FastQCFastQC Report
Fri 10 Feb 2017
ERR1632220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265481
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAG200.001839387337.013
ATATATT250.00548882629.64
TATAAGA250.00548882629.62
CAATACT250.00548882629.64
GTATTAG706.542905E-926.4285721
ACAGTCC400.00192729823.1258
TCTAGGT400.00192729823.1253
GTATAAG400.00192729823.1251
AAGATAC450.003818177420.5555553
TACTGTG500.007020985718.57
GGTATCA801.6090513E-518.51
GTATCAA1700.018.4999981
GCTCTAG700.002585457415.8571431
GTGCTCC700.002585457415.8571439
TATACAC1001.0883411E-414.837
CTTGCGC750.0040933614.7999993
CAACGCA2700.013.7037044
TATCAAC2900.013.3965511
TCCCAAG850.009379839513.0588232
TTATACA1000.001826038812.95000136
Produced by FastQC (version 0.11.5)