##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632230.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29336
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.661644395964004	34.0	31.0	34.0	31.0	34.0
2	32.83341287155713	34.0	31.0	34.0	31.0	34.0
3	32.8162326152168	34.0	31.0	34.0	31.0	34.0
4	36.30593809653668	37.0	37.0	37.0	35.0	37.0
5	36.205447232069815	37.0	35.0	37.0	35.0	37.0
6	36.28548541041724	37.0	37.0	37.0	35.0	37.0
7	36.25443141532588	37.0	36.0	37.0	35.0	37.0
8	36.251193073356966	37.0	36.0	37.0	35.0	37.0
9	38.02287292064358	39.0	38.0	39.0	35.0	39.0
10	37.98009271884374	39.0	38.0	39.0	35.0	39.0
11	38.06466457594764	39.0	38.0	39.0	35.0	39.0
12	38.02420234524134	39.0	38.0	39.0	35.0	39.0
13	38.069573220616306	39.0	38.0	39.0	35.0	39.0
14	39.419007362967	40.0	39.0	41.0	37.0	41.0
15	39.42367739296428	40.0	39.0	41.0	37.0	41.0
16	39.29097354785929	40.0	39.0	41.0	36.0	41.0
17	39.30839241887101	40.0	39.0	41.0	36.0	41.0
18	39.38134033269703	40.0	39.0	41.0	37.0	41.0
19	39.41106490319062	40.0	39.0	41.0	37.0	41.0
20	39.36320561767112	40.0	39.0	41.0	37.0	41.0
21	39.350968093809655	40.0	39.0	41.0	36.0	41.0
22	39.31950504499591	40.0	39.0	41.0	36.0	41.0
23	39.27314562312517	40.0	39.0	41.0	36.0	41.0
24	39.16024679574584	40.0	39.0	41.0	36.0	41.0
25	39.08228797382056	40.0	39.0	41.0	36.0	41.0
26	38.95442459776384	40.0	39.0	41.0	35.0	41.0
27	38.9078606490319	40.0	39.0	41.0	35.0	41.0
28	38.800859012817014	40.0	38.0	41.0	35.0	41.0
29	38.68161985274066	40.0	38.0	41.0	35.0	41.0
30	38.59432097082083	40.0	38.0	41.0	35.0	41.0
31	38.49672757022089	40.0	38.0	41.0	34.0	41.0
32	38.33893509680938	40.0	38.0	41.0	34.0	41.0
33	38.21352604308699	40.0	38.0	41.0	33.0	41.0
34	38.18901690755386	40.0	38.0	41.0	33.0	41.0
35	38.07829970002727	40.0	38.0	41.0	33.0	41.0
36	38.069539132806106	40.0	38.0	41.0	33.0	41.0
37	37.9595377692937	40.0	37.0	41.0	33.0	41.0
38	37.89061221707117	40.0	37.0	41.0	33.0	41.0
39	37.633249250068175	40.0	37.0	41.0	33.0	41.0
40	37.561869375511314	40.0	37.0	41.0	33.0	41.0
41	37.578231524406874	40.0	36.0	41.0	33.0	41.0
42	37.43942596127625	40.0	36.0	41.0	32.0	41.0
43	36.50262476138533	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	5.0
21	10.0
22	11.0
23	23.0
24	36.0
25	54.0
26	81.0
27	93.0
28	136.0
29	179.0
30	224.0
31	351.0
32	430.0
33	572.0
34	789.0
35	1245.0
36	1882.0
37	3417.0
38	7552.0
39	12245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.645077720207254	18.560812653395146	13.604445050449959	26.189664575947642
2	21.052631578947366	18.963048813744205	33.14016907553859	26.844150531769838
3	19.423234251431687	22.54226888464685	30.12680665394055	27.907690209980913
4	15.38382874284156	15.772429779110988	32.50613580583583	36.337605672211616
5	15.100899918189256	34.796836651213525	33.50149986364876	16.60076356694846
6	33.76738478320153	33.6617125715844	15.090673575129534	17.480229070084537
7	28.38832833378784	29.64957731115353	20.923097900190893	21.03899645486774
8	28.62353422416144	30.40632669757295	18.448322879738203	22.521816198527407
9	26.087401145350427	13.406735751295336	18.976683937823836	41.52917916553041
10	19.658440141805293	23.626261248977364	30.212026179438233	26.503272429779113
11	36.37169348241069	21.171938914644123	18.761930733569677	23.69443686937551
