FastQCFastQC Report
Fri 10 Feb 2017
ERR1632217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826343
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT250.00549546129.5999984
TACACTT250.00549546129.5999985
GGTATCA5050.025.277231
CTATATA400.001930730323.1252
TATGCCG753.7381324E-722.233
GCCGTCT909.4702045E-820.55555736
TATATAC555.141412E-420.1818183
GTATATC555.141412E-420.1818183
GGCTTAT609.233819E-418.51
TCGTATG902.1507258E-618.530
ATGCGTT801.6157743E-518.536
ATGCCGT902.1507258E-618.534
CGTATGC953.6039055E-617.52631631
TGCGTTA953.6039055E-617.52631637
AAATGCG852.7216156E-517.41176434
TACTAGA752.0663807E-417.2666662
GTGCTAG1801.0913936E-1116.4444451
CGTTTAG904.4435226E-516.44444526
GTTCTAG1606.2755134E-1016.18751
TTAGAGT803.3811262E-416.18754
Produced by FastQC (version 0.11.5)