##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632222.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018227
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80645573138406	34.0	31.0	34.0	31.0	34.0
2	32.96763688254191	34.0	33.0	34.0	31.0	34.0
3	32.96737662623364	34.0	33.0	34.0	31.0	34.0
4	36.402487853887195	37.0	37.0	37.0	35.0	37.0
5	36.32387669940004	37.0	37.0	37.0	35.0	37.0
6	36.35422062074567	37.0	37.0	37.0	35.0	37.0
7	36.34570189162142	37.0	37.0	37.0	35.0	37.0
8	36.329038613197255	37.0	37.0	37.0	35.0	37.0
9	38.107041946442195	39.0	39.0	39.0	37.0	39.0
10	38.05573806233777	39.0	38.0	39.0	37.0	39.0
11	38.1603061007025	39.0	39.0	39.0	37.0	39.0
12	38.14085562453166	39.0	39.0	39.0	37.0	39.0
13	38.15204959208506	39.0	39.0	39.0	37.0	39.0
14	39.57775034447132	41.0	39.0	41.0	37.0	41.0
15	39.61453389077288	41.0	39.0	41.0	37.0	41.0
16	39.49297749912348	41.0	39.0	41.0	37.0	41.0
17	39.544862785999584	41.0	39.0	41.0	37.0	41.0
18	39.55582596022302	41.0	39.0	41.0	37.0	41.0
19	39.624869503558635	41.0	39.0	41.0	37.0	41.0
20	39.61275432688389	41.0	39.0	41.0	37.0	41.0
21	39.57838969110031	41.0	39.0	41.0	37.0	41.0
22	39.57392801408723	41.0	39.0	41.0	37.0	41.0
23	39.53796648488009	41.0	39.0	41.0	37.0	41.0
24	39.49594049264064	41.0	39.0	41.0	37.0	41.0
25	39.43655098519289	41.0	39.0	41.0	37.0	41.0
26	39.36901300004812	41.0	39.0	41.0	37.0	41.0
27	39.30373384323928	40.0	39.0	41.0	36.0	41.0
28	39.256701108888294	40.0	39.0	41.0	36.0	41.0
29	39.12624689779391	40.0	39.0	41.0	36.0	41.0
30	39.09914586825924	40.0	39.0	41.0	36.0	41.0
31	39.03096853648548	40.0	39.0	41.0	35.0	41.0
32	38.974806207260265	40.0	39.0	41.0	35.0	41.0
33	38.84822539571235	40.0	38.0	41.0	35.0	41.0
34	38.8592337465025	40.0	38.0	41.0	35.0	41.0
35	38.85477108739014	40.0	38.0	41.0	35.0	41.0
36	38.83657180569755	40.0	38.0	41.0	35.0	41.0
37	38.82520695287004	40.0	38.0	41.0	35.0	41.0
38	38.76170932414874	40.0	38.0	41.0	35.0	41.0
39	38.590170954021055	40.0	38.0	41.0	35.0	41.0
40	38.61823738714452	40.0	38.0	41.0	35.0	41.0
41	38.62964348814164	40.0	38.0	41.0	35.0	41.0
42	38.52766524556901	40.0	38.0	41.0	35.0	41.0
43	37.72153458904547	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	1.0
13	1.0
14	0.0
15	2.0
16	2.0
17	5.0
18	9.0
19	28.0
20	65.0
21	87.0
22	183.0
23	336.0
24	542.0
25	847.0
26	1212.0
27	1780.0
28	2570.0
29	3918.0
30	5372.0
31	7564.0
32	10575.0
33	14358.0
34	21789.0
35	32573.0
36	52518.0
37	99279.0
38	240867.0
39	521741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58529777741113	18.04784198415481	12.781629243773738	26.585230994660325
2	18.24455646923525	20.870591724635077	34.76719827700503	26.117653529124645
3	19.80638894863326	21.848467974233643	30.90911947925168	27.436023597881416
4	15.411003636713621	15.602021945990433	33.36643007895096	35.620544338344985
5	15.31770420544731	35.81146443769415	33.71733415043993	15.15349720641861
6	35.00152716437494	33.84706946486393	15.96647898749493	15.184924383266207
7	29.39874900194161	30.410409466651345	20.606799858970543	19.5840416724365
8	27.22261342510069	33.584554328258825	20.10975941514024	19.083072831500246
9	26.485744337952145	15.120204040945683	18.791683976166414	39.602367644935754
10	17.058376963093693	27.378767210062193	32.57014398557492	22.992711841269188
11	35.88639861249015	21.34278505677025	21.70635820892591	21.0644581218137
12	22.351499223650524	24.89307394127243	28.07812010484892	24.677306730228132
13	30.668897996222842	19.237164207981127	24.31265326886834	25.78128452692769
14	23.259941054401427	20.10730416694902	24.074887034030723	32.55786774461883
15	25.71440356619889	27.299904638160257	21.880975460285377	25.104716335355477
