ERR1632230.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632230.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29336
Sequences flagged as poor quality	0
Sequence length	43
%GC	52

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	261	0.8896918461958003	RNA PCR Primer, Index 26 (100% over 22bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	108	0.36814835014998637	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	106	0.36133078811017183	No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGG	101	0.34428688301063537	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	85	0.2897463866921189	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	66	0.22497954731388056	RNA PCR Primer, Index 26 (100% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	60	0.20452686119443686	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.18748295609490045	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.1636214889555495	No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCC	45	0.15339514589582764	No Hit
CCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC	42	0.1431688028361058	No Hit
TGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTG	34	0.11589855467684755	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.11248977365694028	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	32	0.109080992637033	No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACA	31	0.10567221161712573	No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGG	31	0.10567221161712573	No Hit
CCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACG	31	0.10567221161712573	No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT	30	0.10226343059721843	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTAAC	30	8.038758E-6	37.0	2
CTAACAC	30	8.038758E-6	37.0	4
GCCGTCT	35	2.3104185E-5	31.714287	36
AAGGTTG	25	0.005410449	29.6	5
TATACAC	40	5.747939E-5	27.75	3
CCTAACA	40	5.747939E-5	27.75	3
CCGTCTT	40	5.747939E-5	27.75	37
TTATACA	40	5.747939E-5	27.75	2
ATGCCGT	40	5.747939E-5	27.75	34
GGTATCA	65	8.756615E-8	25.615385	1
TGCCGTC	45	1.2807398E-4	24.666668	35
TATGCCG	45	1.2807398E-4	24.666668	33
GTCCTAA	45	1.2807398E-4	24.666668	1
CGTATGC	45	1.2807398E-4	24.666668	31
TCTTATA	40	0.0018868772	23.125	37
ACACGTG	50	2.6160627E-4	22.2	7
GTATGCC	50	2.6160627E-4	22.2	32
CACGTGC	50	2.6160627E-4	22.2	8
CGCTCGT	50	2.6160627E-4	22.2	14
ACACATC	50	2.6160627E-4	22.2	6