FastQCFastQC Report
Fri 10 Feb 2017
ERR1632227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006037
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72390.3608607418507236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51650.2574728182979676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45370.22616731396280326No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC38700.19291767798899023No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA38240.19062459964596865No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG37570.1872846811898285No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG27280.13598951564701947No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT26910.13414508306676298No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC23490.11709654408168942No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA22880.11405572280072601No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.11206174163288114No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG21930.10932001752709446No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT21850.1089212212935255No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA21640.10787438118040693No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA21620.10777468212201471No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC21520.10727618683005348No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC20090.10014770415500811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.019.63411
TCTACAC1355.638867E-1119.1851863
CTATAAG953.6091005E-617.5263161
CTTATAC12500.017.46437
CGGCCTT10700.017.28971924
TACACCG752.0682346E-417.2666665
TCGCCAT11050.016.90950213
TAATACT1655.4569682E-1116.8181824
GTAATAC1107.814742E-716.8181823
TCGTTTA1452.9831426E-916.58620630
TAGCACT4250.016.5411784
TAGGACA10250.016.4243894
ATAATAT2050.016.2439043
ACGTTAA803.3841378E-416.187520
TAATTCG803.3841378E-416.18755
GCTTAGG9800.015.8571421
CCATTGT12250.015.85714216
CTAGCAC4450.015.7977523
TCTCGCC12000.015.72512
TCGGCCT12050.015.50622423
Produced by FastQC (version 0.11.5)