FastQCFastQC Report
Fri 10 Feb 2017
ERR1632233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293862
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27860.9480640572785866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19220.6540484989552919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18940.6445202169725925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.2742784027877031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGT250.00548977129.5999981
GTCTTAC250.00548977129.5999981
CGAAGGA250.00548977129.59999814
GCGTCAG1650.028.0303024
GGTATCA5250.027.8380971
CGTCAGA1700.027.2058835
ATGTGTA1750.026.42857211
GCGTTAT509.061056E-625.8999981
AGCGTCA1800.025.6944453
ATAAGAG1800.025.69444517
GTATAAG1800.025.69444515
TATAAGA1800.025.69444516
CAGCGTC1900.025.315792
GATGTGT1900.024.34210610
GCAGCGT1850.023.01
GTCAGAT2100.022.904766
TGTATAA2050.022.56097414
TCAGATG2100.022.023817
TAAGAGA2200.021.86363618
GTATTAG603.71415E-521.5833321
Produced by FastQC (version 0.11.5)