##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632215.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485925
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63389411946288	34.0	31.0	34.0	31.0	34.0
2	32.792266296239134	34.0	31.0	34.0	31.0	34.0
3	32.80743941966353	34.0	31.0	34.0	31.0	34.0
4	36.30255903688841	37.0	37.0	37.0	35.0	37.0
5	36.201642228739004	37.0	35.0	37.0	35.0	37.0
6	36.23226423830838	37.0	37.0	37.0	35.0	37.0
7	36.21982404692082	37.0	36.0	37.0	35.0	37.0
8	36.19907804702372	37.0	36.0	37.0	35.0	37.0
9	37.95972012141792	39.0	38.0	39.0	35.0	39.0
10	37.867862324432785	39.0	38.0	39.0	35.0	39.0
11	37.98248289345064	39.0	38.0	39.0	35.0	39.0
12	37.93998662345012	39.0	38.0	39.0	35.0	39.0
13	37.968799711889694	39.0	38.0	39.0	35.0	39.0
14	39.35095951021248	40.0	39.0	41.0	37.0	41.0
15	39.37851931882492	40.0	39.0	41.0	37.0	41.0
16	39.25764675618665	40.0	39.0	41.0	36.0	41.0
17	39.30430827802645	40.0	39.0	41.0	36.0	41.0
18	39.32567371507949	40.0	39.0	41.0	36.0	41.0
19	39.38138190049905	40.0	39.0	41.0	37.0	41.0
20	39.351395791531615	40.0	39.0	41.0	37.0	41.0
21	39.33604568606266	40.0	39.0	41.0	36.0	41.0
22	39.31061995163863	40.0	39.0	41.0	36.0	41.0
23	39.273136800946645	40.0	39.0	41.0	36.0	41.0
24	39.226936255595	40.0	39.0	41.0	36.0	41.0
25	39.16398003807172	40.0	39.0	41.0	36.0	41.0
26	39.08075320265473	40.0	39.0	41.0	36.0	41.0
27	39.0191202346041	40.0	39.0	41.0	36.0	41.0
28	38.96408705047075	40.0	39.0	41.0	35.0	41.0
29	38.82465401039255	40.0	38.0	41.0	35.0	41.0
30	38.78666872459742	40.0	38.0	41.0	35.0	41.0
31	38.71537377167258	40.0	38.0	41.0	35.0	41.0
32	38.66034058753923	40.0	38.0	41.0	35.0	41.0
33	38.52198178731285	40.0	38.0	41.0	35.0	41.0
34	38.52251273344652	40.0	38.0	41.0	35.0	41.0
35	38.49890209394454	40.0	38.0	41.0	34.0	41.0
36	38.486651232186034	40.0	38.0	41.0	34.0	41.0
37	38.458603693985694	40.0	38.0	41.0	34.0	41.0
38	38.39636569429439	40.0	38.0	41.0	34.0	41.0
39	38.22733137829912	40.0	38.0	41.0	34.0	41.0
40	38.237378196223695	40.0	38.0	41.0	34.0	41.0
41	38.24075526058548	40.0	38.0	41.0	34.0	41.0
42	38.123021042341925	40.0	38.0	41.0	34.0	41.0
43	37.27343725883624	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	3.0
18	8.0
19	17.0
20	25.0
21	68.0
22	101.0
23	220.0
24	318.0
25	493.0
26	771.0
27	1148.0
28	1679.0
29	2364.0
30	3321.0
31	4683.0
32	6184.0
33	8801.0
34	12628.0
35	18808.0
36	30073.0
37	54308.0
38	130910.0
39	208993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54108144260946	17.54674075217369	11.608581571230129	27.303596233986728
2	18.73149148531152	19.438184905077943	34.17914287184236	27.651180737768172
3	19.561249163965634	21.35350105468951	29.36955291454443	29.715696866800435
4	14.453876627051498	14.979677933837527	34.20301486854967	36.3634305705613
5	15.581622678396872	35.130524257858724	33.987343725883626	15.300509337860783
6	36.070792817821676	34.54998199310593	15.083603436744353	14.295621752328033
7	31.17806245819828	29.41667952873386	20.332355816226784	19.072902196841078
8	28.777280444513043	33.189278180789216	18.90312290991408	19.13031846478366
9	27.502186551422547	13.445284766167617	18.019653238668518	41.03287544374132
10	16.975459175798736	26.03961516694963	32.60359108915985	24.381334568091784
11	38.27792354787262	20.27226423830838	20.575603230951277	20.874208982867724
12	23.047178062458197	23.964192004939033	28.756495343931675	24.23213458867109
