FastQCFastQC Report
Fri 10 Feb 2017
ERR1632224.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632224.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201479
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG250.005485722329.5999985
GGTATCA950.027.2631591
TAAAACC358.843919E-426.4285724
GTACTCC358.843919E-426.4285724
CTAGAAC509.042587E-625.8999983
CAACTGC400.00192569323.1250026
GTCTAGA652.6646521E-622.769231
TAGAACT502.6913977E-422.1999994
TCTAGAC502.6913977E-422.1999993
ACTGATC450.003815028120.5555558
TAAAGAC450.003815028120.5555553
GCAATTA450.003815028120.55555517
GTATCAA1409.276846E-1118.52
TGTAGCG609.200819E-418.52
TAGCGCG500.00701525118.4999984
CATCTAA500.00701525118.49999825
CAATTAC500.00701525118.49999818
TCAGGAC500.00701525118.4999983
AACGGAG500.00701525118.49999825
CTACACA500.00701525118.4999984
Produced by FastQC (version 0.11.5)