##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632227.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2006037
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79251579108461	34.0	31.0	34.0	31.0	34.0
2	32.94759717791845	34.0	33.0	34.0	31.0	34.0
3	32.94018604841287	34.0	33.0	34.0	31.0	34.0
4	36.39129637190142	37.0	37.0	37.0	35.0	37.0
5	36.31727380900751	37.0	37.0	37.0	35.0	37.0
6	36.35259269893825	37.0	37.0	37.0	35.0	37.0
7	36.34106449681636	37.0	37.0	37.0	35.0	37.0
8	36.32870480454747	37.0	37.0	37.0	35.0	37.0
9	38.10106742796868	39.0	39.0	39.0	37.0	39.0
10	38.04867656977414	39.0	38.0	39.0	37.0	39.0
11	38.14406613636738	39.0	39.0	39.0	37.0	39.0
12	38.12629926566659	39.0	38.0	39.0	37.0	39.0
13	38.140522831832115	39.0	39.0	39.0	37.0	39.0
14	39.55580430470624	41.0	39.0	41.0	37.0	41.0
15	39.59101601814922	41.0	39.0	41.0	37.0	41.0
16	39.46773514147546	41.0	39.0	41.0	37.0	41.0
17	39.53412923091648	41.0	39.0	41.0	37.0	41.0
18	39.55069273398247	41.0	39.0	41.0	37.0	41.0
19	39.61510081818032	41.0	39.0	41.0	37.0	41.0
20	39.600015353654996	41.0	39.0	41.0	37.0	41.0
21	39.57098498183234	41.0	39.0	41.0	37.0	41.0
22	39.55759539829026	41.0	39.0	41.0	37.0	41.0
23	39.517399728918264	41.0	39.0	41.0	37.0	41.0
24	39.47218919690913	41.0	39.0	41.0	37.0	41.0
25	39.41401030987963	41.0	39.0	41.0	37.0	41.0
26	39.3329629513314	40.0	39.0	41.0	36.0	41.0
27	39.262673619679	40.0	39.0	41.0	36.0	41.0
28	39.19690514182939	40.0	39.0	41.0	36.0	41.0
29	39.060569171954455	40.0	39.0	41.0	36.0	41.0
30	39.01506751869482	40.0	39.0	41.0	35.0	41.0
31	38.93493739148381	40.0	38.0	41.0	35.0	41.0
32	38.86331657890657	40.0	38.0	41.0	35.0	41.0
33	38.72662219091672	40.0	38.0	41.0	35.0	41.0
34	38.72515113131014	40.0	38.0	41.0	35.0	41.0
35	38.695653170903626	40.0	38.0	41.0	35.0	41.0
36	38.6567117156862	40.0	38.0	41.0	35.0	41.0
37	38.61834701952157	40.0	38.0	41.0	35.0	41.0
38	38.53754192968525	40.0	38.0	41.0	35.0	41.0
39	38.35095364641828	40.0	38.0	41.0	34.0	41.0
40	38.35015405996998	40.0	38.0	41.0	34.0	41.0
41	38.329601597577714	40.0	38.0	41.0	34.0	41.0
42	38.18762415648366	40.0	38.0	41.0	34.0	41.0
43	37.37268754265251	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	5.0
13	2.0
14	2.0
15	5.0
16	7.0
17	20.0
18	26.0
19	49.0
20	124.0
21	227.0
22	431.0
23	720.0
24	1138.0
25	1847.0
26	2731.0
27	4018.0
28	5956.0
29	8543.0
30	12028.0
31	16590.0
32	22037.0
33	30778.0
34	45076.0
35	67654.0
36	109880.0
37	208929.0
38	474675.0
39	992537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08529703091219	18.077582816269093	12.805945254250044	26.031174898568672
2	18.28670159124682	21.140387739607995	34.71252025760243	25.86039041154276
3	19.614693049031498	22.367583449358115	31.233621314063498	26.78410218754689
4	14.886016559016607	15.760576699233365	34.04209393944379	35.31131280230624
5	14.868569223797968	36.26124543066753	33.80171950966009	15.068465835874415
6	34.0844161897313	34.70289929846758	16.06650325990996	15.146181251891166
7	28.763876239570855	30.920865367887036	20.904400068393553	19.41085832414856
8	27.296355949566237	33.543947594186946	20.17131289203539	18.98838356421143
9	26.46172528223557	14.671563884414892	19.179456809620163	39.68725402372937
10	16.98797180710027	27.304481422825198	32.409172911566436	23.298373858508093
11	35.71987954359765	21.63798574004368	21.582702612165182	21.059432104193494
12	21.91121100956762	25.607703148047616	28.331082627090126	24.150003215294632
13	30.48762310964354	19.152637762912647	25.00656767547159	25.353171451972223
14	23.016724018550004	20.504307747065482	24.616345560924348	31.862622673460162
