##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632225.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497898
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85247179141109	34.0	31.0	34.0	31.0	34.0
2	33.02778681577351	34.0	33.0	34.0	31.0	34.0
3	33.034661717861894	34.0	33.0	34.0	31.0	34.0
4	36.45386806132983	37.0	37.0	37.0	35.0	37.0
5	36.367693784670756	37.0	37.0	37.0	35.0	37.0
6	36.39274309195859	37.0	37.0	37.0	35.0	37.0
7	36.380977629956334	37.0	37.0	37.0	35.0	37.0
8	36.370158144840914	37.0	37.0	37.0	35.0	37.0
9	38.177291332762934	39.0	39.0	39.0	37.0	39.0
10	38.12256325592792	39.0	38.0	39.0	37.0	39.0
11	38.218426264013914	39.0	39.0	39.0	37.0	39.0
12	38.19693391015831	39.0	39.0	39.0	37.0	39.0
13	38.216393719195494	39.0	39.0	39.0	37.0	39.0
14	39.654290637841484	41.0	39.0	41.0	38.0	41.0
15	39.67615254530044	41.0	39.0	41.0	38.0	41.0
16	39.568104310521434	41.0	39.0	41.0	37.0	41.0
17	39.61149070693194	41.0	39.0	41.0	37.0	41.0
18	39.62468618070368	41.0	39.0	41.0	37.0	41.0
19	39.67403966274217	41.0	40.0	41.0	37.0	41.0
20	39.65380057762835	41.0	39.0	41.0	37.0	41.0
21	39.63505979136289	41.0	39.0	41.0	37.0	41.0
22	39.612991014223795	41.0	39.0	41.0	37.0	41.0
23	39.57862052066889	41.0	39.0	41.0	37.0	41.0
24	39.531466284259025	41.0	39.0	41.0	37.0	41.0
25	39.471502195228744	41.0	39.0	41.0	37.0	41.0
26	39.39561315771503	41.0	39.0	41.0	37.0	41.0
27	39.341692475165594	40.0	39.0	41.0	36.0	41.0
28	39.29170030809523	40.0	39.0	41.0	36.0	41.0
29	39.149928298567175	40.0	39.0	41.0	36.0	41.0
30	39.10861461584501	40.0	39.0	41.0	36.0	41.0
31	39.05413558600356	40.0	39.0	41.0	35.0	41.0
32	39.001994384391985	40.0	39.0	41.0	35.0	41.0
33	38.878937051363934	40.0	38.0	41.0	35.0	41.0
34	38.87624372863518	40.0	38.0	41.0	35.0	41.0
35	38.86017417222001	40.0	38.0	41.0	35.0	41.0
36	38.84008973725542	40.0	38.0	41.0	35.0	41.0
37	38.808772077815135	40.0	38.0	41.0	35.0	41.0
38	38.769326247544676	40.0	38.0	41.0	35.0	41.0
39	38.59403331606072	40.0	38.0	41.0	35.0	41.0
40	38.60121551000406	40.0	38.0	41.0	35.0	41.0
41	38.61273393345625	40.0	38.0	41.0	35.0	41.0
42	38.49683670149307	40.0	38.0	41.0	35.0	41.0
43	37.68378663903049	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	5.0
19	5.0
20	18.0
21	35.0
22	74.0
23	135.0
24	247.0
25	362.0
26	538.0
27	803.0
28	1126.0
29	1555.0
30	2209.0
31	3276.0
32	4397.0
33	6409.0
34	9947.0
35	16314.0
36	26696.0
37	50034.0
38	121650.0
39	252058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.032508666433685	17.281250376583156	12.0695403476214	27.616700609361754
2	19.840007391072067	19.122792218486516	33.23813311160117	27.799067278840244
3	20.792210452743333	20.618680934649266	28.4965997051605	30.09250890744691
4	15.875540773411423	14.865293694692488	32.47311698379989	36.7860485480962
5	16.985808338254017	33.71975786205207	32.64082201575423	16.65361178393968
6	37.09615222394949	32.44941735054168	14.74579130665317	15.708639118855668
7	31.376908523432512	28.118409794777243	19.85426733989693	20.65041434189332
8	28.901301069697006	31.46688679207388	18.995456900811007	20.636355237418105
9	27.68257755604562	13.437892901758994	18.125198333795275	40.75433120840011
10	18.42023868342512	25.16720292108022	31.548630442379768	24.863927953114896
11	37.80593615559813	20.05591506694142	19.942237165041835	22.19591161241861
12	23.711081386147363	23.662677897882702	27.228267637146566	25.397973078823373
13	32.623348557335035	17.99223937432968	22.322443552695532	27.06196851563975
14	24.45239788069042	19.100699340025468	23.08986981269256	33.35703296659155