12	21.356013089719116	26.612353422416145	25.569266430324518	26.462367057540227
13	32.424325061358054	17.657485683119717	22.293427870193618	27.624761385328604
14	24.590946277611124	19.941368966457595	22.55590400872648	32.9117807472048
15	29.516634851377148	24.91478047450232	18.946004908644667	26.62257976547587
16	28.40878101990728	22.44341423506954	22.460458140169077	26.687346604854106
17	23.292200709026453	26.61576220343605	23.58535587673848	26.506681210799016
18	25.15339514589583	22.061630760839922	23.598991000818106	29.185983092446143
19	26.383965094082356	23.929642759749115	25.344286883010636	24.342105263157894
20	26.806653940550856	22.12980638123807	24.458003817834744	26.605535860376328
21	27.35887646577584	22.044586855740388	23.503545132260705	27.09299154622307
22	28.013362421598035	22.399100081810744	22.55590400872648	27.031633487864738
23	26.912326152167985	22.06844832287974	23.517180256340335	27.502045268611948
24	24.76138532860649	24.703436051268067	23.30583583310608	27.229342787019363
25	27.39296427597491	22.450231797109353	22.838832833378785	27.317971093536954
26	24.672757022088902	24.192118898281976	23.69443686937551	27.44068721025361
27	25.89310062721571	24.355740387237525	23.234251431688026	26.516907553858744
28	25.484046904826833	22.45364057812926	25.08862830651759	26.973684210526315
29	25.57949277338424	22.924052358876466	25.705617671120805	25.790837196618487
30	25.991955276793018	22.688846468502863	25.22838832833379	26.09080992637033
31	25.28633760567221	23.138805563130624	23.926233978729204	27.64862285246796
32	25.732887919280067	23.176302154349603	25.35110444505045	25.73970548131988
33	25.541996182165256	22.47750204526861	25.460185437687482	26.520316334878647
34	26.145350422688846	23.363785110444503	24.37278429233706	26.118080174529588
35	25.831742568857376	22.589991818925554	26.85096809380965	24.72729751840742
36	25.971502590673573	22.39228251977093	23.939869102808835	27.696345786746658
37	25.616989364603214	21.659394600490863	25.480638123806926	27.242977911098993
38	24.369375511317156	22.01731660758113	26.85778565584947	26.75552222525225
39	24.689800927188436	21.51622579765476	27.34524134169621	26.448731933460596
40	23.541041723479683	21.25034087810199	29.475729479138263	25.732887919280067
41	23.5955822197982	21.2060267248432	28.026997545677666	27.171393509680936
42	22.65475865830379	20.86173984183256	30.276793018816473	26.206708481047176
43	21.734387782928824	21.083310608126535	29.820016362148895	27.362285246795746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	6.0
4	9.0
5	9.0
6	10.0
7	11.0
8	13.0
9	15.0
10	16.5
11	18.0
12	18.0
13	40.0
14	62.0
15	78.5
16	95.0
17	86.0
18	77.0
19	77.0
20	92.5
21	108.0
22	80.5
23	53.0
24	62.5
25	72.0
26	72.0
27	87.0
28	102.0
29	154.5
30	207.0
31	293.0
32	379.0
33	379.0
34	540.5
35	702.0
36	788.5
37	875.0
38	1027.5
39	1180.0
40	1180.0
41	1407.5
42	1635.0
43	1845.5
44	2056.0
45	2239.5
46	2423.0
47	2423.0
48	2561.0
49	2699.0
50	2737.5
51	2776.0
52	2743.0
53	2710.0
54	2710.0
55	2402.5
56	2095.0
57	2100.5
58	2106.0
59	1817.0
60	1528.0
61	1528.0
62	1310.5
63	1093.0
64	915.0
65	737.0
66	686.0
67	635.0
68	635.0
69	570.0
70	505.0
71	431.0
72	357.0
73	402.5
74	448.0
75	448.0
76	413.5
77	379.0
78	376.5
79	374.0
80	341.5
81	309.0
82	309.0
83	278.0
84	247.0
85	193.0
86	139.0
