FastQCFastQC Report
Fri 10 Feb 2017
ERR1632223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456495
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.1182926428547958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT303.596108E-430.8333346
GTAAGTG303.596108E-430.8333345
GGTATCA2800.027.751
ATTAATC450.003822327620.5555553
GTATCAA4050.018.7283952
TTACCAC609.225187E-418.53
AGATAAC500.007028545318.4999981
CTATACA500.007028545318.4999984
TTCGATT500.007028545318.49999836
TCTTATA8300.017.60843537
CTACACT904.436515E-516.4444454
TACACAT1251.6511694E-716.2799995
ACTGATC700.002589284215.8571428
ATGCCGT855.3553557E-415.23529434
AGGTCGG750.004099367714.810
CTTATAC5050.014.65346637
TATGCCG800.00629095413.87533
CCGTCTT800.00629095413.87537
GCCGTCT800.00629095413.87536
AACAACC850.009393373513.05882428
Produced by FastQC (version 0.11.5)