##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150261
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.797711981152794	34.0	31.0	34.0	31.0	34.0
2	32.964934347568565	34.0	33.0	34.0	31.0	34.0
3	32.96510737982577	34.0	33.0	34.0	31.0	34.0
4	36.397341958325846	37.0	37.0	37.0	35.0	37.0
5	36.31545777014661	37.0	37.0	37.0	35.0	37.0
6	36.34741549703516	37.0	37.0	37.0	35.0	37.0
7	36.33294068321121	37.0	37.0	37.0	35.0	37.0
8	36.32414265844098	37.0	37.0	37.0	35.0	37.0
9	38.109076872907806	39.0	39.0	39.0	37.0	39.0
10	38.046645503490595	39.0	38.0	39.0	37.0	39.0
11	38.14832191985944	39.0	39.0	39.0	37.0	39.0
12	38.116550535401736	39.0	38.0	39.0	37.0	39.0
13	38.136715448453025	39.0	39.0	39.0	37.0	39.0
14	39.558474920305336	41.0	39.0	41.0	37.0	41.0
15	39.58491558022374	41.0	39.0	41.0	37.0	41.0
16	39.4537371640013	41.0	39.0	41.0	37.0	41.0
17	39.51322698504602	41.0	39.0	41.0	37.0	41.0
18	39.530829689673304	41.0	39.0	41.0	37.0	41.0
19	39.589041734049424	41.0	39.0	41.0	37.0	41.0
20	39.5724040170104	41.0	39.0	41.0	37.0	41.0
21	39.541677481182745	41.0	39.0	41.0	37.0	41.0
22	39.52497986836238	41.0	39.0	41.0	37.0	41.0
23	39.49273597274077	41.0	39.0	41.0	37.0	41.0
24	39.44310233526996	41.0	39.0	41.0	37.0	41.0
25	39.381775710264144	41.0	39.0	41.0	36.0	41.0
26	39.29947890670234	40.0	39.0	41.0	36.0	41.0
27	39.22963377057254	40.0	39.0	41.0	36.0	41.0
28	39.169791229926595	40.0	39.0	41.0	36.0	41.0
29	39.03664290800673	40.0	39.0	41.0	36.0	41.0
30	38.99597367247656	40.0	39.0	41.0	35.0	41.0
31	38.94413720126979	40.0	39.0	41.0	35.0	41.0
32	38.89029754893153	40.0	38.0	41.0	35.0	41.0
33	38.765834115306035	40.0	38.0	41.0	35.0	41.0
34	38.765527981312516	40.0	38.0	41.0	35.0	41.0
35	38.75434078037548	40.0	38.0	41.0	35.0	41.0
36	38.734262383452794	40.0	38.0	41.0	35.0	41.0
37	38.69610877073892	40.0	38.0	41.0	35.0	41.0
38	38.623082503111256	40.0	38.0	41.0	35.0	41.0
39	38.444613039977106	40.0	38.0	41.0	35.0	41.0
40	38.46722702497654	40.0	38.0	41.0	34.0	41.0
41	38.47604501500722	40.0	38.0	41.0	35.0	41.0
42	38.366542216543216	40.0	38.0	41.0	34.0	41.0
43	37.52879323310773	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	6.0
21	21.0
22	42.0
23	55.0
24	99.0
25	121.0
26	222.0
27	352.0
28	424.0
29	580.0
30	860.0
31	1163.0
32	1640.0
33	2216.0
34	3393.0
35	5090.0
36	8285.0
37	14909.0
38	35875.0
39	74904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15731294214733	18.30082323423909	12.72519150012312	26.816672323490458
2	19.759618264220258	20.246105110441164	33.50237253844976	26.491904086888812
3	20.76120882996919	21.42937954625618	29.203186455567316	28.606225168207317
4	15.775883296397602	15.423829203851962	33.103067329513316	35.697220170237124
5	16.215784534909258	34.521931838600835	33.224189909557374	16.038093716932536
6	35.9401308390068	33.03784747872036	15.18957014794258	15.832451534330266
7	29.826102581508174	28.812532859491153	20.828425206806823	20.53293935219385
8	27.112823686784992	32.6318871829683	19.682419257159207	20.572869873087495
9	26.48791103479945	14.168014321746828	19.64781280571805	39.696261837735676
10	17.70519296424222	25.891615256121014	32.743027132788946	23.660164646847818
11	36.55572636945049	20.7033095746734	20.795815281410345	21.945148774465764
12	22.34445398340221	24.53065000232928	27.93472690851252	25.190169105755984
13	31.4958638635441	19.047523974950252	23.32408276265964	26.13252939884601
14	23.929695662879922	20.56954232967969	23.39728871763132	32.10347328980907
15	26.543148255368994	26.809351727993292	20.969513047297703	25.677986969340015
16	26.34016810749296	25.19416215784535	22.75706936596988	25.70860036869181
17	24.741616254384038	25.66534230439036	24.168613279560233	25.424428161665368
18	24.596535361803795	24.144654967024046	25.459034613106528	25.799775058065634
19	25.838374561596154	24.555939332228586	24.882038586193357	24.7236475199819
20	26.15249465929283	24.119365637124737	24.85075967816	24.877380025422433
21	26.2989065692362	24.436813278229213	24.011553230711893	25.252726921822692
22	26.37477455893412	24.20321973100139	23.967629657728885	25.454376052335604
23	25.679317986703136	24.155303105929015	24.134672336800634	26.030706570567215
24	25.747199872222332	24.571246031904487	24.020870352253745	25.660683743619433
25	25.94818349405368	23.895754720120323	24.195899135504224	25.96016265032177
26	25.831053966098988	24.470088712307252	24.107386480856643	25.591470840737117
