FastQCFastQC Report
Fri 10 Feb 2017
ERR1632232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150261
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC200.001836848437.03
GCAAGTT250.005481338629.59999826
GGTATCA1053.8198777E-1122.904761
GAATAGG502.6869844E-422.1999991
GCCAAAA502.6869844E-422.19999911
AGGTGTC603.698612E-521.5833327
GGTGTCA450.003810580420.5555558
GTTCAGC450.003810580420.5555551
ATCAACG1306.8030204E-1018.54
TACACCA500.007007151418.4999985
GTATCAA1351.1241355E-917.8148142
TCAACGC1351.1241355E-917.8148145
TATCAAC1606.1299943E-1016.18753
CTCTTAT3100.016.11290437
CAACGCA1504.5620254E-916.0333336
CTTAGGA700.002578457615.8571422
AACGCAG1557.041308E-915.51612957
CAGGTGT750.004082367314.86
AATACCA750.004082367314.821
CTTATAC1203.2629945E-513.87499937
Produced by FastQC (version 0.11.5)