##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632223.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	456495
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7709175346937	34.0	31.0	34.0	31.0	34.0
2	32.93274844193255	34.0	31.0	34.0	31.0	34.0
3	32.94466971160692	34.0	33.0	34.0	31.0	34.0
4	36.38212247669745	37.0	37.0	37.0	35.0	37.0
5	36.29648955629306	37.0	37.0	37.0	35.0	37.0
6	36.332218315644205	37.0	37.0	37.0	35.0	37.0
7	36.31701332982836	37.0	37.0	37.0	35.0	37.0
8	36.2968619590576	37.0	37.0	37.0	35.0	37.0
9	38.086390869560454	39.0	39.0	39.0	37.0	39.0
10	38.01872090603402	39.0	38.0	39.0	35.0	39.0
11	38.12930043045378	39.0	39.0	39.0	37.0	39.0
12	38.09481374385262	39.0	38.0	39.0	37.0	39.0
13	38.11491254011545	39.0	39.0	39.0	37.0	39.0
14	39.52224668397244	41.0	39.0	41.0	37.0	41.0
15	39.54893481856318	41.0	39.0	41.0	37.0	41.0
16	39.43765430070428	41.0	39.0	41.0	37.0	41.0
17	39.48515536862397	41.0	39.0	41.0	37.0	41.0
18	39.500217965147485	41.0	39.0	41.0	37.0	41.0
19	39.559701639667466	41.0	39.0	41.0	37.0	41.0
20	39.53183715046167	41.0	39.0	41.0	37.0	41.0
21	39.51091468690785	41.0	39.0	41.0	37.0	41.0
22	39.49074360069661	41.0	39.0	41.0	37.0	41.0
23	39.45365666655714	41.0	39.0	41.0	37.0	41.0
24	39.40384451089278	41.0	39.0	41.0	37.0	41.0
25	39.34270035816384	40.0	39.0	41.0	36.0	41.0
26	39.266546183419315	40.0	39.0	41.0	36.0	41.0
27	39.20278644892058	40.0	39.0	41.0	36.0	41.0
28	39.15765780567148	40.0	39.0	41.0	36.0	41.0
29	39.022431790052465	40.0	39.0	41.0	36.0	41.0
30	38.9850118840294	40.0	39.0	41.0	35.0	41.0
31	38.92601890491681	40.0	38.0	41.0	35.0	41.0
32	38.86451330244581	40.0	38.0	41.0	35.0	41.0
33	38.742211853360935	40.0	38.0	41.0	35.0	41.0
34	38.74686907852222	40.0	38.0	41.0	35.0	41.0
35	38.73648123199597	40.0	38.0	41.0	35.0	41.0
36	38.71731125204	40.0	38.0	41.0	35.0	41.0
37	38.696539940196494	40.0	38.0	41.0	35.0	41.0
38	38.64145938071611	40.0	38.0	41.0	35.0	41.0
39	38.46826142674071	40.0	38.0	41.0	35.0	41.0
40	38.48980383136727	40.0	38.0	41.0	35.0	41.0
41	38.50268239520696	40.0	38.0	41.0	35.0	41.0
42	38.39153769482689	40.0	38.0	41.0	34.0	41.0
43	37.569782801564095	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	5.0
18	4.0
19	5.0
20	28.0
21	41.0
22	87.0
23	156.0
24	248.0
25	406.0
26	569.0
27	862.0
28	1262.0
29	1799.0
30	2593.0
31	3610.0
32	5100.0
33	6917.0
34	10493.0
35	15856.0
36	25563.0
37	47065.0
38	111342.0
39	222482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.646031172301996	17.926154722395644	12.467825496445744	26.959988608856616
2	19.55158325940043	19.545011445908496	33.768606446949036	27.134798847742037
3	20.442940229356292	21.007020887413884	28.92408460114569	29.625954282084145
4	15.686918805244307	15.273551736601714	32.99882802659394	36.04070143156004
5	16.625373771892356	34.03301240977448	33.113177581353575	16.228436236979594
6	36.56053187877195	32.931795528976224	15.063034644410125	15.444637947841708
7	30.998367999649503	28.58366466226355	20.225851323672767	20.19211601441418
8	28.08092093013067	32.21349631430793	19.212258622766953	20.493324132794445
9	27.207965037952224	13.900699898136889	18.900316542349863	39.991018521561024
10	18.003264000700995	25.54266749909638	32.275490421581836	24.178578078620795
11	37.44772670018291	20.47076090647214	20.392118205018676	21.68939418832627
