##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475858
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.816781476827124	34.0	31.0	34.0	31.0	34.0
2	32.980054974383116	34.0	33.0	34.0	31.0	34.0
3	32.98611350444881	34.0	33.0	34.0	31.0	34.0
4	36.42450689071109	37.0	37.0	37.0	35.0	37.0
5	36.33731701473969	37.0	37.0	37.0	35.0	37.0
6	36.371552017618704	37.0	37.0	37.0	35.0	37.0
7	36.36152801886277	37.0	37.0	37.0	35.0	37.0
8	36.34293843961854	37.0	37.0	37.0	35.0	37.0
9	38.142401724884316	39.0	39.0	39.0	37.0	39.0
10	38.093540509984074	39.0	38.0	39.0	37.0	39.0
11	38.187684141067294	39.0	39.0	39.0	37.0	39.0
12	38.1577067108255	39.0	39.0	39.0	37.0	39.0
13	38.17895674760118	39.0	39.0	39.0	37.0	39.0
14	39.60928049964485	41.0	39.0	41.0	37.0	41.0
15	39.63767552505159	41.0	39.0	41.0	37.0	41.0
16	39.53621878795775	41.0	39.0	41.0	37.0	41.0
17	39.575205208276415	41.0	39.0	41.0	37.0	41.0
18	39.584371808396625	41.0	39.0	41.0	37.0	41.0
19	39.63183554757932	41.0	39.0	41.0	37.0	41.0
20	39.60898419276339	41.0	39.0	41.0	37.0	41.0
21	39.583608975786895	41.0	39.0	41.0	37.0	41.0
22	39.574045198357496	41.0	39.0	41.0	37.0	41.0
23	39.543830302317076	41.0	39.0	41.0	37.0	41.0
24	39.49284240256547	41.0	39.0	41.0	37.0	41.0
25	39.433431822098186	41.0	39.0	41.0	37.0	41.0
26	39.36413383824586	41.0	39.0	41.0	37.0	41.0
27	39.30114445906132	40.0	39.0	41.0	36.0	41.0
28	39.25066721585011	40.0	39.0	41.0	36.0	41.0
29	39.133029180974155	40.0	39.0	41.0	36.0	41.0
30	39.09637538929681	40.0	39.0	41.0	36.0	41.0
31	39.03025062098357	40.0	39.0	41.0	35.0	41.0
32	38.97731045816189	40.0	39.0	41.0	35.0	41.0
33	38.86486304737968	40.0	38.0	41.0	35.0	41.0
34	38.863333179225734	40.0	38.0	41.0	35.0	41.0
35	38.84567875290528	40.0	38.0	41.0	35.0	41.0
36	38.826517574570566	40.0	38.0	41.0	35.0	41.0
37	38.79764761756658	40.0	38.0	41.0	35.0	41.0
38	38.736251150553315	40.0	38.0	41.0	35.0	41.0
39	38.58499594416822	40.0	38.0	41.0	35.0	41.0
40	38.59229013697364	40.0	38.0	41.0	35.0	41.0
41	38.60627119855083	40.0	38.0	41.0	35.0	41.0
42	38.49884629448281	40.0	38.0	41.0	35.0	41.0
43	37.68154155231183	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	5.0
19	8.0
20	27.0
21	40.0
22	85.0
23	159.0
24	256.0
25	349.0
26	575.0
27	800.0
28	1098.0
29	1722.0
30	2408.0
31	3263.0
32	4596.0
33	6695.0
34	9820.0
35	15453.0
36	25198.0
37	47165.0
38	115956.0
39	240177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.79007603108491	17.651694413039184	12.45812826515473	27.100101290721177
2	19.345477012049813	19.56255857839944	33.89708694610577	27.19487746344498
3	20.3510290885096	20.813982322457537	29.34762050863913	29.487368080393733
4	15.848845664042635	15.096520390536671	32.7244682237138	36.3301657217069
5	16.74764320448537	34.0179213126605	32.84740405751296	16.387031425341174
6	36.34088320465349	32.98946324323643	15.075926011541258	15.593727540568825
7	30.913844045912857	28.835072647722644	20.095070378138015	20.156012928226488
