##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162292
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78923791684125	34.0	31.0	34.0	31.0	34.0
2	32.95224656791462	34.0	33.0	34.0	31.0	34.0
3	32.94963399304957	34.0	33.0	34.0	31.0	34.0
4	36.38615581790846	37.0	37.0	37.0	35.0	37.0
5	36.311019643605356	37.0	37.0	37.0	35.0	37.0
6	36.34031868483967	37.0	37.0	37.0	35.0	37.0
7	36.339067853005695	37.0	37.0	37.0	35.0	37.0
8	36.319202425258176	37.0	37.0	37.0	35.0	37.0
9	38.11806496931457	39.0	39.0	39.0	37.0	39.0
10	38.05112390013063	39.0	38.0	39.0	35.0	39.0
11	38.14700046829172	39.0	39.0	39.0	37.0	39.0
12	38.12870012076997	39.0	38.0	39.0	37.0	39.0
13	38.145034875409756	39.0	39.0	39.0	37.0	39.0
14	39.5567372390506	41.0	39.0	41.0	37.0	41.0
15	39.58756439011165	41.0	39.0	41.0	37.0	41.0
16	39.471619057008354	41.0	39.0	41.0	37.0	41.0
17	39.51884258004091	41.0	39.0	41.0	37.0	41.0
18	39.52990289108521	41.0	39.0	41.0	37.0	41.0
19	39.58108840854756	41.0	39.0	41.0	37.0	41.0
20	39.574384442856086	41.0	39.0	41.0	37.0	41.0
21	39.547747270352204	41.0	39.0	41.0	37.0	41.0
22	39.5210484805166	41.0	39.0	41.0	37.0	41.0
23	39.482679368052644	41.0	39.0	41.0	37.0	41.0
24	39.4211914327262	41.0	39.0	41.0	37.0	41.0
25	39.37431912848446	41.0	39.0	41.0	37.0	41.0
26	39.28879427205284	40.0	39.0	41.0	36.0	41.0
27	39.228772829221406	40.0	39.0	41.0	36.0	41.0
28	39.151073373918614	40.0	39.0	41.0	36.0	41.0
29	39.026372217977475	40.0	39.0	41.0	36.0	41.0
30	38.99466393907278	40.0	39.0	41.0	35.0	41.0
31	38.939424001183056	40.0	39.0	41.0	35.0	41.0
32	38.873838513297024	40.0	38.0	41.0	35.0	41.0
33	38.73278411751657	40.0	38.0	41.0	35.0	41.0
34	38.73726369753284	40.0	38.0	41.0	35.0	41.0
35	38.69500036970399	40.0	38.0	41.0	35.0	41.0
36	38.680187563157766	40.0	38.0	41.0	35.0	41.0
37	38.646335001109115	40.0	38.0	41.0	35.0	41.0
38	38.58473615458556	40.0	38.0	41.0	35.0	41.0
39	38.40383383037981	40.0	38.0	41.0	34.0	41.0
40	38.410605575136174	40.0	38.0	41.0	34.0	41.0
41	38.408664629186895	40.0	38.0	41.0	34.0	41.0
42	38.27644615877554	40.0	38.0	41.0	34.0	41.0
43	37.44212284031252	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	0.0
19	10.0
20	6.0
21	21.0
22	35.0
23	63.0
24	105.0
25	157.0
26	236.0
27	325.0
28	442.0
29	614.0
30	921.0
31	1218.0
32	1749.0
33	2486.0
34	3740.0
35	5660.0
36	9050.0
37	16567.0
38	39640.0
39	79245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68047716461687	17.756266482636235	12.605673723905058	26.95758262884184
2	20.603603381559164	20.004066743893723	32.41872673945728	26.973603135089835
3	21.29125280358859	20.890740147388655	28.488157148842824	29.329849900179923
4	16.16099376432603	15.574396766322431	31.646661572967243	36.617947896384294
5	17.041505434648656	33.78416681044044	32.392231286816354	16.782096468094547
6	36.73502082665812	32.00835531018165	14.754270081088409	16.502353782071822
7	30.519064402435113	28.329800606314542	20.008379957114336	21.142755034136
8	27.997683188327212	31.32132206146945	19.33921573460183	21.34177901560151
9	26.900278510339387	13.67103738939689	18.77110393611515	40.65758016414857
10	18.694082271461316	25.247085500209497	31.365686540310055	24.693145688019126
11	37.547137258768146	20.49515687772657	19.636827446824242	22.320878416681044