16	26.042424724545704	25.65616507910319	22.933491254896992	25.367918941454114
17	24.873824795453274	26.030835953083155	24.159151151953346	24.936188099510225
18	23.86992291502779	24.883743998145796	25.762231800963832	25.484101285862582
19	25.496868576456922	24.983427074709276	25.880083714142327	23.63962063469148
20	25.495886477180434	24.314421047566015	25.85670975136193	24.33298272389163
21	25.189471502916344	25.483315606441394	24.18095375589137	25.1462591347509
22	25.470155476136462	24.638710228662177	24.319626173731397	25.571508121469964
23	24.773454249396252	25.41692569535084	24.555722839798985	25.253897215453918
24	26.43732684362131	24.638612018734527	24.36411527095628	24.55994586668788
25	25.55451780398673	24.084806236723246	25.02300567555172	25.3376702837383
26	25.41309550817254	24.189006969958566	24.796140742683114	25.60175677918578
27	24.974195341510292	24.477449527462934	24.85290608086409	25.695449050162683
28	24.341428777669417	24.9224387096394	25.235040909345365	25.501091603345817
29	24.823835942280063	25.45846849474626	24.806943834724475	24.910751728249203
30	24.145991021648413	26.19808745986897	24.59107841375253	25.064843104730084
31	25.07751218539677	24.87706572306568	25.361240666374	24.684181425163544
32	24.071155056780068	25.148419753159168	25.32166206553156	25.458763124529206
33	24.4800029855818	24.602568975287436	25.491467030436237	25.425961008694525
34	24.516929918377727	24.513787200692967	25.57131170161467	25.39797117931463
35	24.477842367173526	24.356062056889083	25.518965810177885	25.6471297657595
36	24.214345131291942	24.34221445709061	26.01610446393584	25.427335947681605
37	25.010434804812682	22.614800039676812	26.040558735920378	26.334206419590128
38	23.71317987050039	23.347151470153513	27.553089831638722	25.38657882770738
39	23.58383739578699	22.67136895800249	28.08401270050784	25.660780945702676
40	23.148865626230695	22.096742671329675	29.1333857774347	25.621005925004937
41	22.179140800626975	22.65732493834872	29.562072111621475	25.60146214940284
42	22.60311305828661	21.495206864481105	29.663621176810278	26.238058900422008
43	21.089305233508835	21.901795964946913	30.34814437252204	26.66075442902221
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	37.5
2	52.0
3	115.5
4	179.0
5	179.0
6	249.0
7	319.0
8	347.0
9	375.0
10	532.5
11	690.0
12	690.0
13	1088.0
14	1486.0
15	2642.0
16	3798.0
17	3866.5
18	3935.0
19	3935.0
20	4366.5
21	4798.0
22	5893.5
23	6989.0
24	8135.5
25	9282.0
26	9282.0
27	10748.0
28	12214.0
29	17115.0
30	22016.0
31	23552.5
32	25089.0
33	25089.0
34	28100.5
35	31112.0
36	33465.5
37	35819.0
38	43345.5
39	50872.0
40	50872.0
41	57731.0
42	64590.0
43	62123.5
44	59657.0
45	64431.0
46	69205.0
47	69205.0
48	73349.5
49	77494.0
50	82866.0
51	88238.0
52	93233.0
53	98228.0
54	98228.0
55	85438.5
56	72649.0
57	67551.5
58	62454.0
59	56494.5
60	50535.0
61	50535.0
62	47128.5
63	43722.0
64	40013.5
65	36305.0
66	31836.5
67	27368.0
68	27368.0
69	23576.0
70	19784.0
71	17122.5
72	14461.0
73	12011.5
74	9562.0
75	9562.0
76	7887.5
77	6213.0
78	5147.5
79	4082.0
80	3167.5
81	2253.0
82	2253.0
83	1752.5
84	1252.0
85	945.5
86	639.0
87	460.0
88	281.0
89	281.0
90	211.5
91	142.0
92	96.0
93	50.0
94	30.5
95	11.0
96	11.0
97	7.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1018227.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.117657403856306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.8781357621396	51.072713875136024
2	7.132762472389614	8.290788914268452
3	1.8771082231365297	3.272793978666308
4	0.806451775175984	1.8747635192963832
5	0.4792198552716589	1.3925567684901934
6	0.309078018377314	1.0777734229869322
7	0.22017763664175785	0.8957345918035508