15	25.742994770285893	27.425366531125796	22.21554238530994	24.61609631327837
16	25.42515417213142	25.992790761087658	23.449467781501536	25.132587285279385
17	24.361764015319757	26.429771734020857	24.606076557909947	24.602387692749435
18	23.33581085493438	24.945850948910714	26.517806002581207	25.2005321935737
19	25.062897643463206	25.319822116940017	26.44951214758252	23.167768092014253
20	25.250282023711428	24.31460636070023	26.587096848163817	23.84801476742453
21	25.103275762112066	25.487964578918536	24.905871626495426	24.502888032473976
22	25.257360656857276	24.735436086173884	25.02650748715004	24.9806957698188
23	24.14127954768531	25.474604905093972	25.486867889276223	24.897247657944494
24	25.511344008111514	25.140114564188	25.247490450076445	24.10105097762404
25	25.061651405233302	24.383797507224443	25.727092770472332	24.827458317069926
26	24.98538162556324	24.640422883526075	25.473109419218094	24.901086071692596
27	24.74974290105317	24.58793132928256	25.367976762143467	25.2943490075208
28	23.866708340873075	25.265934775879007	26.101063938501635	24.766292944746283
29	24.274377790638958	25.728987052581783	25.689605924516844	24.307029232262416
30	23.64572537794667	26.504446328756647	25.55765422073471	24.292174072561973
31	24.737878713104493	25.353520398676594	25.826592430747787	24.082008457471122
32	23.633811340468792	25.50795424012618	25.87823654299497	24.979997876410057
33	23.88221154445307	25.005770083004453	26.173794401598776	24.938223970943707
34	24.16884633733077	25.022968170577116	25.93361936993186	24.87456612216026
35	24.333299934148773	25.090265034991877	25.915922787067235	24.660512243792112
36	23.706990449328703	25.35790715724585	26.161581266945728	24.77352112647972
37	24.775315709530783	23.683162374372955	26.14682580630367	25.39469610979259
38	23.595377353458584	24.40064664809273	27.363353716805822	24.64062228164286
39	23.691537095277905	23.905291876470873	27.617935262410416	24.78523576584081
40	23.256799351158527	23.398172615958728	28.457700431248277	24.887327601634464
41	22.273068742002266	24.052946181949785	28.986105440727165	24.687879635320783
42	23.067022193508894	23.06652369821693	28.58247380282617	25.283980305448004
43	21.7687909046543	23.317964723482167	29.185453707982457	25.727790663881073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	93.5
2	110.0
3	420.5
4	731.0
5	731.0
6	998.0
7	1265.0
8	1340.5
9	1416.0
10	2179.5
11	2943.0
12	2943.0
13	4880.5
14	6818.0
15	11372.0
16	15926.0
17	14726.0
18	13526.0
19	13526.0
20	14783.0
21	16040.0
22	16407.0
23	16774.0
24	18850.5
25	20927.0
26	20927.0
27	23560.0
28	26193.0
29	35621.0
30	45049.0
31	48175.0
32	51301.0
33	51301.0
34	57598.5
35	63896.0
36	69134.0
37	74372.0
38	88412.0
39	102452.0
40	102452.0
41	113899.5
42	125347.0
43	120822.5
44	116298.0
45	126032.0
46	135766.0
47	135766.0
48	143743.0
49	151720.0
50	158669.5
51	165619.0
52	174522.5
53	183426.0
54	183426.0
55	158911.5
56	134397.0
57	125650.5
58	116904.0
59	106845.0
60	96786.0
61	96786.0
62	90283.5
63	83781.0
64	75037.0
65	66293.0
66	59040.0
67	51787.0
68	51787.0
69	45328.5
70	38870.0
71	34287.0
72	29704.0
73	24365.5
74	19027.0
75	19027.0
76	15319.0
77	11611.0
78	9951.0
79	8291.0
80	6531.0
81	4771.0
82	4771.0
83	3711.5
84	2652.0
85	2149.5
86	1647.0
87	1287.0
88	927.0
89	927.0
90	698.5
91	470.0
92	280.0
93	90.0
94	58.0
95	26.0
96	26.0
97	17.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2006037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.3414878333359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.38459845737005	42.983877243959654
2	8.326624023369318	8.383492839304179
3	2.3483226843237155	3.54654173524887
