FastQCFastQC Report
Fri 10 Feb 2017
ERR1632218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797074
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG17400.2182984264949051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.171251351819279No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC13090.16422565533438552No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC12860.1613401014209471No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC12090.15167976875421857No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG11740.14728870845116013No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT11730.14716324958535842No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC11110.13938479990565494No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG11080.13900842330824992No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG11040.13850658784504324No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA10830.1358719516632082No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA10060.12621161899647962No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT9710.12182055869342119No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG9530.11956229910899115No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC9360.11742949839036276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9260.11617490973234607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8900.11165839056348593No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA8780.11015288417386591No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA8760.10990196644226258No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC8700.10914921324745255No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG8620.10814554232103919No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG8540.10714187139462585No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT8400.10538544727340247No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC8360.10488361181019579No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC8300.10413085861538578No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA8290.10400539974958412No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG8030.1007434692387407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT250.005495344829.68
TACTATA250.005495344829.62
TATAGAG405.9378803E-527.755
GGTATCA6300.027.0158751
TATACTC358.867002E-426.428575
TCTATCG358.867002E-426.4285729
TTACACT551.9017105E-523.5454544
CGTGCGG400.001930670723.12510
CTATCGC400.001930670723.12530
TAACCTA450.003824795620.5555535
TACATTG500.007033035618.55
ATATACA500.007033035618.54
TAACACT609.233427E-418.54
ATAACAC500.007033035618.53
GAACATA500.007033035618.56
TATAACT500.007033035618.54
TATCGCC500.007033035618.531
TAGTCAG801.6156575E-518.55
ATACAGT609.233427E-418.56
CTTATAC7800.018.49999837
Produced by FastQC (version 0.11.5)