8	0.16548760839575008	0.7694201703462189
9	0.10892133198792028	0.5697227390801082
>10	0.8274949437973661	9.098954302207396
>50	0.08908597355100002	3.591453254883141
>100	0.09264685520314549	11.46683471899917
>500	0.010369647846543747	4.2446998054952205
>1k	0.0030598960858653685	2.381789938340908
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2220	0.21802603938021678	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2216	0.21763319966962177	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1819	0.17864385839306954	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1393	0.1368064292147036	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1387	0.13621716964881112	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1331	0.13071741370048132	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1307	0.12836037543691142	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1288	0.12649438681158523	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1215	0.11932506209322676	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1209	0.11873580252733427	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1173	0.1152002451319794	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1147	0.11264678701311201	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	1144	0.11235215723016576	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1099	0.10793271048597218	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	1077	0.10577209207769976	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	1047	0.10282579424823737	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	1023	0.10046875598466747	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.964198552974926E-4	0.0
11	0.0	0.0	0.0	1.964198552974926E-4	0.0
12	0.0	0.0	0.0	2.946297829462389E-4	0.0
13	0.0	0.0	0.0	2.946297829462389E-4	0.0
14	0.0	0.0	0.0	2.946297829462389E-4	0.0
15	0.0	0.0	0.0	3.928397105949852E-4	0.0
16	0.0	0.0	0.0	4.910496382437315E-4	0.0
17	0.0	9.82099276487463E-5	0.0	5.892595658924778E-4	0.0
18	0.0	9.82099276487463E-5	0.0	7.856794211899704E-4	0.0
19	0.0	9.82099276487463E-5	0.0	7.856794211899704E-4	0.0
20	0.0	9.82099276487463E-5	0.0	9.82099276487463E-4	0.0
21	0.0	9.82099276487463E-5	0.0	0.001276729059433702	9.82099276487463E-5
22	0.0	9.82099276487463E-5	0.0	0.0018659886253261798	9.82099276487463E-5
23	0.0	9.82099276487463E-5	0.0	0.002553458118867404	9.82099276487463E-5
24	0.0	9.82099276487463E-5	0.0	0.0040266070335985985	9.82099276487463E-5
25	0.0	9.82099276487463E-5	0.0	0.005892595658924778	9.82099276487463E-5
26	0.0	9.82099276487463E-5	0.0	0.008937103416035914	9.82099276487463E-5
27	0.0	9.82099276487463E-5	0.0	0.0318200165581938	9.82099276487463E-5
28	0.0	9.82099276487463E-5	0.0	0.10616493178829475	9.82099276487463E-5
29	0.0	9.82099276487463E-5	0.0	0.22470431446033154	9.82099276487463E-5
30	0.0	9.82099276487463E-5	0.0	0.3879292142125479	9.82099276487463E-5
31	0.0	9.82099276487463E-5	0.0	0.8310524077636912	9.82099276487463E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	540	0.0	27.75	1
CCATTAG	45	0.0038255132	20.555555	1
CCGCATA	55	5.142539E-4	20.181818	31
GCTTAGG	600	0.0	19.116667	1
TAGGACA	650	0.0	19.069231	4
CTTAGGA	635	0.0	18.937006	2
ATTACTC	60	9.235828E-4	18.5	18
TATGCCG	115	6.402843E-8	17.695652	33
TTAGGAC	695	0.0	17.568346	3
TCGCCAT	565	0.0	17.353983	13
GCACATC	685	0.0	17.014599	15
TCTTATA	1465	0.0	16.795223	37
GTCTAGT	90	4.4451557E-5	16.444445	1
CCGTCTT	125	1.6570448E-7	16.279999	37
AGAGCAC	705	0.0	16.269503	12
AGCACAT	700	0.0	16.121428	14
CTTATAC	875	0.0	16.068571	37
CGGGTAA	70	0.0025922211	15.857142	24
TAACCCG	70	0.0025922211	15.857142	28
AGGACAC	810	0.0	15.530864	5
>>END_MODULE