##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632218.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797074
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.754328958164486	34.0	31.0	34.0	31.0	34.0
2	32.91304195093554	34.0	31.0	34.0	31.0	34.0
3	32.92792388159694	34.0	33.0	34.0	31.0	34.0
4	36.378503125180345	37.0	37.0	37.0	35.0	37.0
5	36.29197163625962	37.0	37.0	37.0	35.0	37.0
6	36.32091625118872	37.0	37.0	37.0	35.0	37.0
7	36.296855749905276	37.0	37.0	37.0	35.0	37.0
8	36.279247598090016	37.0	37.0	37.0	35.0	37.0
9	38.090122121659974	39.0	39.0	39.0	37.0	39.0
10	38.010494634124306	39.0	38.0	39.0	35.0	39.0
11	38.12451792430816	39.0	38.0	39.0	37.0	39.0
12	38.05085600584137	39.0	38.0	39.0	35.0	39.0
13	38.09365127955497	39.0	38.0	39.0	37.0	39.0
14	39.49595646075521	40.0	39.0	41.0	37.0	41.0
15	39.51798452841267	40.0	39.0	41.0	37.0	41.0
16	39.41714696502458	40.0	39.0	41.0	37.0	41.0
17	39.444148222122415	40.0	39.0	41.0	37.0	41.0
18	39.47029761352145	41.0	39.0	41.0	37.0	41.0
19	39.50156949041118	41.0	39.0	41.0	37.0	41.0
20	39.49765015544354	41.0	39.0	41.0	37.0	41.0
21	39.47646768054158	41.0	39.0	41.0	37.0	41.0
22	39.455938846330454	41.0	39.0	41.0	37.0	41.0
23	39.412316798691215	41.0	39.0	41.0	37.0	41.0
24	39.355993295478214	41.0	39.0	41.0	36.0	41.0
25	39.29690844262892	40.0	39.0	41.0	36.0	41.0
26	39.210133563508535	40.0	39.0	41.0	36.0	41.0
27	39.131568712566214	40.0	39.0	41.0	36.0	41.0
28	39.069903170847375	40.0	39.0	41.0	36.0	41.0
29	38.91852073960511	40.0	39.0	41.0	35.0	41.0
30	38.877809337652465	40.0	38.0	41.0	35.0	41.0
31	38.779277708217805	40.0	38.0	41.0	35.0	41.0
32	38.69670570110178	40.0	38.0	41.0	35.0	41.0
33	38.564068078998936	40.0	38.0	41.0	35.0	41.0
34	38.52154379643546	40.0	38.0	41.0	35.0	41.0
35	38.49853464044744	40.0	38.0	41.0	35.0	41.0
36	38.453811063966455	40.0	38.0	41.0	34.0	41.0
37	38.40122623495434	40.0	38.0	41.0	34.0	41.0
38	38.349651349811936	40.0	38.0	41.0	34.0	41.0
39	38.11740942497183	40.0	37.0	41.0	34.0	41.0
40	38.102536276431046	40.0	37.0	41.0	34.0	41.0
41	38.08953748334534	40.0	37.0	41.0	34.0	41.0
42	37.93351432865706	40.0	37.0	41.0	34.0	41.0
43	37.05718540562106	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	0.0
13	0.0
14	1.0
15	2.0
16	2.0
17	2.0
18	14.0
19	20.0
20	42.0
21	102.0
22	171.0
23	282.0
24	492.0
25	753.0
26	1135.0
27	1623.0
28	2506.0
29	3507.0
30	4941.0
31	6818.0
32	9485.0
33	13505.0
34	20038.0
35	30104.0
36	49561.0
37	94335.0
38	196840.0
39	360787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.903609953404576	17.091637664758856	11.332573888999013	26.672178492837556
2	19.436714784323662	19.77846473476741	33.08701576014273	27.6978047207662
3	20.634470576132202	20.096377500708844	28.70310661243498	30.566045310723972
4	14.189397722168835	14.931988748848916	33.295528395105094	37.583085133877155
5	16.795429282601113	34.0849155787292	32.50563937601779	16.6140157626519
6	37.18098946898281	32.81778103413234	15.327435093855776	14.673794403029078
7	32.45909413680537	28.599477589282802	18.980546348268792	19.96088192564304
8	29.704268361532304	31.08419042648487	19.070249437316985	20.14129177466584
9	28.164135324951005	12.428833458374003	17.649302323247277	41.75772889342771
10	17.536891179488983	25.241069210637907	31.50886868722352	25.713170922649592
11	39.16273771318598	19.907310989945724	19.19809202156889	21.73185927529941
12	23.63005191487867	23.39582021242695	26.933509310302433	26.04061856239195
13	33.248230402697864	17.29626107488138	22.690365010024163	26.765143512396588
14	24.852397644384336	18.503802658222448	23.208886502382462	33.43491319501075
15	26.670045692118926	25.428002920682395	20.751774615656764	27.15017677154191
16	28.356714683956568	23.17990550438228	21.89821773135242	26.56516208030873
17	26.17473408993393	24.2636191871771	22.794997704102755	26.76664901878621
18	26.036854796417895	22.280239977718505	24.438383387238826	27.24452183862477
