FastQCFastQC Report
Fri 10 Feb 2017
ERR1632226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572103
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.2920928208902343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37400.2378978985473598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33660.21410810869262384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG303.6000763E-430.8333325
GGTATCA11450.024.558951
CTTATAC9250.018.637
TAAGACG701.21955934E-418.54
ATAACGA1401.869921E-917.17857212
TACCGTC1201.04189894E-716.9583327
CGAACGA1452.9813236E-916.58620616
ATACCGT1251.6587364E-716.2799996
CAATACG1302.590532E-715.6538464
GTATCAA18300.015.1639342
GTATTAT1353.9745464E-715.0740751
TAACGAA1601.0972144E-815.03125113
TACGCTA750.004105298314.89
ACGAACG1752.239176E-914.79999915
TCCGATA1651.6460035E-814.5757578
CCGATAA1651.6460035E-814.5757579
ACCGTCG1152.2107564E-514.478268
AATTCCG1803.3323886E-914.3888895
TAGGACA4250.014.3647064
TAATACC1702.4381734E-814.1470594
Produced by FastQC (version 0.11.5)