87	116.0
88	93.0
89	93.0
90	55.0
91	17.0
92	11.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	29336.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.73834196891191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.77858439201452	53.75988546495773
2	9.515167228415867	12.510226343059722
3	3.2875291677469534	6.483501499863649
4	1.866735805029816	4.908644668666485
5	1.0733730878921441	3.528088355604036
6	0.6378014000518538	2.515680392691573
7	0.44594244231267827	2.052086173984183
8	0.3318641431164117	1.745295882192528
9	0.17630282603059372	1.043086992091628
>10	0.8452164894996111	8.583310608126533
>50	0.020741508944775732	0.9067357512953367
>100	0.020741508944775732	1.9634578674665941
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	261	0.8896918461958003	RNA PCR Primer, Index 26 (100% over 22bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	108	0.36814835014998637	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	106	0.36133078811017183	No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGG	101	0.34428688301063537	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	85	0.2897463866921189	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	66	0.22497954731388056	RNA PCR Primer, Index 26 (100% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	60	0.20452686119443686	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.18748295609490045	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.1636214889555495	No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCC	45	0.15339514589582764	No Hit
CCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC	42	0.1431688028361058	No Hit
TGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTG	34	0.11589855467684755	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.11248977365694028	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	32	0.109080992637033	No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACA	31	0.10567221161712573	No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGG	31	0.10567221161712573	No Hit
CCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACG	31	0.10567221161712573	No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT	30	0.10226343059721843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.010226343059721843	0.0
25	0.0	0.0	0.0	0.017043905099536405	0.0
26	0.0	0.0	0.0	0.02727024815925825	0.0
27	0.0	0.0	0.0	0.06476683937823834	0.0
28	0.0	0.0	0.0	0.14657758385601308	0.0
29	0.0	0.0	0.0	0.2897463866921189	0.0
30	0.0	0.0	0.0	0.44655031360785385	0.0
31	0.0	0.0	0.0	1.032860649031906	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTAAC	30	8.038758E-6	37.0	2
CTAACAC	30	8.038758E-6	37.0	4
GCCGTCT	35	2.3104185E-5	31.714287	36
AAGGTTG	25	0.005410449	29.6	5
TATACAC	40	5.747939E-5	27.75	3
CCTAACA	40	5.747939E-5	27.75	3
CCGTCTT	40	5.747939E-5	27.75	37
TTATACA	40	5.747939E-5	27.75	2
ATGCCGT	40	5.747939E-5	27.75	34
GGTATCA	65	8.756615E-8	25.615385	1
TGCCGTC	45	1.2807398E-4	24.666668	35
TATGCCG	45	1.2807398E-4	24.666668	33
GTCCTAA	45	1.2807398E-4	24.666668	1
CGTATGC	45	1.2807398E-4	24.666668	31
TCTTATA	40	0.0018868772	23.125	37
ACACGTG	50	2.6160627E-4	22.2	7
GTATGCC	50	2.6160627E-4	22.2	32
CACGTGC	50	2.6160627E-4	22.2	8
CGCTCGT	50	2.6160627E-4	22.2	14
ACACATC	50	2.6160627E-4	22.2	6
>>END_MODULE