##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632233.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	293862
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.236121036404842	31.0	30.0	31.0	27.0	34.0
2	30.37412458909284	31.0	30.0	31.0	27.0	34.0
3	30.4611790568362	31.0	30.0	33.0	27.0	34.0
4	34.23356881801662	35.0	35.0	37.0	30.0	37.0
5	32.576093540505404	35.0	32.0	37.0	25.0	37.0
6	32.573085325765156	35.0	32.0	37.0	25.0	37.0
7	32.59223036663468	35.0	32.0	36.0	26.0	37.0
8	32.49517120280948	35.0	32.0	36.0	25.0	37.0
9	33.8019614649053	35.0	32.0	38.0	27.0	39.0
10	33.33515731874145	35.0	32.0	38.0	25.0	39.0
11	33.652047559738925	35.0	32.0	38.0	26.0	39.0
12	33.509528281982696	35.0	32.0	38.0	26.0	39.0
13	33.67908065690698	35.0	32.0	38.0	26.0	39.0
14	34.28233320402094	36.0	32.0	39.0	26.0	40.0
15	34.26679189551558	36.0	32.0	39.0	26.0	40.0
16	34.23953080017151	36.0	32.0	39.0	26.0	40.0
17	34.21217442200761	36.0	32.0	39.0	26.0	40.0
18	34.22850181377653	36.0	32.0	39.0	25.0	40.0
19	34.29791194506265	37.0	32.0	39.0	25.0	40.0
20	34.24158958967134	37.0	32.0	39.0	25.0	40.0
21	34.20058054460937	37.0	32.0	39.0	25.0	40.0
22	34.12936003974655	36.0	32.0	39.0	25.0	40.0
23	34.005175898891316	36.0	32.0	39.0	25.0	40.0
24	33.87685035833146	36.0	32.0	39.0	25.0	40.0
25	33.7712667850896	36.0	31.0	39.0	24.0	40.0
26	33.53095330461237	36.0	31.0	39.0	24.0	40.0
27	33.33858069433952	36.0	31.0	39.0	24.0	40.0
28	33.20607972449653	36.0	31.0	39.0	23.0	40.0
29	33.01970312595708	36.0	30.0	39.0	22.0	40.0
30	32.83861812687588	36.0	30.0	38.0	21.0	40.0
31	32.65771348456078	35.0	30.0	38.0	21.0	40.0
32	32.43942054433714	35.0	30.0	38.0	19.0	40.0
33	32.270838012400375	35.0	30.0	38.0	19.0	40.0
34	32.13639395362449	35.0	30.0	38.0	19.0	40.0
35	32.04812122697048	35.0	30.0	38.0	18.0	40.0
36	31.909549380321376	35.0	29.0	38.0	18.0	40.0
37	31.8102612791038	35.0	29.0	38.0	18.0	40.0
38	31.675772981875845	35.0	29.0	38.0	18.0	40.0
39	31.561545895692536	35.0	29.0	38.0	18.0	40.0
40	31.375863500554683	34.0	28.0	38.0	16.0	40.0
41	31.2929810591366	34.0	28.0	38.0	16.0	40.0
42	31.15167323437532	34.0	27.0	38.0	15.0	40.0
43	30.244094166649653	33.0	26.0	38.0	15.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	4.0
13	19.0
14	38.0
15	74.0
16	131.0
17	240.0
18	469.0
19	716.0
20	1165.0
21	1612.0
22	2522.0
23	3477.0
24	4680.0
25	6419.0
26	8400.0
27	10503.0
28	13202.0
29	16022.0
30	18504.0
31	21463.0
32	23850.0
33	25928.0
34	28213.0
35	29166.0
36	28343.0
37	24366.0
38	16994.0
39	7337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.00483220014837	22.06579959300624	14.778025059381614	23.151343147463777
2	18.73226208220185	23.864262817240743	34.28514064424798	23.118334456309427
3	20.19995780332265	25.320388481668267	31.229624789867355	23.250028925141734
4	15.96769912407865	17.71171502269773	34.57745472364579	31.74313112957783
5	13.855483185985259	37.75275469438035	33.319381206144385	15.072380913490004
6	32.056543547651614	37.201135226739076	16.013639055066665	14.728682170542637
7	27.041944858470984	32.270249300692164	22.158360046552463	18.529445794284392
8	26.867373120716525	34.2735705875547	20.40992030272713	18.44913598900164
9	26.450851079758525	14.738891044095528	20.96494272821937	37.84531514792658
10	16.687424709557547	28.546732820167293	32.79770776759159	21.96813470268357
11	34.58528152670301	22.895100421286184	22.061716043585083	20.457902008425723
