FastQCFastQC Report
Fri 10 Feb 2017
ERR1632216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534260
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5520.10332048066484484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT250.00549374329.66
GGTATCA3600.020.5555551
CGTCGTA450.003823168620.55555510
TATACAA555.1381445E-420.1818182
GTATTAG500.007030074518.51
CGAGCGA752.0646576E-417.26666610
CTCTATG1301.3880708E-817.0769231
CTTATAC4200.015.85714437
GTACAAT700.002590058815.8571431
GGGCTAC700.002590058815.8571431
GTGTACC700.002590058815.8571431
GATACCG750.004100582614.85
TTATACT750.004100582614.84
CCTATAC750.004100582614.83
GCCGTCT1001.0916247E-414.836
CTCGTAT1152.2053602E-514.47826129
GCGACCA908.2638004E-414.38888813
CATACAC908.2638004E-414.3888883
CTCTTAT9800.014.15816437
TATGGGC1702.426168E-814.14705854
Produced by FastQC (version 0.11.5)