##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632219.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49854
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7916516227384	34.0	31.0	34.0	31.0	34.0
2	32.959481686524654	34.0	33.0	34.0	31.0	34.0
3	32.96228988646849	34.0	33.0	34.0	31.0	34.0
4	36.39461226782204	37.0	37.0	37.0	35.0	37.0
5	36.3123721266097	37.0	37.0	37.0	35.0	37.0
6	36.341416937457375	37.0	37.0	37.0	35.0	37.0
7	36.322040357844905	37.0	37.0	37.0	35.0	37.0
8	36.31542102940587	37.0	37.0	37.0	35.0	37.0
9	38.120391543306454	39.0	39.0	39.0	37.0	39.0
10	38.04390821197898	39.0	38.0	39.0	35.0	39.0
11	38.1685922894853	39.0	39.0	39.0	37.0	39.0
12	38.12524571749508	39.0	38.0	39.0	37.0	39.0
13	38.162033136759334	39.0	39.0	39.0	37.0	39.0
14	39.559493721667266	41.0	39.0	41.0	37.0	41.0
15	39.58877923536727	41.0	39.0	41.0	37.0	41.0
16	39.468387692060816	41.0	39.0	41.0	37.0	41.0
17	39.50970834837726	41.0	39.0	41.0	37.0	41.0
18	39.523628996670276	41.0	39.0	41.0	37.0	41.0
19	39.58908011393268	41.0	39.0	41.0	37.0	41.0
20	39.549263850443296	41.0	39.0	41.0	37.0	41.0
21	39.52740000802343	41.0	39.0	41.0	37.0	41.0
22	39.52060015244514	41.0	39.0	41.0	37.0	41.0
23	39.49051229590404	41.0	39.0	41.0	37.0	41.0
24	39.4459020339391	41.0	39.0	41.0	37.0	41.0
25	39.364504352709915	40.0	39.0	41.0	37.0	41.0
26	39.28653668712641	40.0	39.0	41.0	36.0	41.0
27	39.233020419625305	40.0	39.0	41.0	36.0	41.0
28	39.18788863481365	40.0	39.0	41.0	36.0	41.0
29	39.07999358125727	40.0	39.0	41.0	36.0	41.0
30	39.00328960564849	40.0	39.0	41.0	35.0	41.0
31	38.95849881654431	40.0	39.0	41.0	35.0	41.0
32	38.90835238897581	40.0	38.0	41.0	35.0	41.0
33	38.75622818630401	40.0	38.0	41.0	35.0	41.0
34	38.80771853813135	40.0	38.0	41.0	35.0	41.0
35	38.77997753440045	40.0	38.0	41.0	35.0	41.0
36	38.75428250491435	40.0	38.0	41.0	35.0	41.0
37	38.75059172784531	40.0	38.0	41.0	35.0	41.0
38	38.70194969310386	40.0	38.0	41.0	35.0	41.0
39	38.514141292574315	40.0	38.0	41.0	35.0	41.0
40	38.53827175352028	40.0	38.0	41.0	35.0	41.0
41	38.53748946925021	40.0	38.0	41.0	35.0	41.0
42	38.441268504031775	40.0	38.0	41.0	35.0	41.0
43	37.624684077506316	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	4.0
22	8.0
23	14.0
24	23.0
25	47.0
26	62.0
27	78.0
28	123.0
29	182.0
30	274.0
31	360.0
32	526.0
33	739.0
34	1115.0
35	1740.0
36	2705.0
37	5036.0
38	12472.0
39	24344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66056886107434	17.77189393027641	12.354073895775665	27.213463312873593
2	20.064588598708227	19.70554017731777	32.811810486620935	27.418060737353073
3	20.35142616440005	20.98126529466041	29.243390700846472	29.423917840093072
4	15.741966542303526	15.082039555502066	32.438721065511295	36.73727283668312
5	16.676695952180367	34.25001002928552	32.74160548802503	16.331688530509087
6	36.95992297508725	32.66538291812091	14.977734986159586	15.396959120632244
7	31.08075580695631	28.46712400208609	19.71757531993421	20.734544871023388
8	28.657680426846394	31.54410879768925	18.983431620331366	20.81477915513299
9	26.99281903157219	13.563605728727884	18.106872066433986	41.33670317326594
10	18.38368034661211	24.85457536005135	32.25017049785373	24.51157379548281
11	38.083202952621654	19.71757531993421	19.968307457776707	22.23091426966743
12	23.46251053074979	24.08031451839371	26.916596461668068	25.54057848918843
13	33.19292333614153	17.962450355036708	21.627151281742687	27.217475027079068
14	24.976932643318488	19.256228186304007	22.61403297629077	33.15280619408673
15	26.73406346531873	25.311910779476072	20.30729730813977	27.64672844706543
16	27.55847073454487	23.70521924018133	21.93605327556465	26.80025674970915
17	25.39816263489389	24.96890921490753	23.45849881654431	26.17442933365427
18	26.176435190757008	22.836683114694907	23.76539495326353	27.22148674128455
19	26.86845589120231	23.60091467083885	23.68114895494845	25.84948048301039
20	26.61170618205159	23.04529225337987	24.206683515866327	26.13631804870221
21	27.74902715930517	23.298030248325112	22.57391583423597	26.37902675813375
22	27.79516187266819	23.60091467083885	22.42347655153047	26.18044690496249
23	27.28166245436675	23.39230553215389	22.42748826573595	26.89854374774341
24	27.905484013318894	22.82665382918121	22.57993340554419	26.68792875195571
25	27.014883459702332	23.25791310627031	23.18369639346893	26.543507040558428
26	26.948690175311913	23.66710795522927	23.02322782524973	26.36097404420909