4	1.0715512853581504	2.157739439786111
5	0.6112730028308305	1.5386196217427477
6	0.3985777815508971	1.2038999124349499
7	0.2719332136251007	0.9582665795631556
8	0.19699054235209051	0.7933437592880004
9	0.14585257301142332	0.6608191976743772
>10	0.9858169142215085	9.59900622200902
>50	0.12051741954083918	4.252866269889071
>100	0.1145207602662139	11.972328413875168
>500	0.016743768026482826	5.679582214576086
>1k	0.0064782435816749025	5.647270810457826
>5k	1.993305717438432E-4	0.6223457401908283
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7239	0.3608607418507236	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5165	0.2574728182979676	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4537	0.22616731396280326	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3870	0.19291767798899023	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3824	0.19062459964596865	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3757	0.1872846811898285	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2728	0.13598951564701947	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2691	0.13414508306676298	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2349	0.11709654408168942	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2288	0.11405572280072601	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2248	0.11206174163288114	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	2193	0.10932001752709446	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2185	0.1089212212935255	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2164	0.10787438118040693	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	2162	0.10777468212201471	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2152	0.10727618683005348	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2009	0.10014770415500811	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4954858758836452E-4	0.0
11	0.0	0.0	0.0	1.4954858758836452E-4	0.0
12	0.0	0.0	0.0	1.9939811678448602E-4	0.0
13	0.0	0.0	0.0	1.9939811678448602E-4	0.0
14	0.0	0.0	0.0	3.4894670437285056E-4	0.0
15	0.0	0.0	0.0	4.4864576276509356E-4	0.0
16	0.0	0.0	0.0	0.0014456363466875237	0.0
17	0.0	0.0	0.0	0.002741724105786683	0.0
18	0.0	0.0	0.0	0.0031903698685517763	0.0
19	0.0	0.0	0.0	0.003738714689709113	0.0
20	0.0	0.0	0.0	0.004237209981670328	0.0
21	0.0	0.0	0.0	0.0053837491531811225	0.0
22	0.0	0.0	0.0	0.006879235029064768	0.0
23	0.0	0.0	0.0	0.0092720124304786	0.0
24	0.0	0.0	0.0	0.013758470058129536	0.0
25	0.0	0.0	0.0	0.016101397930347247	0.0
26	0.0	0.0	0.0	0.019341617328095143	0.0
27	0.0	0.0	0.0	0.03509406855406954	4.9849529196121504E-5
28	0.0	0.0	0.0	0.0845946510458182	4.9849529196121504E-5
29	0.0	4.9849529196121504E-5	0.0	0.1694883992668131	4.9849529196121504E-5
30	0.0	4.9849529196121504E-5	0.0	0.2918191439140953	4.9849529196121504E-5
31	0.0	4.9849529196121504E-5	0.0	0.5812953599559729	4.9849529196121504E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2610	0.0	19.6341	1
TCTACAC	135	5.638867E-11	19.185186	3
CTATAAG	95	3.6091005E-6	17.526316	1
CTTATAC	1250	0.0	17.464	37
CGGCCTT	1070	0.0	17.289719	24
TACACCG	75	2.0682346E-4	17.266666	5
TCGCCAT	1105	0.0	16.909502	13
TAATACT	165	5.4569682E-11	16.818182	4
GTAATAC	110	7.814742E-7	16.818182	3
TCGTTTA	145	2.9831426E-9	16.586206	30
TAGCACT	425	0.0	16.541178	4
TAGGACA	1025	0.0	16.424389	4
ATAATAT	205	0.0	16.243904	3
ACGTTAA	80	3.3841378E-4	16.1875	20
TAATTCG	80	3.3841378E-4	16.1875	5
GCTTAGG	980	0.0	15.857142	1
CCATTGT	1225	0.0	15.857142	16
CTAGCAC	445	0.0	15.797752	3
TCTCGCC	1200	0.0	15.725	12
TCGGCCT	1205	0.0	15.506224	23
>>END_MODULE