.0	39.0	30.0	39.0
13	35.75274622782292	37.0	35.0	39.0	30.0	39.0
14	36.598553307909135	38.0	36.0	40.0	31.0	41.0
15	36.55902835350688	38.0	36.0	40.0	31.0	41.0
16	36.490331205438565	38.0	35.0	40.0	31.0	41.0
17	36.49394793566573	38.0	35.0	40.0	31.0	41.0
18	36.51314044105455	38.0	36.0	40.0	31.0	41.0
19	36.51959666721937	38.0	35.0	40.0	30.0	41.0
20	36.47022674515006	38.0	35.0	40.0	30.0	41.0
21	36.471439230641685	38.0	35.0	40.0	30.0	41.0
22	36.4123072458962	38.0	35.0	40.0	30.0	41.0
23	36.33891560271928	38.0	35.0	40.0	30.0	41.0
24	36.2248590615155	38.0	35.0	40.0	30.0	41.0
25	36.12382896700382	38.0	35.0	40.0	30.0	41.0
26	35.916960288509365	38.0	34.0	40.0	29.0	41.0
27	35.791939562261646	38.0	34.0	40.0	29.0	41.0
28	35.67837008787929	38.0	34.0	40.0	29.0	41.0
29	35.545411208754764	38.0	34.0	40.0	28.0	41.0
30	35.39279348366772	38.0	34.0	40.0	27.0	41.0
31	35.24835226330625	38.0	34.0	40.0	27.0	41.0
32	35.0442609020063	38.0	33.0	40.0	27.0	41.0
33	34.909177582490464	37.0	33.0	40.0	26.0	40.0
34	34.771389487647156	37.0	33.0	40.0	26.0	40.0
35	34.71857900845631	37.0	33.0	39.0	26.0	40.0
36	34.623455894544854	37.0	33.0	39.0	25.0	40.0
37	34.51668462941469	37.0	33.0	39.0	25.0	40.0
38	34.418058779638535	37.0	33.0	39.0	25.0	40.0
39	34.29482051069474	37.0	33.0	39.0	24.0	40.0
40	34.151861217045266	37.0	32.0	39.0	24.0	40.0
41	34.05672774000995	37.0	32.0	39.0	24.0	40.0
42	33.91598615486652	37.0	32.0	39.0	24.0	40.0
43	32.91613123860056	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	7.0
18	12.0
19	30.0
20	61.0
21	104.0
22	183.0
23	274.0
24	451.0
25	696.0
26	976.0
27	1382.0
28	1900.0
29	2504.0
30	3290.0
31	4378.0
32	5513.0
33	7094.0
34	9237.0
35	11499.0
36	13540.0
37	14975.0
38	13193.0
39	5193.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.2317609020063	20.822624772011274	13.463770518985244	24.48184380699718
2	20.2941054551484	21.36979771182225	33.31226164815122	25.02383518487813
3	21.449593765544687	22.981263472061016	29.891394461946607	25.67774830044769
4	16.861838832697728	17.09915436909302	32.2604045763555	33.77860222185375
5	15.992372740838997	34.77657104957718	32.437613994362465	16.793442215221358
6	33.00758580666556	33.123652793898195	16.146783286353838	17.721978113082407
7	27.72861051235284	30.820966672193666	20.655778477864366	20.794644337589123
8	27.178328635383853	32.45419499253855	20.00911954899685	20.35835682308075
9	26.250829049908802	13.550820759409715	20.99465262808821	39.20369756259327
10	18.55517327143094	25.71505554634389	31.208547504559775	24.521223677665397
11	35.3133808655281	21.99262145581164	20.41742662908307	22.276571049577186
12	21.364616149892225	26.725460122699385	27.538965345713812	24.370958381694578
13	31.809608688443042	19.14483501906815	24.516042115735367	24.52951417675344
14	23.445531420991543	21.100356491460786	24.550240424473554	30.903871663074117
15	28.035358978610514	25.90470071298292	21.659965179903832	24.399975128502735
16	25.5140109434588	24.870460951749294	24.677706847952248	24.937821256839662
17	23.85072956391975	26.487108273918093	24.7606118388327	24.901550323329463
18	23.68906483170287	24.03830210578677	26.440474216547837	25.832158845962528
19	25.153374233128833	25.34301939976787	26.925468413198473	22.578137953904825
20	25.18239097993699	23.696319018404907	27.114077267451503	24.007212734206597
21	25.785524788592273	23.228942132316366	26.128544188360138	24.85698889073122
22	26.41353009451169	23.676629083070804	25.435251202122366	24.47458962029514
23	25.459086387000497	23.6942463936329	25.690184049079758	25.156483170286855
24	24.908804510031505	24.727449842480517	26.319225667385176	24.044519980102802
25	24.70775990714641	24.347123196816447	25.528519316862873	25.416597579174265
26	25.055960868844306	23.664193334438732	25.938899021721106	25.340946774995853
27	24.993782125683968	24.42277400099486	25.803142099154368	24.780301774166805
28	24.39168462941469	23.929489305256176	26.40627590780965	25.27255015751948
29	24.04037473055878	24.56578511026364	26.60732051069474	24.786519648482837
30	23.912908307080087	24.941966506383682	26.970029845796716	24.175095340739514
31	24.502570054717292	24.646617476372075	25.849776156524623	25.001036312386006
32	24.464226496435085	24.573039296965675	26.366896037141434	24.5958381694578
33	24.080790913612997	24.38961200464268	26.628046758414857	24.901550323329463
34	24.464226496435085	23.948142928204277	26.776239429613664	24.811391145746974
35	24.224838335267783	24.14711490631736	26.77209418006964	24.855952578345217