12	22.9798792976922	24.285479578089575	27.943789088598997	24.790852035619228
13	32.38830655319335	18.706448044337833	22.39695944095773	26.50828596151108
14	24.04473214383509	19.526829428580818	23.604639700325304	32.82379872725878
15	26.543554693917788	26.070822243398066	21.055652307254185	26.32997075542996
16	26.527782341537147	24.55864795890426	22.830041950076126	26.08352774948247
17	24.93565099289149	25.04167625056134	24.21757083867293	25.805101917874236
18	25.373771892353698	24.097306651770555	24.73323913733995	25.7956823185358
19	26.02788639525077	24.065542886559545	24.832911641967602	25.073659076222082
20	26.363925125138284	23.969813470027056	24.517902715254277	25.14835868958039
21	26.56020328809735	23.8736459325951	23.74680993220079	25.81934084710676
22	26.858782681080847	23.98317615746065	23.61931675045729	25.53872441100122
23	26.45461615132696	23.859626063812307	23.738923756010472	25.94683402885026
24	26.07805123823919	24.08153429938992	23.67539622558845	26.16501823678244
25	26.267757587706324	23.81406149026824	24.00661562558188	25.911565296443555
26	25.615614628856832	23.896209158917404	24.318995826898433	26.16918038532733
27	25.549239312588305	24.10344034436303	24.332577574781762	26.014742768266903
28	25.84409467792637	23.878684322938916	24.335644421077994	25.94157657805671
29	25.4519764729077	24.037941269893427	24.593697630861236	25.916384626337635
30	25.522513937721115	24.341559053220735	24.291832331131776	25.84409467792637
31	25.82437923745057	24.04823711103079	23.985585822407693	26.141797829110942
32	24.996111677017275	24.131480081928608	24.597640718956395	26.274767522097726
33	25.0526292730479	23.883065531933536	24.899067897786395	26.165237297232167
34	25.559316093275942	23.19674914292599	24.927545756251437	26.316389007546633
35	24.939594080986648	23.27232499808322	25.69228578626272	26.09579513466741
36	24.997864160615123	23.150527388032728	25.44978586841039	26.401822582941765
37	24.484386466445414	22.175270265829855	26.37925935661946	26.96108391110527
38	23.922277352435405	22.336936877731407	27.363278896811575	26.377506873021613
39	23.737609393312084	21.920502962792582	27.978400639656513	26.363487004238817
40	23.269258151786985	21.58074020525964	29.075455371909882	26.0745462710435
41	22.6488789581485	21.513707707641924	29.22310211502864	26.61431121918093
42	21.918969539644465	21.73977809176442	29.67480476237418	26.666447606216938
43	21.637695922189728	21.24294899177428	30.06429424199608	27.055060844039915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	9.5
2	18.0
3	47.5
4	77.0
5	77.0
6	96.0
7	115.0
8	116.0
9	117.0
10	179.0
11	241.0
12	241.0
13	402.0
14	563.0
15	886.5
16	1210.0
17	1214.5
18	1219.0
19	1219.0
20	1521.5
21	1824.0
22	1961.5
23	2099.0
24	2595.5
25	3092.0
26	3092.0
27	3890.0
28	4688.0
29	5686.0
30	6684.0
31	8105.5
32	9527.0
33	9527.0
34	11264.0
35	13001.0
36	14642.5
37	16284.0
38	17985.0
39	19686.0
40	19686.0
41	21600.0
42	23514.0
43	25410.5
44	27307.0
45	28772.5
46	30238.0
47	30238.0
48	31236.5
49	32235.0
50	33366.0
51	34497.0
52	35243.0
53	35989.0
54	35989.0
55	36271.5
56	36554.0
57	35318.5
58	34083.0
59	32619.5
60	31156.0
61	31156.0
62	28539.5
63	25923.0
64	23135.0
65	20347.0
66	17774.0
67	15201.0
68	15201.0
69	12819.5
70	10438.0
71	8747.5
72	7057.0
73	5867.0
74	4677.0
75	4677.0
76	3759.5
