ERR1632219.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632219.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49854
Sequences flagged as poor quality	0
Sequence length	43
%GC	52

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	283	0.5676575600754202	TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	133	0.2667789946644201	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	120	0.2407028523288001	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	118	0.2366911381233201	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	89	0.17852128214386007	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	85	0.17049785373290008	RNA PCR Primer, Index 3 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAGAG	35	2.3431247E-5	31.714287	5
CTTGTTC	25	0.005446675	29.599998	3
TATACAC	45	3.908106E-6	28.777777	3
TTATACA	50	8.865514E-6	25.899998	2
CCGTCTT	45	1.2985778E-4	24.666666	37
GCCGTCT	45	1.2985778E-4	24.666666	36
ATACACA	45	1.2985778E-4	24.666666	4
GGAGAGA	45	0.0037754646	20.555555	6
TGCCGTC	55	5.0488824E-4	20.181818	35
ATGCCGT	60	9.068987E-4	18.5	34
TCCTGTC	50	0.0069431947	18.499998	35
CACATCT	50	0.0069431947	18.499998	7
TACACAT	50	0.0069431947	18.499998	5
TATGCCG	65	0.001551461	17.076921	33
CTTATAC	85	5.237479E-4	15.235294	1
CGTATGC	75	0.004031643	14.8	31
TCTTATA	75	0.004031643	14.8	36
GTATGCC	75	0.004031643	14.8	32
TCGTATG	80	0.006187879	13.875001	30
CTCGTAT	85	0.009240743	13.058824	29