##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632216.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	534260
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72780668588328	34.0	31.0	34.0	31.0	34.0
2	32.885220679070116	34.0	31.0	34.0	31.0	34.0
3	32.89193838206117	34.0	31.0	34.0	31.0	34.0
4	36.346657058361096	37.0	37.0	37.0	35.0	37.0
5	36.25979485643694	37.0	37.0	37.0	35.0	37.0
6	36.29253359787369	37.0	37.0	37.0	35.0	37.0
7	36.28275933066298	37.0	37.0	37.0	35.0	37.0
8	36.26141017482125	37.0	37.0	37.0	35.0	37.0
9	38.03824168008086	39.0	38.0	39.0	35.0	39.0
10	37.968444203196945	39.0	38.0	39.0	35.0	39.0
11	38.07149328042526	39.0	38.0	39.0	37.0	39.0
12	38.03859356867443	39.0	38.0	39.0	35.0	39.0
13	38.060762550069256	39.0	38.0	39.0	35.0	39.0
14	39.46755325122599	40.0	39.0	41.0	37.0	41.0
15	39.490272526485235	40.0	39.0	41.0	37.0	41.0
16	39.369194774080036	40.0	39.0	41.0	37.0	41.0
17	39.41474001422528	40.0	39.0	41.0	37.0	41.0
18	39.42985999326171	40.0	39.0	41.0	37.0	41.0
19	39.47987871074009	41.0	39.0	41.0	37.0	41.0
20	39.44808707370943	40.0	39.0	41.0	37.0	41.0
21	39.427799198891925	40.0	39.0	41.0	37.0	41.0
22	39.407874443155016	40.0	39.0	41.0	37.0	41.0
23	39.36848912514506	40.0	39.0	41.0	36.0	41.0
24	39.32120877475386	40.0	39.0	41.0	36.0	41.0
25	39.25626099651855	40.0	39.0	41.0	36.0	41.0
26	39.1705723804889	40.0	39.0	41.0	36.0	41.0
27	39.09734773331337	40.0	39.0	41.0	36.0	41.0
28	39.035001684573054	40.0	39.0	41.0	35.0	41.0
29	38.89660464942163	40.0	39.0	41.0	35.0	41.0
30	38.86199041665107	40.0	38.0	41.0	35.0	41.0
31	38.78931793508779	40.0	38.0	41.0	35.0	41.0
32	38.729642870512485	40.0	38.0	41.0	35.0	41.0
33	38.59477969527945	40.0	38.0	41.0	35.0	41.0
34	38.58151461835062	40.0	38.0	41.0	35.0	41.0
35	38.559100437988995	40.0	38.0	41.0	35.0	41.0
36	38.537178527308804	40.0	38.0	41.0	35.0	41.0
37	38.49717553251226	40.0	38.0	41.0	35.0	41.0
38	38.42644405345712	40.0	38.0	41.0	34.0	41.0
39	38.23327406131846	40.0	38.0	41.0	34.0	41.0
40	38.23887807434583	40.0	38.0	41.0	34.0	41.0
41	38.237060607195	40.0	38.0	41.0	34.0	41.0
42	38.1091154119717	40.0	38.0	41.0	34.0	41.0
43	37.25621233107476	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	8.0
19	11.0
20	36.0
21	78.0
22	104.0
23	200.0
24	342.0
25	559.0
26	863.0
27	1200.0
28	1714.0
29	2407.0
30	3532.0
31	4861.0
32	6641.0
33	9163.0
34	13252.0
35	20074.0
36	31643.0
37	57441.0
38	134241.0
39	245886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4726537640849	17.618762400329427	11.87324523640175	27.035338599183916
2	20.46119866731554	19.52495039868229	32.61164975854453	27.40220117545764
3	21.234979223598994	20.761988544903232	28.316737169168572	29.686295062329204
4	16.063527121626176	15.035188859356868	32.009134129450075	36.89214988956688
5	17.198742185452776	33.61266050237712	32.40912664245873	16.779470669711376
6	37.129674690225734	32.010631527720584	14.85250627035526	16.007187511698422
7	31.260247819413767	27.888668438587956	19.91783026990604	20.93325347209224
8	28.37981507131359	31.377232059296972	19.039980533822483	21.20297233556695
9	27.311608580092088	13.483322726762252	18.90970688428855	40.29536180885711
