FastQCFastQC Report
Fri 10 Feb 2017
ERR1632228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162292
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1960.12076997017721144RNA PCR Primer, Index 17 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACAG352.3757791E-531.7142855
GGTATCA1500.029.61
TATGACT250.00548261429.5999984
CTAGAAT400.001924086423.1254
AAGGCCC400.001924086423.12527
GTATCAA1950.022.7692322
GCTTTGA609.1902923E-418.51
AGAGTCT500.00700950818.49999815
ATCAACG2450.018.1224484
CAACGCA2600.017.0769236
TCAACGC2600.017.0769235
TATCAAC2700.016.4444433
TCTTATA2050.016.24390237
TAGAACA700.002579649415.8571424
GTAGAAC700.002579649415.8571423
TGTAGAA1059.244517E-615.8571422
AACGCAG3100.014.9193557
ATGGGAA1152.1867023E-514.47826119
CTGTAGA800.006267932713.8751
ATCTAAG800.006267932713.87526
Produced by FastQC (version 0.11.5)