12	20.825421456329842	27.478544350749672	30.49492618984421	21.201108003076275
13	30.607223798926025	20.49329277007575	25.88323771021772	23.016245720780503
14	21.913006785497956	21.66152820031171	27.931818336498083	28.493646677692254
15	24.659534067011045	28.565108792562494	24.128672642260653	22.646684498165808
16	22.781101333278887	27.55817356446223	26.228978227875672	23.431746874383215
17	21.607080874696287	28.052963635992406	27.416270222077028	22.92368526723428
18	21.609122649406864	25.464333598764043	30.09950248756219	22.8270412642669
19	22.637156216183108	26.535925026032626	29.619004839006063	21.2079139187782
20	23.57603228726409	25.877792977656178	29.126937133756662	21.419237601323072
21	24.501296526941218	25.897530133191772	28.00498193029381	21.5961914095732
22	23.504229876608747	26.509041659010013	28.3942803084441	21.59244815593714
23	23.376618957197596	25.8475066527826	28.53380158033363	22.242072809686178
24	22.719848091961534	26.67374481899667	28.52291211521054	22.083494973831254
25	22.652469526512444	25.60419516643867	28.63827238635822	23.105062920690663
26	22.151213835065438	26.51618787049704	28.789704010726126	22.5428942837114
27	23.00433536830213	25.93019852856103	28.54809400330767	22.51737209982917
28	22.646003906595613	26.131653633338097	28.938753564598347	22.28358889546794
29	22.14270644043803	26.31983720249641	29.21235137581586	22.3251049812497
30	22.283248599682846	26.778896216591463	28.768265376265052	22.169589807460643
31	22.659275442214373	26.422266233810426	28.584165356527897	22.334292967447304
32	21.842905853768098	26.36441595034404	29.051051173680165	22.7416270222077
33	21.99978221069754	26.105110562100574	29.32464898489767	22.570458242304213
34	22.460542703718072	25.65523953420313	29.349150281424613	22.535067480654185
35	22.360836038684827	25.54260162933622	29.64588820602868	22.450674125950275
36	22.118885735481282	25.990430882523093	29.00511124269215	22.885572139303484
37	22.330209418026147	25.30303339662835	29.271562842422632	23.095194342922866
38	22.047763916396132	25.182908984489316	29.817737577502363	22.951589521612185
39	21.92661861690181	24.753115407912556	30.27135185903587	23.048914116149756
40	21.5019294771015	24.58228692379416	30.93492863997386	22.980854959130475
41	20.953712967311187	24.426771750005106	31.325247905479443	23.294267377204267
42	21.10004015490264	24.51048451313882	31.15067616772499	23.23879916423355
43	20.665482437334532	23.925856354343196	31.2915586227549	24.117102585567377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	159.0
1	151.5
2	144.0
3	243.0
4	342.0
5	342.0
6	465.0
7	588.0
8	597.5
9	607.0
10	801.0
11	995.0
12	995.0
13	1721.0
14	2447.0
15	3827.5
16	5208.0
17	4799.5
18	4391.0
19	4391.0
20	4622.5
21	4854.0
22	4381.0
23	3908.0
24	4469.0
25	5030.0
26	5030.0
27	5790.5
28	6551.0
29	7529.0
30	8507.0
31	9649.0
32	10791.0
33	10791.0
34	11758.0
35	12725.0
36	13825.0
37	14925.0
38	15754.5
39	16584.0
40	16584.0
41	17215.0
42	17846.0
43	18005.0
44	18164.0
45	18302.0
46	18440.0
47	18440.0
48	18795.5
49	19151.0
50	19040.5
51	18930.0
52	19204.5
53	19479.0
54	19479.0
55	18842.5
56	18206.0
57	17467.0
58	16728.0
59	15228.0
60	13728.0
61	13728.0
62	12343.0
63	10958.0
64	9434.0
65	7910.0
66	6691.0
67	5472.0
68	5472.0
69	4597.5
70	3723.0
71	3181.5
72	2640.0
73	2067.5
74	1495.0
75	1495.0
76	1186.0
77	877.0
78	711.5
79	546.0
80	452.5
81	359.0
82	359.0
83	284.5
84	210.0
85	162.5
86	115.0
87	94.0