12	22.866191802430187	24.31111823133611	26.83003475217509	25.992655214058612
13	31.822887141695215	18.138910112636484	23.120671382446453	26.917531363221848
14	24.7609247529145	20.004066743893723	22.422547014024104	32.812461489167674
15	27.653858477312497	25.935967268873387	20.172282059497697	26.23789219431642
16	27.542947280210978	24.1823380080349	21.910506987405416	26.364207724348702
17	25.5946072511276	25.086880437729526	23.20940034012767	26.10911197101521
18	25.369703990338405	23.256845685554435	24.719024967342815	26.65442535676435
19	26.693860449067113	24.09052818376753	23.775663618662655	25.4399477485027
20	27.006260320903063	23.017770438468933	23.947575974170014	26.02839326645799
21	27.390752470855002	23.580952850417766	22.7940995243142	26.23419515441303
22	27.13011115766643	23.25376481896828	22.7478865255219	26.868237497843396
23	26.184901289034578	23.443546200675325	23.298129297808888	27.073423212481206
24	26.629162250757894	23.8508367633648	23.141621275232296	26.37837971064501
25	26.847287605057552	23.341261430015035	23.04180119784093	26.769649767086484
26	26.688314889212037	24.08128558400907	23.104650876198455	26.125748650580434
27	27.008108840854756	23.099721489660613	23.119439035811993	26.772730633672637
28	25.924876149163232	23.454021147068246	24.098538436891527	26.522564266876987
29	25.494171000418998	23.617307076134374	24.229167180144433	26.659354743302195
30	25.550242772286992	24.406008922189635	24.291400685184726	25.75234762033865
31	26.746851354348948	23.684469967712516	23.280260271609198	26.288418406329335
32	25.518201759790994	23.495920932639933	23.855149976585412	27.13072733098366
33	25.570576491755602	23.3264732704015	23.94511128090109	27.15783895694181
34	26.244670100805955	23.210632686762132	23.84405885687526	26.70063835555665
35	25.95568481502477	22.999285238952012	24.78988489882434	26.255145047198873
36	25.319793951642716	23.3344835235255	24.350553323638874	26.99516920119291
37	26.022847706602914	21.74722105833929	25.325339511497795	26.90459172356
38	24.9377664949597	22.121238261898306	26.153476449855816	26.787518793286175
39	24.98089862716585	21.595642422300546	26.86330811130555	26.560150839228058
40	23.97037438690755	21.447760826165183	27.698839129470336	26.883025657456926
41	23.375767135779952	21.800211963621127	28.404973751016687	26.419047149582237
42	23.947575974170014	21.440982919675648	28.016168387844132	26.59527271831021
43	22.469376186133637	21.25058536465137	28.814112833657852	27.465925615557147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	4.5
2	6.0
3	8.5
4	11.0
5	11.0
6	17.0
7	23.0
8	25.0
9	27.0
10	36.5
11	46.0
12	46.0
13	75.0
14	104.0
15	164.0
16	224.0
17	233.5
18	243.0
19	243.0
20	296.0
21	349.0
22	427.0
23	505.0
24	660.0
25	815.0
26	815.0
27	935.0
28	1055.0
29	1482.5
30	1910.0
31	2342.5
32	2775.0
33	2775.0
34	3212.0
35	3649.0
36	4332.5
37	5016.0
38	5812.5
39	6609.0
40	6609.0
41	7359.5
42	8110.0
43	8443.5
44	8777.0
45	9611.0
46	10445.0
47	10445.0
48	11219.5
49	11994.0
50	12735.5
51	13477.0
52	14312.5
53	15148.0
54	15148.0
55	14380.0
56	13612.0
57	13034.0
58	12456.0
59	11574.5
60	10693.0
61	10693.0
62	9995.5
63	9298.0
64	8396.5
65	7495.0
66	6654.5
67	5814.0
68	5814.0
69	4969.0
70	4124.0
71	3574.0
