##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632217.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	826343
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79839969601001	34.0	31.0	34.0	31.0	34.0
2	32.95884517688175	34.0	33.0	34.0	31.0	34.0
3	32.95762413428806	34.0	33.0	34.0	31.0	34.0
4	36.39408937934974	37.0	37.0	37.0	35.0	37.0
5	36.311681710863404	37.0	37.0	37.0	35.0	37.0
6	36.34940696538847	37.0	37.0	37.0	35.0	37.0
7	36.34095405902875	37.0	37.0	37.0	35.0	37.0
8	36.32453714740731	37.0	37.0	37.0	35.0	37.0
9	38.106511460737245	39.0	39.0	39.0	37.0	39.0
10	38.04990421652994	39.0	38.0	39.0	37.0	39.0
11	38.14767838536782	39.0	39.0	39.0	37.0	39.0
12	38.124808947374156	39.0	38.0	39.0	37.0	39.0
13	38.139952779898906	39.0	39.0	39.0	37.0	39.0
14	39.56689534491125	41.0	39.0	41.0	37.0	41.0
15	39.597375424006735	41.0	39.0	41.0	37.0	41.0
16	39.47756319107199	41.0	39.0	41.0	37.0	41.0
17	39.533214415805546	41.0	39.0	41.0	37.0	41.0
18	39.54321510559175	41.0	39.0	41.0	37.0	41.0
19	39.59686958079151	41.0	39.0	41.0	37.0	41.0
20	39.579122712965436	41.0	39.0	41.0	37.0	41.0
21	39.54936267385335	41.0	39.0	41.0	37.0	41.0
22	39.53735676347473	41.0	39.0	41.0	37.0	41.0
23	39.50215104381594	41.0	39.0	41.0	37.0	41.0
24	39.45473852867393	41.0	39.0	41.0	37.0	41.0
25	39.399543530955064	41.0	39.0	41.0	37.0	41.0
26	39.32277516721265	40.0	39.0	41.0	36.0	41.0
27	39.25858390523064	40.0	39.0	41.0	36.0	41.0
28	39.19976208426767	40.0	39.0	41.0	36.0	41.0
29	39.07418590101205	40.0	39.0	41.0	36.0	41.0
30	39.03709718603534	40.0	39.0	41.0	35.0	41.0
31	38.97498254356847	40.0	39.0	41.0	35.0	41.0
32	38.92348818831889	40.0	38.0	41.0	35.0	41.0
33	38.788442571668185	40.0	38.0	41.0	35.0	41.0
34	38.80037829327531	40.0	38.0	41.0	35.0	41.0
35	38.76789904434357	40.0	38.0	41.0	35.0	41.0
36	38.743491504133274	40.0	38.0	41.0	35.0	41.0
37	38.711810955015046	40.0	38.0	41.0	35.0	41.0
38	38.641439450688154	40.0	38.0	41.0	35.0	41.0
39	38.46106761962042	40.0	38.0	41.0	35.0	41.0
40	38.46518939471866	40.0	38.0	41.0	35.0	41.0
41	38.47425705790452	40.0	38.0	41.0	35.0	41.0
42	38.342763235121495	40.0	38.0	41.0	34.0	41.0
43	37.535001809176094	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	7.0
18	13.0
19	28.0
20	43.0
21	109.0
22	192.0
23	282.0
24	486.0
25	706.0
26	1074.0
27	1670.0
28	2364.0
29	3369.0
30	4599.0
31	6428.0
32	8923.0
33	12540.0
34	18336.0
35	27477.0
36	44493.0
37	82167.0
38	196533.0
39	414502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96242601437902	18.218463761416263	12.23511302207437	26.583997202130348
2	19.994965770872387	20.35510677769401	32.79558246394052	26.85434498749309
3	20.938036626437206	21.562474662458566	29.172389673537502	28.32709903756672
4	15.933940264514856	15.349316204046021	32.71426030110983	36.0024832303293
5	16.407593456954316	34.593867195583435	32.92603676681475	16.072502580647505
6	36.06758936664315	32.96839205995573	15.180258076851864	15.783760496549254
7	30.063424026100545	28.947422559397246	20.498751729003573	20.490401685498636
8	27.756028670903003	31.69168250956322	19.69145984173642	20.860828977797354
9	26.85035148842551	14.151750544265518	19.155846906187865	39.84205106112111