88	73.0
89	73.0
90	58.0
91	43.0
92	25.5
93	8.0
94	6.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	293862.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.31576417208936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.350639877917	80.3956206560427
2	2.7084779669406145	4.567347790517298
3	0.6505290668466059	1.645495661619833
4	0.3359320848745272	1.1329748178447576
5	0.1894256949454496	0.7985786111577334
6	0.14265076743329544	0.7216625079523982
7	0.1108112784218226	0.654019633331549
8	0.08422592981792634	0.5681258908562582
9	0.06145621581370126	0.46635550195113584
>10	0.3480385558736188	5.366458958810287
>50	0.013359420836602824	0.7406997622767368
>100	0.002833816541091636	0.4140409270231306
>500	4.048309344416623E-4	0.2751170545594751
>1k	0.001214492803324987	2.2535022260566433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2786	0.9480640572785866	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1922	0.6540484989552919	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.6445202169725925	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	806	0.2742784027877031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.402957850964058E-4	0.0
5	0.0	3.402957850964058E-4	0.0	3.402957850964058E-4	0.0
6	0.0	3.402957850964058E-4	0.0	3.402957850964058E-4	0.0
7	0.0	3.402957850964058E-4	0.0	3.402957850964058E-4	0.0
8	0.0	3.402957850964058E-4	0.0	3.402957850964058E-4	0.0
9	0.0	3.402957850964058E-4	0.0	6.805915701928116E-4	0.0
10	0.0	3.402957850964058E-4	0.0	0.0010208873552892174	0.0
11	0.0	3.402957850964058E-4	0.0	0.0010208873552892174	0.0
12	0.0	3.402957850964058E-4	0.0	0.0010208873552892174	0.0
13	0.0	3.402957850964058E-4	0.0	0.0010208873552892174	0.0
14	0.0	3.402957850964058E-4	0.0	0.0010208873552892174	0.0
15	0.0	3.402957850964058E-4	0.0	0.0010208873552892174	0.0
16	0.0	3.402957850964058E-4	0.0	0.0013611831403856232	0.0
17	0.0	3.402957850964058E-4	0.0	0.0013611831403856232	0.0
18	0.0	3.402957850964058E-4	0.0	0.0013611831403856232	0.0
19	0.0	3.402957850964058E-4	0.0	0.0020417747105784347	0.0
20	0.0	3.402957850964058E-4	0.0	0.0020417747105784347	0.0
21	0.0	3.402957850964058E-4	0.0	0.0023820704956748406	0.0
22	0.0	3.402957850964058E-4	0.0	0.0034029578509640578	0.0
23	0.0	3.402957850964058E-4	0.0	0.0040835494211568695	0.0
24	0.0	3.402957850964058E-4	0.0	0.007146211487024522	0.0
25	0.0	3.402957850964058E-4	0.0	0.007826803057217334	0.0
26	0.0	3.402957850964058E-4	0.0	0.009187986197602957	0.0
27	0.0	3.402957850964058E-4	0.0	0.022459521816362783	0.0
28	0.0	3.402957850964058E-4	0.0	0.07758743900198052	0.0
29	0.0	3.402957850964058E-4	0.0	0.15551517378905744	0.0
30	0.0	3.402957850964058E-4	0.0	0.2684933744410642	0.0
31	0.0	3.402957850964058E-4	0.0	0.6373740054855681	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTGT	25	0.005489771	29.599998	1
GTCTTAC	25	0.005489771	29.599998	1
CGAAGGA	25	0.005489771	29.599998	14
GCGTCAG	165	0.0	28.030302	4
GGTATCA	525	0.0	27.838097	1
CGTCAGA	170	0.0	27.205883	5
ATGTGTA	175	0.0	26.428572	11
GCGTTAT	50	9.061056E-6	25.899998	1
AGCGTCA	180	0.0	25.694445	3
ATAAGAG	180	0.0	25.694445	17
GTATAAG	180	0.0	25.694445	15
TATAAGA	180	0.0	25.694445	16
CAGCGTC	190	0.0	25.31579	2
GATGTGT	190	0.0	24.342106	10
GCAGCGT	185	0.0	23.0	1
GTCAGAT	210	0.0	22.90476	6
TGTATAA	205	0.0	22.560974	14
TCAGATG	210	0.0	22.02381	7
TAAGAGA	220	0.0	21.863636	18
GTATTAG	60	3.71415E-5	21.583332	1
>>END_MODULE