FastQCFastQC Report
Fri 10 Feb 2017
ERR1632221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475858
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT250.00549314729.64
GGTATCA1850.029.01
ACCTTAT400.001929533323.1258
GTACACG450.00382256320.5555554
TTTACCC450.00382256320.5555553
TCCGTGT450.00382256320.5555558
CCGTCTT555.13701E-420.18181837
GTATCAA2700.019.870372
CCTTAGG500.007028972318.52
GTCCTAA701.21729296E-418.51
TATGCTT500.007028972318.55
TACTGTG609.2259777E-418.57
GTGTATA609.2259777E-418.513
CGAATTA500.007028972318.515
GTATAGT609.2259777E-418.51
TCTTATA7850.018.14649837
TATGCCG650.001578095317.07692333
TATACTG650.001578095317.0769235
GATACTT1005.8632395E-616.65000214
AGCGCGC700.002589501415.8571428
Produced by FastQC (version 0.11.5)