FastQCFastQC Report
Fri 10 Feb 2017
ERR1632222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018227
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA22200.21802603938021678No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC22160.21763319966962177No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18190.17864385839306954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.1368064292147036No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG13870.13621716964881112No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC13310.13071741370048132No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13070.12836037543691142No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA12880.12649438681158523No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC12150.11932506209322676No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12090.11873580252733427No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG11730.1152002451319794No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT11470.11264678701311201No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC11440.11235215723016576No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA10990.10793271048597218No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA10770.10577209207769976No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA10470.10282579424823737No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA10230.10046875598466747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.027.751
CCATTAG450.003825513220.5555551
CCGCATA555.142539E-420.18181831
GCTTAGG6000.019.1166671
TAGGACA6500.019.0692314
CTTAGGA6350.018.9370062
ATTACTC609.235828E-418.518
TATGCCG1156.402843E-817.69565233
TTAGGAC6950.017.5683463
TCGCCAT5650.017.35398313
GCACATC6850.017.01459915
TCTTATA14650.016.79522337
GTCTAGT904.4451557E-516.4444451
CCGTCTT1251.6570448E-716.27999937
AGAGCAC7050.016.26950312
AGCACAT7000.016.12142814
CTTATAC8750.016.06857137
CGGGTAA700.002592221115.85714224
TAACCCG700.002592221115.85714228
AGGACAC8100.015.5308645
Produced by FastQC (version 0.11.5)