10	18.115237861275524	25.9359612170733	32.13435583044813	23.814445091203048
11	37.01913128083616	20.79136629704614	20.466803736462946	21.722698685654745
12	22.75459464169237	24.56933743009864	27.2429245482808	25.43314337992819
13	31.540050560118498	18.876180956334114	23.38399429776739	26.199774185779994
14	24.000929396146635	20.224410444573255	23.15890616850388	32.61575399077623
15	26.762978569431823	26.765398871897023	20.813149019232934	25.658473539438226
16	26.605900939440403	25.01092161487421	22.54802182628763	25.835155619397753
17	25.42999698672343	25.30051080483528	23.636915905380697	25.632576303060596
18	24.888696340381657	24.141185924004922	25.06271608762947	25.907401647983946
19	26.1438651988339	24.367363189377777	24.825405430916703	24.663366180871623
20	26.356851815771414	23.7330019132491	24.53702639218823	25.37311987879125
21	26.19844301942414	24.290881631477486	23.89141070959638	25.619264639502
22	26.504611281271824	23.88971649787074	23.795566731974496	25.810105488882947
23	25.731687689010496	24.079831256512126	23.954943649307854	26.23353740516952
24	26.225308376787847	24.378254550471173	23.955064664431113	25.441372408309864
25	26.291019588718	23.759504225243028	23.96946546409905	25.980010721939923
26	26.003487655852354	24.25227780715756	23.86309317075355	25.881141366236537
27	25.929789445787037	23.89213680033594	23.99633082146276	26.181742932414267
28	25.45359493575912	24.058048534325334	24.49079861510293	25.997557914812614
29	25.38195398278923	24.48644207066557	24.49551820491007	25.636085741635135
30	25.185183392368543	24.86582448208553	24.291486707093785	25.657505418452143
31	25.85500209961239	24.233641478175528	24.258207548197298	25.653148874014786
32	24.971591699814727	24.06579350221397	24.441787490182648	26.520827307788654
33	25.009711463641608	24.006980152309634	24.62028479699108	26.363023587057675
34	25.651696692535666	23.634374587792237	24.64763421484783	26.066294504824267
35	25.488447291257987	23.662813141758328	24.88845431013514	25.960285256848547
36	24.96239455044697	24.10246108456174	24.947025629792954	25.98811873519834
37	25.652059737905446	22.76076641297863	25.381106876926406	26.20606697218951
38	24.815361175686128	23.140148824398583	26.17605522162105	25.86843477829424
39	24.699307672479833	22.781944059549122	26.6702809850147	25.84846728295635
40	24.17797452147595	22.49610633840911	27.356436733898637	25.969482406216304
41	23.528728385186295	22.771052698455726	27.673496356839717	26.026722559518262
42	23.867691745437426	22.541244979385073	27.594110435981182	25.996952839196314
43	23.09525221366914	22.487151219287874	27.859254571043746	26.55834199599924
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	24.0
2	28.0
3	86.0
4	144.0
5	144.0
6	158.5
7	173.0
8	167.0
9	161.0
10	262.0
11	363.0
12	363.0
13	633.5
14	904.0
15	1391.0
16	1878.0
17	2003.5
18	2129.0
19	2129.0
20	2754.5
21	3380.0
22	3868.0
23	4356.0
24	5441.0
25	6526.0
26	6526.0
27	7562.5
28	8599.0
29	11546.5
30	14494.0
31	16277.0
32	18060.0
33	18060.0
34	20905.0
35	23750.0
36	26733.0
37	29716.0
38	34030.5
39	38345.0
40	38345.0
41	42227.5
42	46110.0