15	27.29414458383042	25.190500865639148	20.425267825940253	27.09008672459018
16	26.98886117236864	23.922771330674152	22.0292108022125	27.059156694744708
17	25.374675134264447	24.723537752712403	23.600014460793176	26.301772652229978
18	25.83742051584863	23.27062972737388	24.201342443633035	26.69060731314446
19	26.58496318523071	23.50401086166243	24.107347287998746	25.803678665108116
20	26.624730366460604	23.47408505356519	23.736990307251688	26.164194272722526
21	27.551627040076482	23.16558813250907	22.962735339366695	26.320049488047754
22	27.656668634941294	23.510638725200746	22.85347601315932	25.979216626698644
23	27.05795162864683	23.3087901538066	22.760083390573975	26.87317482697259
24	26.892255040188957	23.2991496250236	23.04950009841373	26.759095236373714
25	26.62533289950954	22.924976601633265	23.33931849495278	27.110372003904416
26	26.49759589313474	23.182459057879328	23.595595885100966	26.724349163884973
27	26.440154409136007	23.51907418788587	23.277860123961133	26.762911279016986
28	26.277269641573177	23.04950009841373	23.91453671233867	26.758693547674422
29	26.177048311099867	23.41744694696504	24.264608413771498	26.1408963281636
30	26.027820959312955	23.583344379772562	23.64379852901598	26.7450361318985
31	26.228464464609218	23.473683364865895	23.399973488545847	26.897878681979037
32	25.68056107877517	23.36201390646277	24.160771885004557	26.7966531297575
33	25.723541769599397	23.307183399009435	24.14209336048749	26.827181470903678
34	26.1999445669595	22.779966981188917	24.4142374542577	26.605850997593883
35	25.725148524396563	22.672515254128356	25.187287356044813	26.41504886543027
36	25.589578588385574	22.387115433281515	24.666096268713673	27.35720970961924
37	24.853082358234015	21.44856978738617	25.962747390027673	27.735600464352135
38	24.623918955288033	21.668494350248444	26.704465573270024	27.003121121193495
39	24.124419057718647	21.302355100843947	27.69764088226906	26.875584959168343
40	23.739400439447436	20.79883831628165	28.94769611446521	26.514065129805704
41	23.02339033295976	20.843224917553393	29.04349886924631	27.089885880240534
42	22.329071416233848	21.01575021389923	29.424701444874252	27.23047692499267
43	21.676728968583927	20.34734021827764	30.00473992665164	27.97119088648679
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	8.5
2	11.0
3	23.0
4	35.0
5	35.0
6	59.5
7	84.0
8	79.0
9	74.0
10	112.5
11	151.0
12	151.0
13	294.0
14	437.0
15	696.5
16	956.0
17	954.5
18	953.0
19	953.0
20	1243.5
21	1534.0
22	1690.0
23	1846.0
24	2402.5
25	2959.0
26	2959.0
27	3669.5
28	4380.0
29	5433.5
30	6487.0
31	7820.5
32	9154.0
33	9154.0
34	10894.5
35	12635.0
36	14860.0
37	17085.0
38	18584.0
39	20083.0
40	20083.0
41	22164.5
42	24246.0
43	26024.5
44	27803.0
45	29415.5
46	31028.0
47	31028.0
48	32359.5
49	33691.0
50	34997.0
51	36303.0
52	37727.5
53	39152.0
54	39152.0
55	39384.0
56	39616.0
57	38772.5
58	37929.0
59	35998.0
60	34067.0
61	34067.0
62	32382.0
63	30697.0
64	26897.0
65	23097.0
66	20450.5
67	17804.0
68	17804.0
69	15390.5
70	12977.0
71	11557.0
72	10137.0
73	9276.5
74	8416.0
75	8416.0
76	7311.5
77	6207.0
78	4552.0
79	2897.0
80	2208.5
81	1520.0
82	1520.0
83	1168.0
84	816.0
85	589.0
86	362.0
87	268.5
88	175.0
89	175.0
90	123.0
91	71.0
92	41.0
93	11.0
94	7.5
95	4.0
96	4.0
97	2.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	497898.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.03615607895789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.90183379158904	71.84531523415521
