FastQCFastQC Report
Fri 10 Feb 2017
ERR1632229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426967
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29140.20420934751819766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24710.17316448102864326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19100.13385032730259355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.019.9776361
CGCCTAT752.0677072E-417.2666661
GACCTAT650.001580186717.0769237
ACTAGCA1059.34618E-615.8571422
TCGGTGA2250.015.62222213
ATAATAC1101.4522773E-515.1363643
TCTTATA16150.015.12074337
GATATAT750.00410505214.81
TACACTT1152.2104738E-514.478265
TTATACT1551.210592E-714.3225814
GTATTAA1304.445483E-614.2307691
CTTATAC9750.014.04102537
ACTATTC1854.9003575E-914.03
CCGTATT800.006299603313.87500114
AATGGTA1203.301457E-513.8749992
TAGACTG2051.4242687E-913.5365865
CGGTGAT2601.8189894E-1213.51923114
TATACTG1102.4570109E-413.4545455
GTATCAA23400.013.4401712
TATACAC4150.013.37349437
Produced by FastQC (version 0.11.5)