27	25.703276299239324	24.160627175381503	24.104724446130398	26.031372079248772
28	25.022460918002675	24.20920930913544	24.921969107087	25.84636066577489
29	24.979202853701228	24.315690698185158	24.764909058238665	25.940197389874953
30	25.040429652404818	24.95457903248348	24.651772582373336	25.35321873273837
31	25.705938333965566	24.426830648005804	24.299718489827697	25.56751252820093
32	24.963896154025328	24.164620227470866	24.701020224808833	26.17046339369497
33	24.687044542496057	24.01687730016438	24.90266935532174	26.39340880201782
34	25.357877293509297	23.704753728512387	24.797518983635143	26.13984999434318
35	24.962565136662207	23.656837103440015	25.299312529531946	26.08128523036583
36	24.686379033814497	23.762652983808174	25.54022667225694	26.010741310120387
37	25.258050991275184	22.741097157612423	25.86033634808766	26.140515503024737
38	24.39887928338025	22.91878797558914	26.953767111891974	25.728565629138632
39	23.97428474454449	22.49286241939026	27.75903261658048	25.77382021948476
40	23.50310459799948	22.32448872295539	28.04453584097005	26.127870838075083
41	22.552092692049168	22.572057952495992	28.853128889066358	26.02272046638848
42	22.891502119645153	22.142804852889306	28.72202367879889	26.243669348666653
43	22.00437904712467	22.17142172619642	29.301016231756744	26.523182994922166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	3.0
2	1.0
3	10.5
4	20.0
5	20.0
6	21.5
7	23.0
8	28.0
9	33.0
10	50.5
11	68.0
12	68.0
13	101.0
14	134.0
15	223.5
16	313.0
17	327.0
18	341.0
19	341.0
20	439.0
21	537.0
22	665.0
23	793.0
24	1014.5
25	1236.0
26	1236.0
27	1464.0
28	1692.0
29	2215.0
30	2738.0
31	3110.5
32	3483.0
33	3483.0
34	3959.5
35	4436.0
36	5148.0
37	5860.0
38	6515.5
39	7171.0
40	7171.0
41	7729.5
42	8288.0
43	8549.5
44	8811.0
45	9288.5
46	9766.0
47	9766.0
48	10155.5
49	10545.0
50	11131.5
51	11718.0
52	12329.5
53	12941.0
54	12941.0
55	12152.5
56	11364.0
57	11037.0
58	10710.0
59	9948.5
60	9187.0
61	9187.0
62	8476.0
63	7765.0
64	6929.0
65	6093.0
66	5300.0
67	4507.0
68	4507.0
69	3897.5
70	3288.0
71	2880.0
72	2472.0
73	2026.5
74	1581.0
75	1581.0
76	1228.5
77	876.0
78	746.0
79	616.0
80	507.0
81	398.0
82	398.0
83	320.5
84	243.0
85	178.5
86	114.0
87	85.5
88	57.0
89	57.0
90	41.5
91	26.0
92	16.5
93	7.0
94	4.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	150261.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.32928322509576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.08693591012023	70.43687465099832
2	4.921474268536608	7.611481551933766
3	1.3793891620195011	3.2000152556230046
4	0.6705939346406268	2.074261932034255
5	0.4063583748147909	1.571170092847129
6	0.28649580109910977	1.3292708967596254
7	0.19365268271844435	1.0482516204463932
8	0.17568381218018508	1.0868402616116848
9	0.13375911103873653	0.930914656330634
>10	0.7246946996332126	9.59860758486076
>50	0.019964041141493295	1.0266303195124191
>100	9.982020570746646E-4	0.08568117704201393
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	6.65508681560751E-4	0.0
18	0.0	0.0	0.0	6.65508681560751E-4	0.0
19	0.0	0.0	0.0	0.001331017363121502	0.0
20	0.0	0.0	0.0	0.001996526044682253	0.0
21	0.0	0.0	0.0	0.001996526044682253	0.0
22	0.0	0.0	0.0	0.003327543407803755	0.0
23	0.0	0.0	0.0	0.003993052089364506	0.0
24	0.0	0.0	0.0	0.00665508681560751	0.0
25	0.0	0.0	0.0	0.007320595497168261	0.0
26	0.0	0.0	0.0	0.009982630223411264	0.0
27	0.0	0.0	0.0	0.033940942759598296	0.0
28	0.0	0.0	0.0	0.10847791509440241	0.0
29	0.0	0.0	0.0	0.19699056974198229	0.0
30	0.0	0.0	0.0	0.3267647626463287	0.0
31	0.0	0.0	0.0	0.654860542655779	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAC	20	0.0018368484	37.0	3
GCAAGTT	25	0.0054813386	29.599998	26
GGTATCA	105	3.8198777E-11	22.90476	1
GAATAGG	50	2.6869844E-4	22.199999	1
GCCAAAA	50	2.6869844E-4	22.199999	11
AGGTGTC	60	3.698612E-5	21.583332	7
GGTGTCA	45	0.0038105804	20.555555	8
GTTCAGC	45	0.0038105804	20.555555	1
ATCAACG	130	6.8030204E-10	18.5	4
TACACCA	50	0.0070071514	18.499998	5
GTATCAA	135	1.1241355E-9	17.814814	2
TCAACGC	135	1.1241355E-9	17.814814	5
TATCAAC	160	6.1299943E-10	16.1875	3
CTCTTAT	310	0.0	16.112904	37
CAACGCA	150	4.5620254E-9	16.033333	6
CTTAGGA	70	0.0025784576	15.857142	2
AACGCAG	155	7.041308E-9	15.5161295	7
CAGGTGT	75	0.0040823673	14.8	6
AATACCA	75	0.0040823673	14.8	21
CTTATAC	120	3.2629945E-5	13.874999	37
>>END_MODULE