13	32.12532798271338	18.112054329371816	23.187940525801306	26.574677162113495
14	23.502392344497608	18.51643772187066	24.055975716417144	33.92519421721459
15	26.274836651746668	25.957297936924423	20.963728970520144	26.80413644080877
16	26.45716931625251	24.734063898749806	22.625096465503937	26.18367031949375
17	25.008797653958943	24.99891958635592	24.194268662859493	25.798014096825643
18	25.501672068734887	24.16607501157586	24.779338375263674	25.55291454442558
19	26.288213201625766	23.95451973041107	24.377630292740648	25.379636775222515
20	26.542367649328604	23.98353655399496	24.157637495498278	25.316458301178162
21	26.653084323712505	23.930236147553636	23.472140762463344	25.944538766270515
22	26.734784174512527	23.82034264546998	23.534290271132377	25.910582908885115
23	26.210423419251942	23.844214642177292	23.99032772547204	25.955034213098727
24	26.076657920460978	23.936204146730464	23.521736893553534	26.465401039255028
25	26.242938725111898	23.65920666769563	24.2014714204867	25.89638318670577
26	25.901733806657408	23.87281987961105	24.089725780727477	26.135720533004065
27	25.777023203169215	23.905952564696197	24.07490867932294	26.242115552811647
28	25.951535730822656	23.806348716365694	24.12182950043731	26.120286052374336
29	25.744507897309255	24.10125019293101	24.41282090857643	25.741421001183312
30	25.645727221279003	24.30128106189227	23.940937387456913	26.112054329371816
31	26.043319442300767	24.026341513608067	23.943201111282605	25.98713793280856
32	25.287029891444153	24.177393630704326	24.360549467510417	26.1750270103411
33	25.373462982970622	23.8909296702166	24.712867212018317	26.022740134794464
34	26.031589237022175	23.199670731079898	24.522714410660083	26.246025621237845
35	25.461954005247723	23.691927766630652	24.905283737202243	25.94083449091938
36	25.61671039769512	23.653032875443742	24.429490147656534	26.30076657920461
37	25.36235015691722	22.91608787364305	25.011267170859703	26.710294798580026
38	24.941503318413336	23.17723928589803	25.423264907135874	26.45799248855276
39	24.848279055409787	22.74857231054175	26.078510058136544	26.32463857591192
40	24.623758810516026	22.250553068889232	26.905180840664713	26.22050727993003
41	23.721561969439726	22.238411277460514	27.496630138395844	26.54339661470392
42	23.61002212275557	22.290271132376397	27.671760045274475	26.42794669959356
43	23.153573082265783	21.633585429850285	28.11092246745897	27.101919020424965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	5.0
2	9.0
3	28.0
4	47.0
5	47.0
6	62.5
7	78.0
8	76.5
9	75.0
10	118.0
11	161.0
12	161.0
13	288.0
14	415.0
15	713.5
16	1012.0
17	1018.0
18	1024.0
19	1024.0
20	1326.0
21	1628.0
22	1811.0
23	1994.0
24	2489.0
25	2984.0
26	2984.0
27	3743.5
28	4503.0
29	5590.5
30	6678.0
31	8059.5
32	9441.0
33	9441.0
34	11182.0
35	12923.0
36	14942.0
37	16961.0
38	19134.5
39	21308.0
40	21308.0
41	23804.0
42	26300.0
43	28279.0
44	30258.0
45	31779.5
46	33301.0
47	33301.0
48	34648.5
49	35996.0
50	37013.0
51	38030.0
52	38786.5
53	39543.0
54	39543.0
55	38351.5
56	37160.0
57	35842.5
58	34525.0
59	33240.5
60	31956.0
61	31956.0
62	29606.5
63	27257.0
64	24572.0
65	21887.0
66	19123.5
67	16360.0
68	16360.0
69	13849.0
70	11338.0
71	9627.5
72	7917.0
73	6435.5
74	4954.0
75	4954.0
76	4038.5
77	3123.0
78	2531.5
79	1940.0
80	1623.0
81	1306.0
82	1306.0
83	1044.5
84	783.0
85	595.5
86	408.0
87	307.0
88	206.0
89	206.0
90	148.0
91	90.0