36	24.131570220527276	24.022757419996683	26.45601890233792	25.389653457138117
37	24.068355164980932	24.09322666224507	26.610429447852763	25.22798872492124
38	23.818603879953574	23.44656773337755	27.51720278560769	25.217625601061183
39	24.054883103962858	22.936702039462777	28.248839330127673	24.759575526446692
40	23.143964516663903	22.725294312717626	28.598076604211574	25.532664566406897
41	23.00302603216714	23.097330459293648	28.38045100315039	25.519192505388826
42	22.965718786270934	23.192671198806167	28.60636710329962	25.235242911623278
43	22.57710164151882	22.998880782623115	28.66854584645996	25.75547172939811
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	6.0
2	9.0
3	27.0
4	45.0
5	45.0
6	76.5
7	108.0
8	101.0
9	94.0
10	149.0
11	204.0
12	204.0
13	397.0
14	590.0
15	778.0
16	966.0
17	857.5
18	749.0
19	749.0
20	875.0
21	1001.0
22	725.5
23	450.0
24	473.5
25	497.0
26	497.0
27	597.5
28	698.0
29	812.5
30	927.0
31	1121.0
32	1315.0
33	1315.0
34	1626.0
35	1937.0
36	2270.5
37	2604.0
38	3146.5
39	3689.0
40	3689.0
41	4209.0
42	4729.0
43	5542.0
44	6355.0
45	7045.5
46	7736.0
47	7736.0
48	8696.5
49	9657.0
50	9749.5
51	9842.0
52	9748.5
53	9655.0
54	9655.0
55	8921.0
56	8187.0
57	7840.5
58	7494.0
59	6508.0
60	5522.0
61	5522.0
62	4572.5
63	3623.0
64	3126.0
65	2629.0
66	2277.0
67	1925.0
68	1925.0
69	1585.5
70	1246.0
71	1037.0
72	828.0
73	688.0
74	548.0
75	548.0
76	417.0
77	286.0
78	235.0
79	184.0
80	128.5
81	73.0
82	73.0
83	59.5
84	46.0
85	36.0
86	26.0
87	19.5
88	13.0
89	13.0
90	9.5
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	96496.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.17845299287018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36565032506559	72.45378046758415
2	6.096757099096537	9.776571049577184
3	1.5044785379157026	3.6188028519316866
4	0.6785663508640412	2.176256010611839
5	0.3567320244542388	1.4301110926877798
6	0.26237898900076256	1.2622284861548665
7	0.1835360415670359	1.0300945116896036
8	0.10469309413330921	0.6715304261316531
9	0.0995230647933927	0.7181644835019068
>10	0.3308818777546563	4.377383518487813
>50	0.00646253667489563	0.3533825236279224
>100	0.009047551344853882	1.5503233294644339
>500	0.001292507334979126	0.5813712485491627
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	561	0.5813712485491627	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	444	0.4601226993865031	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	301	0.3119300281876969	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	206	0.21348035151716135	Illumina PCR Primer Index 11 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	177	0.18342729232299784	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	133	0.1378295473387498	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.12539379870668213	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	114	0.11813961200464267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0010363123860056376	0.0
17	0.0	0.0	0.0	0.0010363123860056376	0.0
18	0.0	0.0010363123860056376	0.0	0.0010363123860056376	0.0
19	0.0	0.0010363123860056376	0.0	0.0010363123860056376	0.0
20	0.0	0.0010363123860056376	0.0	0.0010363123860056376	0.0
21	0.0	0.0010363123860056376	0.0	0.0010363123860056376	0.0
22	0.0	0.0010363123860056376	0.0	0.0031089371580169125	0.0
23	0.0	0.0010363123860056376	0.0	0.0031089371580169125	0.0
24	0.0	0.0010363123860056376	0.0	0.006217874316033825	0.0
25	0.0	0.0010363123860056376	0.0	0.006217874316033825	0.0
26	0.0	0.0010363123860056376	0.0	0.0082904990880451	0.0
27	0.0	0.0010363123860056376	0.0	0.0248714972641353	0.0
28	0.0	0.0010363123860056376	0.0	0.05492455645829879	0.0
29	0.0	0.0010363123860056376	0.0	0.11399436246062013	0.0
30	0.0	0.0010363123860056376	0.0	0.18031835516498093	0.0
31	0.0	0.0010363123860056376	0.0	0.42903332780633396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTGAA	30	3.5752976E-4	30.833334	30
TGAAGTT	25	0.0054717213	29.6	5
ACACATA	25	0.0054717213	29.6	6
TAGCAGC	25	0.0054717213	29.6	6
GGTCTCC	25	0.0054717213	29.6	22
TTATACA	35	8.8103727E-4	26.428572	2
TCTTATA	120	0.0	24.666668	37
GGTATCA	320	0.0	24.28125	1
TATACAC	40	0.0019184581	23.125	3
TACACAT	80	6.825139E-7	20.8125	5
ATACACA	55	5.096299E-4	20.181818	4
GTATCAA	405	0.0	19.185184	2
CTCTTAT	160	1.8189894E-12	18.5	36
TATGCCG	50	0.0069893934	18.5	33
ATCAACG	470	0.0	16.531914	4
CAACGCA	495	0.0	16.070707	6
TATCAAC	510	0.0	15.235294	3
TCAACGC	515	0.0	15.087379	5
AACGCAG	555	0.0	14.333333	7
TCTCTTA	220	2.6921043E-10	13.454545	35
>>END_MODULE