19	28.145567412812362	23.526799268323895	23.38515620883381	24.942477110029934
20	28.274539126856478	22.595769025209705	23.06272692372352	26.0669649242103
21	27.576360538670187	23.12369993250313	22.341965739692927	26.957973789133753
22	28.707999508201247	22.50079666379784	22.46177895653352	26.329424871467396
23	25.97926917701493	22.90427237621601	23.027096605835844	28.089361840933215
24	26.599788727269992	23.365584625768747	22.913054496822127	27.121572150139134
25	26.859363120613644	22.40456971372796	23.356927964028433	27.37913920162996
26	27.228337644936357	22.850701440518698	22.235952998090518	27.68500791645443
27	27.347272649716338	22.811432815522775	22.34296941061934	27.498325124141548
28	26.647337637408825	23.271992311880705	23.208510125785057	26.872159924925416
29	26.81407247005924	23.61148400274002	23.6326865510605	25.941756976140233
30	26.937147617410677	22.788724760812674	22.63152480196318	27.642602819813465
31	25.940502387482216	22.782702735254194	23.1363712779491	28.14042359931449
32	25.340432632352833	23.498445564652716	23.548754569839186	27.612367233155265
33	26.013393988512984	22.550478374655302	24.08019832537506	27.35592931145665
34	27.166110047498726	22.395034839927032	24.275286861696653	26.163568250877585
35	26.879687456873512	22.415861011650108	23.702818057043636	27.00163347443274
36	25.504532828821414	22.448354857892745	23.40184223798543	28.64527007530041
37	25.080356403545967	21.935353555629717	25.946524413040695	27.037765627783617
38	24.967192506592863	21.51556819065733	24.97296361441974	28.544275688330067
39	24.598594358867558	22.184138486514428	26.76451621806758	26.452750936550434
40	25.60715818104718	20.9798588336842	26.50745100203996	26.90553198322866
41	23.709969212394334	21.43665456406808	27.695421002315975	27.15795522122162
42	23.080165706069952	21.90047599093685	27.950855253088168	27.06850304990503
43	23.029354865420274	20.318941528641005	28.004677106517086	28.647026499421635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	18.0
2	21.0
3	69.5
4	118.0
5	118.0
6	167.5
7	217.0
8	224.5
9	232.0
10	328.0
11	424.0
12	424.0
13	738.5
14	1053.0
15	1780.5
16	2508.0
17	2398.0
18	2288.0
19	2288.0
20	2576.5
21	2865.0
22	2978.5
23	3092.0
24	3884.5
25	4677.0
26	4677.0
27	5502.0
28	6327.0
29	7751.0
30	9175.0
31	10966.0
32	12757.0
33	12757.0
34	16245.5
35	19734.0
36	22162.0
37	24590.0
38	26638.5
39	28687.0
40	28687.0
41	30994.0
42	33301.0
43	37551.0
44	41801.0
45	43029.5
46	44258.0
47	44258.0
48	46487.5
49	48717.0
50	51838.5
51	54960.0
52	60192.5
53	65425.0
54	65425.0
55	65930.0
56	66435.0
57	62372.0
58	58309.0
59	59532.5
60	60756.0
61	60756.0
62	57418.0
63	54080.0
64	45770.5
65	37461.0
66	34906.5
67	32352.0
68	32352.0
69	28729.0
70	25106.0
71	25281.5
72	25457.0
73	21230.0
74	17003.0
75	17003.0
76	11013.0
77	5023.0
78	4112.5
79	3202.0
80	2634.0
81	2066.0
82	2066.0
83	1645.0
84	1224.0
85	944.5
86	665.0
87	537.0
88	409.0
89	409.0
90	318.0
91	227.0
92	135.5
93	44.0
94	28.0
95	12.0
96	12.0
97	6.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	797074.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.24463422247474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53438170670682	53.33721442033511
2	6.701320141287435	8.074371614391286
3	1.7010696125118994	3.0744094977823866
4	0.7967870153163529	1.9200856916380422
5	0.45995260964598855	1.3854838363897644
6	0.31670926290890117	1.14480202192898
7	0.21005158580467528	0.88581366682574
8	0.15652235085063398	0.7543705419710641
9	0.123982398765311	0.6722346837277048
>10	0.8239183153299545	9.445326695702924
>50	0.08713053306806649	3.700955853809974
>100	0.07501098090597012	9.256541885617604
>500	0.010656156060248634	4.502015618385588
>1k	0.002507330837705561	1.8463739714939131