8	28.017391742914903	32.38297979649391	19.238092035859435	20.361536424731746
9	27.0063758516196	14.055873811094907	18.724493441320732	40.21325689596476
10	17.801318880842604	25.78857558347238	32.14194150355779	24.26816403212723
11	37.25565189615389	20.505907224424096	20.603415304565647	21.635025574856364
12	22.86774626043904	23.86909540240996	27.942369362288748	25.320788974862246
13	31.992317035754365	18.533259922077594	22.979544317842716	26.49487872432532
14	23.904189905391945	19.485434730528855	23.615658452731697	32.9947169113475
15	26.689474591159545	26.10610728410576	20.775735618608913	26.428682506125778
16	26.57851712065364	24.968162771246885	22.379575419557934	26.073744688541538
17	25.30965119846677	25.06377953086845	24.142496290910316	25.484072979754462
18	25.2785494832492	24.00716180036902	24.679421171862195	26.034867544519585
19	26.38497198744163	24.154054360754678	24.46801356707253	24.99296008473116
20	26.63105380176439	23.73607252583754	24.279511955247155	25.353361717150914
21	26.297130656624457	24.132199101412606	23.43808447057736	26.132585771385582
22	26.563596703218188	24.007582093817902	23.567114559385363	25.861706643578547
23	26.151078683136564	23.98425580740473	23.579303069403057	26.28536244005565
24	26.349036897561877	23.894313009343122	23.554715902643224	26.201934190451777
25	26.580408441173624	23.25441623341417	23.83063855183689	26.334536773575312
26	26.016164486044158	23.84513867582346	23.793022288161595	26.345674549970788
27	25.90037364087606	23.983205073782514	23.760659692597372	26.355761592744052
28	25.515594988420915	24.10445973378613	24.181793728381155	26.198151549411797
29	25.49563104959883	24.324483354277955	24.30178750803811	25.8780980880851
30	25.600284118371448	24.578971037578437	23.79638463575268	26.02436020829743
31	25.896590999836082	24.164351550252388	23.886747727263174	26.052309722648353
32	25.33907173988879	23.995813877249095	24.53168802457876	26.13342635828335
33	25.37122418872857	23.62763681602495	24.591579841045018	26.40955915420146
34	25.725951859588363	23.31577907695153	24.610072752796004	26.348196310664108
35	25.36512993371972	23.16006035413926	25.280440803769196	26.194368908371825
36	25.192599472952015	22.97681241042496	25.301035182764608	26.52955293385842
37	25.241773806471677	21.65772142109621	26.188064506638536	26.912440265793574
38	24.305780295802528	22.249074303678828	26.824809081700845	26.6203363188178
39	23.909863866951905	22.06750753376007	27.62462751493093	26.398001084357098
40	23.528237415363407	21.437487653879938	28.648462356417248	26.3858125743394
41	22.947391869002935	21.640699536416324	28.94266777063746	26.46924082394328
42	22.664114084453765	21.56504671561684	29.219641153453342	26.55119804647605
43	22.05468858356905	21.542981309550328	29.491571014882588	26.91075909199803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	7.5
2	5.0
3	20.0
4	35.0
5	35.0
6	40.5
7	46.0