77	2842.0
78	2308.0
79	1774.0
80	1441.0
81	1108.0
82	1108.0
83	838.5
84	569.0
85	449.0
86	329.0
87	223.5
88	118.0
89	118.0
90	88.0
91	58.0
92	40.5
93	23.0
94	16.0
95	9.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	456495.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.9012687490143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.10494450263282	73.7970927532515
2	6.54515011641333	10.721121973740209
3	1.4778938545968126	3.6312414530355066
4	0.6373935774809125	2.0881337075263957
5	0.3539198850601777	1.4493243810966931
6	0.20508211394242704	1.0077891197768811
7	0.1338383049444577	0.7673068887517993
8	0.09123533386057914	0.597783167833707
9	0.07894092214962126	0.5818825512244032
>10	0.3673152768355305	4.667217924915832
>50	0.0016072921299047358	0.08315213446157602
>100	0.002410937958085438	0.4894786178000986
>500	2.6788199534282024E-4	0.11847532658541043
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.1182926428547958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.190604497311033E-4
2	0.0	0.0	0.0	0.0	2.190604497311033E-4
3	0.0	0.0	0.0	0.0	2.190604497311033E-4
4	0.0	0.0	0.0	0.0	2.190604497311033E-4
5	0.0	0.0	0.0	0.0	2.190604497311033E-4
6	0.0	0.0	0.0	0.0	2.190604497311033E-4
7	0.0	0.0	0.0	0.0	2.190604497311033E-4
8	0.0	0.0	0.0	0.0	2.190604497311033E-4
9	0.0	0.0	0.0	0.0	2.190604497311033E-4
10	0.0	0.0	0.0	0.0	2.190604497311033E-4
11	0.0	0.0	0.0	0.0	2.190604497311033E-4
12	0.0	0.0	0.0	0.0	2.190604497311033E-4
13	0.0	0.0	0.0	0.0	2.190604497311033E-4
14	0.0	0.0	0.0	0.0	2.190604497311033E-4
15	0.0	0.0	0.0	0.0	2.190604497311033E-4
16	0.0	0.0	0.0	4.381208994622066E-4	2.190604497311033E-4
17	0.0	0.0	0.0	4.381208994622066E-4	2.190604497311033E-4
18	0.0	0.0	0.0	4.381208994622066E-4	2.190604497311033E-4
19	0.0	0.0	0.0	8.762417989244132E-4	2.190604497311033E-4
20	0.0	0.0	0.0	8.762417989244132E-4	2.190604497311033E-4
21	0.0	0.0	0.0	0.0010953022486555166	2.190604497311033E-4
22	0.0	0.0	0.0	0.0019715440475799296	2.190604497311033E-4
23	0.0	0.0	0.0	0.002847785846504343	2.190604497311033E-4
24	0.0	0.0	0.0	0.003724027645428756	2.190604497311033E-4
25	0.0	0.0	0.0	0.005914632142739789	2.190604497311033E-4
26	0.0	0.0	0.0	0.012048324735210681	2.190604497311033E-4
27	0.0	0.0	0.0	0.041621485448909624	2.190604497311033E-4
28	0.0	2.190604497311033E-4	0.0	0.1266169399445777	2.190604497311033E-4
29	0.0	2.190604497311033E-4	0.0	0.24425240145018018	2.190604497311033E-4
30	0.0	2.190604497311033E-4	0.0	0.4002234416587257	2.190604497311033E-4
31	0.0	2.190604497311033E-4	0.0	0.8933285140034393	2.190604497311033E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	30	3.596108E-4	30.833334	6
GTAAGTG	30	3.596108E-4	30.833334	5
GGTATCA	280	0.0	27.75	1
ATTAATC	45	0.0038223276	20.555555	3
GTATCAA	405	0.0	18.728395	2
TTACCAC	60	9.225187E-4	18.5	3
AGATAAC	50	0.0070285453	18.499998	1
CTATACA	50	0.0070285453	18.499998	4
TTCGATT	50	0.0070285453	18.499998	36
TCTTATA	830	0.0	17.608435	37
CTACACT	90	4.436515E-5	16.444445	4
TACACAT	125	1.6511694E-7	16.279999	5
ACTGATC	70	0.0025892842	15.857142	8
ATGCCGT	85	5.3553557E-4	15.235294	34
AGGTCGG	75	0.0040993677	14.8	10
CTTATAC	505	0.0	14.653466	37
TATGCCG	80	0.006290954	13.875	33
CCGTCTT	80	0.006290954	13.875	37
GCCGTCT	80	0.006290954	13.875	36
AACAACC	85	0.0093933735	13.058824	28
>>END_MODULE