FastQCFastQC Report
Fri 10 Feb 2017
ERR1632225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497898
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCA358.8623044E-426.4285724
GGTATCA3100.025.661291
CTCTATG601.3348636E-624.6666681
GTATTGA450.003822808620.5555551
GCAATAC450.003822808620.5555553
ACCTTAG450.003822808620.5555551
TGATAGA609.226793E-418.52
TAGGCTC500.007029419318.4999985
TATGCCG752.0643024E-417.26666633
TATACCA752.0643024E-417.2666665
GTATCAA4550.017.0769232
GGACCGC904.4378183E-516.4444456
AGTACAG1151.2386608E-616.0869565
GCGGGTA700.002589726615.85714323
CTTATAC7200.015.67361137
TCTTATA11350.014.99559537
TTCTAAG750.004100062414.82
CTATGCC750.004100062414.83
TAATAGA750.004100062414.84
TATGGGC750.004100062414.84
Produced by FastQC (version 0.11.5)