43	46155.0
44	46200.0
45	49588.5
46	52977.0
47	52977.0
48	55830.5
49	58684.0
50	61285.0
51	63886.0
52	66840.0
53	69794.0
54	69794.0
55	64312.0
56	58830.0
57	57039.5
58	55249.0
59	52525.5
60	49802.0
61	49802.0
62	46733.5
63	43665.0
64	40377.0
65	37089.0
66	33013.0
67	28937.0
68	28937.0
69	25363.5
70	21790.0
71	18798.5
72	15807.0
73	12532.5
74	9258.0
75	9258.0
76	7507.5
77	5757.0
78	4854.5
79	3952.0
80	3191.5
81	2431.0
82	2431.0
83	1928.0
84	1425.0
85	1141.0
86	857.0
87	655.0
88	453.0
89	453.0
90	364.5
91	276.0
92	171.5
93	67.0
94	40.5
95	14.0
96	14.0
97	9.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	826343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.1020472146796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.89702346776097	56.095841721071324
2	6.281596091386173	7.927631462843943
3	1.6845688558070104	3.1889923042653834
4	0.7902862500113539	1.9947472104531416
5	0.4591464533315519	1.4486540588290138
6	0.3079707501165343	1.1660150888756307
7	0.21343522453802438	0.9427739731251908
8	0.1584868617983077	0.800067634888257
9	0.12430551097398371	0.7059539000272673
>10	0.8820988813124302	11.29671362483749
>50	0.12242381774670563	5.383399515377821
>100	0.07672994710189858	8.292280265738125
>500	0.0019278881151044217	0.7569292396674276
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.4203024651990758E-4	0.0
13	0.0	0.0	0.0	3.630453697798614E-4	0.0
14	0.0	0.0	0.0	3.630453697798614E-4	0.0
15	0.0	0.0	0.0	7.260907395597228E-4	0.0
16	0.0	0.0	0.0	0.0013311663558594918	0.0
17	1.2101512325995379E-4	0.0	0.0	0.0020572570954192145	0.0
18	1.2101512325995379E-4	0.0	0.0	0.0021782722186791683	0.0
19	1.2101512325995379E-4	0.0	0.0	0.002420302465199076	0.0
20	1.2101512325995379E-4	0.0	0.0	0.0027833478349789374	0.0
21	1.2101512325995379E-4	0.0	0.0	0.003025378081498845	0.0
22	1.2101512325995379E-4	0.0	0.0	0.0033884234512787065	0.0
23	2.4203024651990758E-4	0.0	0.0	0.004840604930398152	0.0
24	2.4203024651990758E-4	0.0	0.0	0.007623952765377089	0.0
25	2.4203024651990758E-4	0.0	0.0	0.009318164491016443	0.0
26	2.4203024651990758E-4	0.0	0.0	0.013795724051634733	0.0
27	2.4203024651990758E-4	0.0	0.0	0.029769720321948634	0.0
28	2.4203024651990758E-4	0.0	0.0	0.0780547545026702	0.0
29	2.4203024651990758E-4	0.0	0.0	0.1458232235282443	0.0
30	2.4203024651990758E-4	0.0	0.0	0.2310178703032518	0.0
31	2.4203024651990758E-4	0.0	0.0	0.46082558937390405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	25	0.005495461	29.599998	4
TACACTT	25	0.005495461	29.599998	5
GGTATCA	505	0.0	25.27723	1
CTATATA	40	0.0019307303	23.125	2
TATGCCG	75	3.7381324E-7	22.2	33
GCCGTCT	90	9.4702045E-8	20.555557	36
TATATAC	55	5.141412E-4	20.181818	3
GTATATC	55	5.141412E-4	20.181818	3
GGCTTAT	60	9.233819E-4	18.5	1
TCGTATG	90	2.1507258E-6	18.5	30
ATGCGTT	80	1.6157743E-5	18.5	36
ATGCCGT	90	2.1507258E-6	18.5	34
CGTATGC	95	3.6039055E-6	17.526316	31
TGCGTTA	95	3.6039055E-6	17.526316	37
AAATGCG	85	2.7216156E-5	17.411764	34
TACTAGA	75	2.0663807E-4	17.266666	2
GTGCTAG	180	1.0913936E-11	16.444445	1
CGTTTAG	90	4.4435226E-5	16.444445	26
GTTCTAG	160	6.2755134E-10	16.1875	1
TTAGAGT	80	3.3811262E-4	16.1875	4
>>END_MODULE