10	18.458802830082732	25.259424250364994	31.7070340283757	24.57473889117658
11	37.867143338449445	20.22161494403474	19.821996780593718	22.089244936922096
12	23.06442556058848	23.87152322839067	27.496162916931834	25.56788829408902
13	32.62512634297907	18.378692022610714	22.160745704338712	26.8354359300715
14	24.354995694979973	19.501927900273273	23.141541571519486	33.00153483322727
15	27.409126642458727	25.535132706921726	20.532137910380712	26.523602740238832
16	26.97993486317523	24.05551604087897	22.425036499082843	26.539512596862952
17	25.421517613147156	24.847452551192305	23.702130049039795	26.028899786620745
18	25.76722943885	23.65046980870737	24.397858720473177	26.18444203196945
19	26.326694867667428	23.993935537004454	24.17886422341182	25.500505371916294
20	26.559540298730955	23.52805750009359	24.258787856100025	25.653614345075432
21	26.978063115337104	23.374574177366824	23.628570359001237	26.018792348294838
22	27.111331561412044	23.491184067682404	23.47845618238311	25.919028188522443
23	26.61981057911878	23.50316325384644	23.519634634821998	26.35739153221278
24	26.38490622543331	23.81742971586868	23.31729869352001	26.480365365178006
25	26.5572942013252	23.13293153146408	23.652715906113126	26.65705836109759
26	26.386029274136185	23.5310522966346	23.6295062329203	26.45341219630891
27	26.15617863961367	23.57915621607457	23.778684535619362	26.485980608692394
28	26.041066147568596	23.38037659566503	24.031557668550892	26.546999588215474
29	25.82375622356156	23.758656833751356	24.259349380451464	26.158237562235616
30	25.907049002358402	23.896791824205444	23.945270093213043	26.25088908022311
31	26.32501029461311	23.463107850110433	23.72889604312507	26.482985812151387
32	25.741960843035226	23.58252536218321	24.01826825890016	26.657245535881408
33	25.581552053307377	23.391232733126195	24.429678433721406	26.597536779845022
34	26.140268782989555	23.047018305693857	24.109609553400965	26.70310335791562
35	25.610938494366035	23.188335267472766	24.859806086923967	26.340920151237224
36	25.58417250028076	23.13218283232883	24.48620521843297	26.797439448957437
37	25.345899000486654	22.406693370269156	25.283008273125446	26.964399356118747
38	24.861303485194476	22.38928611537454	26.107138846254635	26.642271553176357
39	24.69097443192453	22.1876988732078	26.817092801257814	26.30423389360985
40	24.49855875416464	21.745966383408827	27.212031595103507	26.543443267323024
41	23.772133418185902	22.034028375697225	27.616703477707482	26.57713472840939
42	23.60255306405121	22.04095384269831	27.80911915546738	26.547373937783103
43	23.146969640250063	21.76636843484446	28.11908059746191	26.96758132744357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	14.5
2	16.0
3	52.0
4	88.0
5	88.0
6	103.0
7	118.0
8	121.0
9	124.0
10	170.0
11	216.0
12	216.0
13	428.0
14	640.0
15	913.5
16	1187.0
17	1201.0
18	1215.0
19	1215.0
20	1613.0
21	2011.0
22	2131.0
23	2251.0
24	2795.0
25	3339.0
26	3339.0
27	4058.0
28	4777.0
29	5919.0
30	7061.0
31	8411.5
32	9762.0
33	9762.0
34	11644.0
35	13526.0
36	15412.0
37	17298.0
38	19529.5
39	21761.0
40	21761.0
41	23889.5
42	26018.0
43	27962.5
44	29907.0
45	32007.0
46	34107.0