72	3024.0
73	2432.0
74	1840.0
75	1840.0
76	1476.5
77	1113.0
78	880.0
79	647.0
80	539.5
81	432.0
82	432.0
83	323.5
84	215.0
85	166.5
86	118.0
87	85.5
88	53.0
89	53.0
90	40.5
91	28.0
92	17.0
93	6.0
94	4.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	162292.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.94926697966889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.83440572603043	61.250671573842986
2	5.868154057405782	8.09209841563797
3	1.7554784976013678	3.631168668245542
4	0.8152430792057047	2.2484165088592594
5	0.5451892579029128	1.879519984879775
6	0.3304789129853572	1.3671767281546896
7	0.28255041717502205	1.3637150904312194
8	0.20667067634227093	1.1399833311993581
9	0.14776448681136517	0.9169427746143521
>10	1.149168327981988	14.891369904272322
>50	0.05790770019004861	2.6220981840582622
>100	0.006988860367764487	0.5968388358042829
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	196	0.12076997017721144	RNA PCR Primer, Index 17 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.161733172306707E-4	0.0	0.0	0.0
6	0.0	6.161733172306707E-4	0.0	0.0	0.0
7	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
8	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
9	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
10	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
11	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
12	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
13	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
14	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
15	0.0	6.161733172306707E-4	0.0	6.161733172306707E-4	0.0
16	0.0	6.161733172306707E-4	0.0	0.0012323466344613414	0.0
17	0.0	6.161733172306707E-4	0.0	0.001848519951692012	0.0
18	0.0	6.161733172306707E-4	0.0	0.0024646932689226827	0.0
19	0.0	6.161733172306707E-4	0.0	0.0024646932689226827	0.0
20	0.0	6.161733172306707E-4	0.0	0.003080866586153353	0.0
21	0.0	6.161733172306707E-4	0.0	0.003697039903384024	0.0
22	0.0	6.161733172306707E-4	0.0	0.005545559855076035	0.0
23	0.0	6.161733172306707E-4	0.0	0.00862642644122939	0.0
24	0.0	6.161733172306707E-4	0.0	0.01109111971015207	0.0
25	0.0	6.161733172306707E-4	0.0	0.012939639661844084	0.0
26	0.0	6.161733172306707E-4	0.0	0.02156606610307347	0.0
27	0.0	6.161733172306707E-4	0.0	0.04744534542676164	0.0
28	0.0	6.161733172306707E-4	0.0	0.11399206368767408	0.0
29	0.0	6.161733172306707E-4	0.0	0.20888275454119734	0.0
30	0.0	6.161733172306707E-4	0.0	0.33334976462179283	0.0
31	0.0	6.161733172306707E-4	0.0	0.6488305030438962	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACAG	35	2.3757791E-5	31.714285	5
GGTATCA	150	0.0	29.6	1
TATGACT	25	0.005482614	29.599998	4
CTAGAAT	40	0.0019240864	23.125	4
AAGGCCC	40	0.0019240864	23.125	27
GTATCAA	195	0.0	22.769232	2
GCTTTGA	60	9.1902923E-4	18.5	1
AGAGTCT	50	0.007009508	18.499998	15
ATCAACG	245	0.0	18.122448	4
CAACGCA	260	0.0	17.076923	6
TCAACGC	260	0.0	17.076923	5
TATCAAC	270	0.0	16.444443	3
TCTTATA	205	0.0	16.243902	37
TAGAACA	70	0.0025796494	15.857142	4
GTAGAAC	70	0.0025796494	15.857142	3
TGTAGAA	105	9.244517E-6	15.857142	2
AACGCAG	310	0.0	14.919355	7
ATGGGAA	115	2.1867023E-5	14.478261	19
CTGTAGA	80	0.0062679327	13.875	1
ATCTAAG	80	0.0062679327	13.875	26
>>END_MODULE