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1740	0.2182984264949051	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1365	0.171251351819279	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1309	0.16422565533438552	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	1286	0.1613401014209471	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	1209	0.15167976875421857	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	1174	0.14728870845116013	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1173	0.14716324958535842	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	1111	0.13938479990565494	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1108	0.13900842330824992	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	1104	0.13850658784504324	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1083	0.1358719516632082	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	1006	0.12621161899647962	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	971	0.12182055869342119	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	953	0.11956229910899115	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	936	0.11742949839036276	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.11617490973234607	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	890	0.11165839056348593	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	878	0.11015288417386591	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	876	0.10990196644226258	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	870	0.10914921324745255	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	862	0.10814554232103919	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	854	0.10714187139462585	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	840	0.10538544727340247	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	836	0.10488361181019579	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	830	0.10413085861538578	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	829	0.10400539974958412	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	803	0.1007434692387407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.254588658016696E-4	0.0
2	0.0	0.0	0.0	3.763765974050088E-4	0.0
3	0.0	0.0	0.0	5.018354632066784E-4	0.0
4	0.0	0.0	0.0	5.018354632066784E-4	0.0
5	0.0	0.0	0.0	6.27294329008348E-4	0.0
6	0.0	0.0	0.0	6.27294329008348E-4	0.0
7	0.0	0.0	0.0	6.27294329008348E-4	0.0
8	0.0	0.0	0.0	7.527531948100176E-4	0.0
9	0.0	0.0	0.0	0.0010036709264133567	0.0
10	0.0	0.0	0.0	0.001254588658016696	0.0
11	0.0	0.0	0.0	0.0013800475238183658	0.0
12	0.0	0.0	0.0	0.001630965255421705	0.0
13	0.0	0.0	0.0	0.001630965255421705	0.0
14	0.0	0.0	0.0	0.001630965255421705	0.0
15	0.0	0.0	0.0	0.0017564241212233746	0.0
16	0.0	0.0	0.0	0.002258259584430053	0.0
17	0.0	0.0	0.0	0.0027600950476367315	0.0
18	0.0	0.0	0.0	0.0033873893766450795	0.0
19	0.0	0.0	0.0	0.0038892248398517577	0.0
20	0.0	0.0	0.0	0.004641978034661776	0.0
21	0.0	0.0	0.0	0.005018354632066784	0.0
22	0.0	0.0	0.0	0.006022025558480141	0.0
23	0.0	0.0	0.0	0.007402073082298507	0.0
24	0.0	0.0	0.0	0.00953487380092689	0.0
25	0.0	0.0	0.0	0.010664003593141916	0.0
26	0.0	0.0	0.0	0.014176851835588665	0.0
27	0.0	0.0	0.0	0.032744763974235765	0.0
28	0.0	0.0	0.0	0.10827100118684087	0.0
29	0.0	0.0	0.0	0.242261069863024	0.0
30	0.0	0.0	0.0	0.4072394783922195	0.0
31	0.0	0.0	0.0	0.8872450989494075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGT	25	0.0054953448	29.6	8
TACTATA	25	0.0054953448	29.6	2
TATAGAG	40	5.9378803E-5	27.75	5
GGTATCA	630	0.0	27.015875	1
TATACTC	35	8.867002E-4	26.42857	5
TCTATCG	35	8.867002E-4	26.42857	29
TTACACT	55	1.9017105E-5	23.545454	4
CGTGCGG	40	0.0019306707	23.125	10
CTATCGC	40	0.0019306707	23.125	30
TAACCTA	45	0.0038247956	20.555553	5
TACATTG	50	0.0070330356	18.5	5
ATATACA	50	0.0070330356	18.5	4
TAACACT	60	9.233427E-4	18.5	4
ATAACAC	50	0.0070330356	18.5	3
GAACATA	50	0.0070330356	18.5	6
TATAACT	50	0.0070330356	18.5	4
TATCGCC	50	0.0070330356	18.5	31
TAGTCAG	80	1.6156575E-5	18.5	5
ATACAGT	60	9.233427E-4	18.5	6
CTTATAC	780	0.0	18.499998	37
>>END_MODULE