8	54.0
9	62.0
10	96.5
11	131.0
12	131.0
13	223.0
14	315.0
15	546.0
16	777.0
17	873.0
18	969.0
19	969.0
20	1359.0
21	1749.0
22	2206.0
23	2663.0
24	3323.0
25	3983.0
26	3983.0
27	4509.0
28	5035.0
29	6345.0
30	7655.0
31	8983.0
32	10311.0
33	10311.0
34	11841.5
35	13372.0
36	15238.5
37	17105.0
38	18884.0
39	20663.0
40	20663.0
41	22696.5
42	24730.0
43	25541.5
44	26353.0
45	28152.0
46	29951.0
47	29951.0
48	31328.5
49	32706.0
50	34982.5
51	37259.0
52	38329.5
53	39400.0
54	39400.0
55	37768.0
56	36136.0
57	35013.0
58	33890.0
59	32246.0
60	30602.0
61	30602.0
62	29134.5
63	27667.0
64	24607.0
65	21547.0
66	19039.0
67	16531.0
68	16531.0
69	14262.5
70	11994.0
71	10247.5
72	8501.0
73	7044.5
74	5588.0
75	5588.0
76	4493.5
77	3399.0
78	2803.5
79	2208.0
80	1705.5
81	1203.0
82	1203.0
83	948.5
84	694.0
85	517.5
86	341.0
87	256.5
88	172.0
89	172.0
90	119.5
91	67.0
92	45.0
93	23.0
94	13.5
95	4.0
96	4.0
97	4.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	475858.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.20883699590065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.00414387929796	66.79102842094412
2	6.072302294461954	9.012369823191213
3	1.4866437558230876	3.309663124405471
4	0.6504475619300726	1.9307582839059898
5	0.3917852289380414	1.4536963095832367
6	0.23060156946255175	1.026760456754722
7	0.19059236946058275	0.9900546654574001
8	0.13524771106196404	0.8029260275412765
9	0.10841065526620655	0.724052578074183
>10	0.6573533753923141	9.070163771905081
>50	0.05257743520662553	2.63101814858667
>100	0.01989416369860422	2.2575083896506762
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	2.101467244430061E-4	0.0
18	0.0	0.0	0.0	2.101467244430061E-4	0.0
19	0.0	0.0	0.0	2.101467244430061E-4	0.0
20	0.0	0.0	0.0	2.101467244430061E-4	0.0
21	0.0	0.0	0.0	4.202934488860122E-4	0.0
22	0.0	0.0	0.0	4.202934488860122E-4	0.0
23	0.0	0.0	0.0	0.0016811737955440488	0.0
24	0.0	0.0	0.0	0.0035724943155311037	0.0
25	0.0	0.0	0.0	0.004623227937746134	0.0
26	0.0	0.0	0.0	0.010087042773264294	0.0
27	0.0	0.0	0.0	0.030681421768678892	0.0
28	0.0	0.0	0.0	0.11263864430145128	0.0
29	0.0	0.0	0.0	0.23179183706063575	0.0
30	0.0	0.0	0.0	0.377843810548525	0.0
31	0.0	0.0	0.0	0.8096953292789025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACT	25	0.005493147	29.6	4
GGTATCA	185	0.0	29.0	1
ACCTTAT	40	0.0019295333	23.125	8
GTACACG	45	0.003822563	20.555555	4
TTTACCC	45	0.003822563	20.555555	3
TCCGTGT	45	0.003822563	20.555555	8
CCGTCTT	55	5.13701E-4	20.181818	37
GTATCAA	270	0.0	19.87037	2
CCTTAGG	50	0.0070289723	18.5	2
GTCCTAA	70	1.21729296E-4	18.5	1
TATGCTT	50	0.0070289723	18.5	5
TACTGTG	60	9.2259777E-4	18.5	7
GTGTATA	60	9.2259777E-4	18.5	13
CGAATTA	50	0.0070289723	18.5	15
GTATAGT	60	9.2259777E-4	18.5	1
TCTTATA	785	0.0	18.146498	37
TATGCCG	65	0.0015780953	17.076923	33
TATACTG	65	0.0015780953	17.076923	5
GATACTT	100	5.8632395E-6	16.650002	14
AGCGCGC	70	0.0025895014	15.857142	8
>>END_MODULE