FastQCFastQC Report
Fri 10 Feb 2017
ERR1632215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485925
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.10454288213201626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGC405.9331825E-527.756
GGTATCA1800.025.6944451
GCGTTAT451.3216767E-424.6666681
GTTATTC451.3216767E-424.6666683
ATAGTAC551.8997298E-523.5454543
CGTTATT400.001929592123.1252
ATGGTCG400.001929592123.12536
CCCTATG502.6989926E-422.22
TCTAATA502.6989926E-422.22
ATAGGGG603.7205893E-521.5833343
TCGTTTA450.00382267720.55555730
TGACCCG450.00382267720.55555713
GTGTTAG656.891657E-519.9230771
CTTAAGG500.007029181318.53
CCTTAGT500.007029181318.51
ACACCTC609.2263566E-418.56
GTCTAGA500.007029181318.51
GTTATGT609.2263566E-418.51
ACTGATC953.5977264E-617.5263168
TTATGCT953.5977264E-617.5263164
Produced by FastQC (version 0.11.5)