47	34107.0
48	35738.0
49	37369.0
50	38570.5
51	39772.0
52	40849.5
53	41927.0
54	41927.0
55	41486.5
56	41046.0
57	40549.0
58	40052.0
59	38667.5
60	37283.0
61	37283.0
62	34660.0
63	32037.0
64	29174.0
65	26311.0
66	23391.0
67	20471.0
68	20471.0
69	17712.5
70	14954.0
71	12544.5
72	10135.0
73	8445.5
74	6756.0
75	6756.0
76	5546.0
77	4336.0
78	3559.5
79	2783.0
80	2266.5
81	1750.0
82	1750.0
83	1338.5
84	927.0
85	722.0
86	517.0
87	379.0
88	241.0
89	241.0
90	175.5
91	110.0
92	74.0
93	38.0
94	23.5
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	534260.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.58969320565508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.59004160140975	71.30443925338078
2	6.59169817486374	10.492624708833633
3	1.6899070557513645	4.034975523399689
4	0.6895989088609172	2.195398623647796
5	0.3623339296068066	1.4419023147702583
6	0.2336030351711768	1.115543634071029
7	0.1692284767287367	0.9428189781150346
8	0.11538656443829966	0.7346865010959056
9	0.0942412483649755	0.6750568840217709
>10	0.452179389025834	6.062783352896903
>50	0.00918966237052982	0.4904146066176134
>100	0.002356321279855388	0.40583415310746984
>500	2.356321279854398E-4	0.10352146604217756
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.10332048066484484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.871747838131247E-4	0.0
13	0.0	0.0	0.0	1.871747838131247E-4	0.0
14	0.0	0.0	0.0	1.871747838131247E-4	0.0
15	0.0	1.871747838131247E-4	0.0	5.615243514393741E-4	0.0
16	0.0	1.871747838131247E-4	0.0	5.615243514393741E-4	0.0
17	0.0	1.871747838131247E-4	0.0	0.001310223486691873	0.0
18	0.0	1.871747838131247E-4	0.0	0.0016845730543181222	0.0
19	0.0	1.871747838131247E-4	0.0	0.001871747838131247	0.0
20	0.0	1.871747838131247E-4	0.0	0.0022460974057574965	0.0
21	0.0	1.871747838131247E-4	0.0	0.003743495676262494	0.0
22	0.0	1.871747838131247E-4	0.0	0.003743495676262494	0.0
23	0.0	1.871747838131247E-4	0.0	0.005802418298206866	0.0
24	0.0	1.871747838131247E-4	0.0	0.008048515703964362	0.0
25	0.0	1.871747838131247E-4	0.0	0.010481787893534983	0.0
26	0.0	1.871747838131247E-4	0.0	0.014973982705049975	0.0
27	0.0	1.871747838131247E-4	0.0	0.03462733500542807	0.0
28	0.0	1.871747838131247E-4	0.0	0.09583348931231984	0.0
29	0.0	3.743495676262494E-4	0.0	0.16995470370231722	0.0
30	0.0	3.743495676262494E-4	0.0	0.2704675626099652	0.0
31	0.0	3.743495676262494E-4	0.0	0.5341968330026579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	25	0.005493743	29.6	6
GGTATCA	360	0.0	20.555555	1
CGTCGTA	45	0.0038231686	20.555555	10
TATACAA	55	5.1381445E-4	20.181818	2
GTATTAG	50	0.0070300745	18.5	1
CGAGCGA	75	2.0646576E-4	17.266666	10
CTCTATG	130	1.3880708E-8	17.076923	1
CTTATAC	420	0.0	15.857144	37
GTACAAT	70	0.0025900588	15.857143	1
GGGCTAC	70	0.0025900588	15.857143	1
GTGTACC	70	0.0025900588	15.857143	1
GATACCG	75	0.0041005826	14.8	5
TTATACT	75	0.0041005826	14.8	4
CCTATAC	75	0.0041005826	14.8	3
GCCGTCT	100	1.0916247E-4	14.8	36
CTCGTAT	115	2.2053602E-5	14.478261	29
GCGACCA	90	8.2638004E-4	14.388888	13
CATACAC	90	8.2638004E-4	14.388888	3
CTCTTAT	980	0.0	14.158164	37
TATGGGC	170	2.426168E-8	14.1470585	4
>>END_MODULE