92	62.0
93	34.0
94	21.5
95	9.0
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	485925.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.38026037299183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32625228671408	68.22762407802777
2	6.37010062006559	9.730998879255328
3	1.6967856337994407	3.888027855202597
4	0.7516124008030877	2.2963340349163732
5	0.41795472946628526	1.5961745530379114
6	0.29118086641597535	1.3344282235491327
7	0.20636938584115957	1.1033783196493527
8	0.15209618077725734	0.9293716711603639
9	0.11329043123733905	0.7787837372108833
>10	0.6554376919418538	8.91875113331583
>50	0.016487231102421066	0.804093357484275
>100	0.0021622594093269115	0.2871614073994503
>500	2.702824261658278E-4	0.10487274979073251
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	508	0.10454288213201626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.115861501260483E-4	0.0
2	0.0	2.0579307506302414E-4	0.0	4.115861501260483E-4	0.0
3	0.0	2.0579307506302414E-4	0.0	4.115861501260483E-4	0.0
4	0.0	2.0579307506302414E-4	0.0	4.115861501260483E-4	0.0
5	0.0	2.0579307506302414E-4	0.0	6.173792251890724E-4	0.0
6	0.0	2.0579307506302414E-4	0.0	6.173792251890724E-4	0.0
7	0.0	2.0579307506302414E-4	0.0	8.231723002520966E-4	0.0
8	0.0	2.0579307506302414E-4	0.0	8.231723002520966E-4	0.0
9	0.0	2.0579307506302414E-4	0.0	0.0014405515254411689	0.0
10	0.0	2.0579307506302414E-4	0.0	0.0020579307506302415	0.0
11	0.0	2.0579307506302414E-4	0.0	0.0022637238256932655	0.0
12	0.0	2.0579307506302414E-4	0.0	0.0026753099758193137	0.0
13	0.0	2.0579307506302414E-4	0.0	0.0026753099758193137	0.0
14	0.0	2.0579307506302414E-4	0.0	0.0026753099758193137	0.0
15	0.0	2.0579307506302414E-4	0.0	0.0026753099758193137	0.0
16	0.0	2.0579307506302414E-4	0.0	0.0028811030508823377	0.0
17	0.0	2.0579307506302414E-4	0.0	0.0028811030508823377	0.0
18	0.0	2.0579307506302414E-4	0.0	0.0030868961259453618	0.0
19	0.0	2.0579307506302414E-4	0.0	0.0032926892010083863	0.0
20	0.0	2.0579307506302414E-4	0.0	0.0034984822760714103	0.0
21	0.0	2.0579307506302414E-4	0.0	0.0037042753511344344	0.0
22	0.0	2.0579307506302414E-4	0.0	0.004321654576323507	0.0
23	0.0	2.0579307506302414E-4	0.0	0.004527447651386531	0.0
24	0.0	2.0579307506302414E-4	0.0	0.005350619951638627	0.0
25	0.0	2.0579307506302414E-4	0.0	0.0065853784020167725	0.0
26	0.0	2.0579307506302414E-4	0.0	0.007820136852394917	0.0
27	0.0	2.0579307506302414E-4	0.0	0.023666203632247775	0.0
28	0.0	2.0579307506302414E-4	0.0	0.07223336934712148	0.0
29	0.0	2.0579307506302414E-4	0.0	0.16278232237485207	0.0
30	0.0	2.0579307506302414E-4	0.0	0.28605237433760355	0.0
31	0.0	2.0579307506302414E-4	0.0	0.6620363224777486	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCGC	40	5.9331825E-5	27.75	6
GGTATCA	180	0.0	25.694445	1
GCGTTAT	45	1.3216767E-4	24.666668	1
GTTATTC	45	1.3216767E-4	24.666668	3
ATAGTAC	55	1.8997298E-5	23.545454	3
CGTTATT	40	0.0019295921	23.125	2
ATGGTCG	40	0.0019295921	23.125	36
CCCTATG	50	2.6989926E-4	22.2	2
TCTAATA	50	2.6989926E-4	22.2	2
ATAGGGG	60	3.7205893E-5	21.583334	3
TCGTTTA	45	0.003822677	20.555557	30
TGACCCG	45	0.003822677	20.555557	13
GTGTTAG	65	6.891657E-5	19.923077	1
CTTAAGG	50	0.0070291813	18.5	3
CCTTAGT	50	0.0070291813	18.5	1
ACACCTC	60	9.2263566E-4	18.5	6
GTCTAGA	50	0.0070291813	18.5	1
GTTATGT	60	9.2263566E-4	18.5	1
ACTGATC	95	3.5977264E-6	17.526316	8
TTATGCT	95	3.5977264E-6	17.526316	4
>>END_MODULE