2	5.887261846177987	9.306130923044345
3	1.2592810044118448	2.9858619003588425
4	0.5763297045940015	1.8220353794092476
5	0.3100333642329016	1.2251922682598007
6	0.21144292473928572	1.002698160089132
7	0.14128962138863618	0.781689199788638
8	0.11377908966806094	0.7194129511621287
9	0.08285136734065554	0.5893428240442014
>10	0.4799747789007325	6.843275269411536
>50	0.025222187861892648	1.3428702866495508
>100	0.010700319094936106	1.5361756036273742
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.0168869929182283E-4	2.0084434964591142E-4
2	0.0	0.0	0.0	4.0168869929182283E-4	4.0168869929182283E-4
3	0.0	0.0	0.0	4.0168869929182283E-4	4.0168869929182283E-4
4	0.0	0.0	0.0	4.0168869929182283E-4	4.0168869929182283E-4
5	0.0	0.0	0.0	4.0168869929182283E-4	4.0168869929182283E-4
6	2.0084434964591142E-4	0.0	0.0	4.0168869929182283E-4	4.0168869929182283E-4
7	4.0168869929182283E-4	0.0	0.0	4.0168869929182283E-4	4.0168869929182283E-4
8	4.0168869929182283E-4	0.0	0.0	4.0168869929182283E-4	4.0168869929182283E-4
9	4.0168869929182283E-4	0.0	0.0	6.025330489377342E-4	4.0168869929182283E-4
10	4.0168869929182283E-4	0.0	0.0	8.033773985836457E-4	4.0168869929182283E-4
11	4.0168869929182283E-4	0.0	0.0	8.033773985836457E-4	4.0168869929182283E-4
12	4.0168869929182283E-4	0.0	0.0	8.033773985836457E-4	4.0168869929182283E-4
13	4.0168869929182283E-4	0.0	0.0	8.033773985836457E-4	4.0168869929182283E-4
14	4.0168869929182283E-4	0.0	0.0	0.0010042217482295571	4.0168869929182283E-4
15	6.025330489377342E-4	0.0	0.0	0.0010042217482295571	4.0168869929182283E-4
16	6.025330489377342E-4	0.0	0.0	0.0016067547971672913	4.0168869929182283E-4
17	6.025330489377342E-4	0.0	0.0	0.0020084434964591142	4.0168869929182283E-4
18	6.025330489377342E-4	0.0	0.0	0.0020084434964591142	4.0168869929182283E-4
19	8.033773985836457E-4	0.0	0.0	0.0022092878461050253	4.0168869929182283E-4
20	8.033773985836457E-4	0.0	0.0	0.0022092878461050253	4.0168869929182283E-4
21	0.0010042217482295571	0.0	0.0	0.002410132195750937	6.025330489377342E-4
22	0.0010042217482295571	0.0	0.0	0.003414353943980494	6.025330489377342E-4
23	0.0010042217482295571	0.0	0.0	0.005021108741147785	6.025330489377342E-4
24	0.0010042217482295571	0.0	0.0	0.007632085286544634	6.025330489377342E-4
25	0.0010042217482295571	0.0	0.0	0.009640528783003748	6.025330489377342E-4
26	0.0010042217482295571	0.0	0.0	0.016268392321318825	6.025330489377342E-4
27	0.0010042217482295571	0.0	0.0	0.0492068656632483	6.025330489377342E-4
28	0.0010042217482295571	0.0	0.0	0.1442062430457644	6.025330489377342E-4
29	0.0010042217482295571	0.0	0.0	0.27957533470710866	6.025330489377342E-4
30	0.0010042217482295571	0.0	0.0	0.4681681790246195	6.025330489377342E-4
31	0.0010042217482295571	0.0	0.0	1.0144648100614986	6.025330489377342E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCA	35	8.8623044E-4	26.428572	4
GGTATCA	310	0.0	25.66129	1
CTCTATG	60	1.3348636E-6	24.666668	1
GTATTGA	45	0.0038228086	20.555555	1
GCAATAC	45	0.0038228086	20.555555	3
ACCTTAG	45	0.0038228086	20.555555	1
TGATAGA	60	9.226793E-4	18.5	2
TAGGCTC	50	0.0070294193	18.499998	5
TATGCCG	75	2.0643024E-4	17.266666	33
TATACCA	75	2.0643024E-4	17.266666	5
GTATCAA	455	0.0	17.076923	2
GGACCGC	90	4.4378183E-5	16.444445	6
AGTACAG	115	1.2386608E-6	16.086956	5
GCGGGTA	70	0.0025897266	15.857143	23
CTTATAC	720	0.0	15.673611	37
TCTTATA	1135	0.0	14.995595	37
TTCTAAG	75	0.0041000624	14.8	2
CTATGCC	75	0.0041000624	14.8	3
TAATAGA	75	0.0041000624	14.8	4
TATGGGC	75	0.0041000624	14.8	4
>>END_MODULE