27	26.38705018654471	23.76539495326353	23.298030248325112	26.549524611866648
28	25.92570305291451	23.56681510009227	23.98403337746219	26.52344846953103
29	26.75813375055161	23.19171982187989	23.37224696112649	26.677899466442014
30	25.775263770209012	23.58887952822241	24.23476551530469	26.40109118626389
31	26.70196975167489	23.85766437998957	22.51173426405103	26.928631604284508
32	26.449231756729652	23.82958238055121	23.63902595579091	26.082159906928233
33	25.315922493681548	23.62097324186625	24.38520479801019	26.677899466442014
34	26.25265776066113	22.99915754001685	24.42933365427047	26.31885104505155
35	25.502467204236368	22.70429654591407	25.35202792153087	26.441208328318687
36	25.697035343202153	22.95302282665383	24.19264251614715	27.15729931399687
37	25.33397520760621	21.93605327556465	25.19757692461989	27.532394592209254
38	24.880651502386968	22.04035784490713	26.19849961888715	26.88049103381875
39	25.05315521322261	21.75352027921531	26.479319613270754	26.71400489429133
40	23.89176395073615	21.14775143418783	28.45107714526417	26.50940746981185
41	23.901793236249848	20.98728286596863	27.73699201668873	27.37393188109279
42	23.105467966462072	20.909054438961768	28.729891282545033	27.255586312031134
43	22.53981626348939	20.17691659646167	29.57034540859309	27.712921731455854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	3.5
7	4.0
8	3.0
9	2.0
10	3.0
11	4.0
12	4.0
13	13.0
14	22.0
15	30.0
16	38.0
17	39.0
18	40.0
19	40.0
20	74.0
21	108.0
22	111.5
23	115.0
24	145.0
25	175.0
26	175.0
27	240.5
28	306.0
29	389.5
30	473.0
31	592.5
32	712.0
33	712.0
34	912.5
35	1113.0
36	1339.0
37	1565.0
38	1762.5
39	1960.0
40	1960.0
41	2212.0
42	2464.0
43	2664.0
44	2864.0
45	3139.5
46	3415.0
47	3415.0
48	3571.5
49	3728.0
50	3942.5
51	4157.0
52	4167.0
53	4177.0
54	4177.0
55	4353.5
56	4530.0
57	4298.5
58	4067.0
59	3787.0
60	3507.0
61	3507.0
62	3198.0
63	2889.0
64	2620.5
65	2352.0
66	2058.0
67	1764.0
68	1764.0
69	1463.0
70	1162.0
71	1001.5
72	841.0
73	664.0
74	487.0
75	487.0
76	394.0
77	301.0
78	263.0
79	225.0
80	184.5
81	144.0
82	144.0
83	111.5
84	79.0
85	56.0
86	33.0
87	23.0
88	13.0
89	13.0
90	11.0
91	9.0
92	6.5
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	49854.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.91455048742327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.26448935885423	84.75749187627873
2	4.006603310578682	7.205038713042083
3	0.914647749074198	2.467204236370201
4	0.36362825146120553	1.3078188309864804
5	0.18962209432026056	0.8524892686645004
6	0.11377325659215633	0.6137922734384402
7	0.05577120421184134	0.35102499297950013
8	0.02677017802168384	0.19256228186304009
9	0.011154240842368268	0.09026356962330004
>10	0.04015526703252577	0.5014642756850002
>50	0.004461696336947308	0.34901913587676014
>100	0.008923392673894616	1.3118305451919605
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	283	0.5676575600754202	TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	133	0.2667789946644201	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	120	0.2407028523288001	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	118	0.2366911381233201	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	89	0.17852128214386007	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	85	0.17049785373290008	RNA PCR Primer, Index 3 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0020058571027400007	0.0
25	0.0	0.0	0.0	0.0020058571027400007	0.0
26	0.0	0.0	0.0	0.0020058571027400007	0.0
27	0.0	0.0	0.0	0.03209371364384001	0.0
28	0.0	0.0	0.0	0.08625185541782003	0.0
29	0.0	0.0	0.0	0.17450956793838007	0.0
30	0.0	0.0	0.0	0.2768082801781201	0.0
31	0.0	0.0	0.0	0.5977454166165203	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAGAG	35	2.3431247E-5	31.714287	5
CTTGTTC	25	0.005446675	29.599998	3
TATACAC	45	3.908106E-6	28.777777	3
TTATACA	50	8.865514E-6	25.899998	2
CCGTCTT	45	1.2985778E-4	24.666666	37
GCCGTCT	45	1.2985778E-4	24.666666	36
ATACACA	45	1.2985778E-4	24.666666	4
GGAGAGA	45	0.0037754646	20.555555	6
TGCCGTC	55	5.0488824E-4	20.181818	35
ATGCCGT	60	9.068987E-4	18.5	34
TCCTGTC	50	0.0069431947	18.499998	35
CACATCT	50	0.0069431947	18.499998	7
TACACAT	50	0.0069431947	18.499998	5
TATGCCG	65	0.001551461	17.076921	33
CTTATAC	85	5.237479E-4	15.235294	1
CGTATGC	75	0.004031643	14.8	31
TCTTATA	75	0.004031643	14.8	36
GTATGCC	75	0.004031643	14.8	32
TCGTATG	80	0.006187879	13.875001	30
CTCGTAT	85	0.009240743	13.058824	29
>>END_MODULE