FastQCFastQC Report
Fri 10 Feb 2017
ERR1632214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117583
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.13670846934949285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.023.887721
CTTATAC20700.017.24879337
GTCTATA1751.3278623E-1015.8571421
TAGACTA1701.4897523E-915.2352945
GTATCAA24300.014.8456791
TAGCGAA750.004106502514.810
TCGCTAA750.004106502514.814
TAAAGCG908.279622E-414.3888884
ACGGACC4250.014.3647068
TCTTATA32500.014.28769237
TATAGGA2151.9826984E-1013.7674422
ACTATCG1102.458475E-413.4545478
TATACAG2355.638867E-1113.38297755
CGTTATT2651.8189894E-1213.2641522
GTTTAAG4050.013.2469141
TACGACA1551.8902756E-613.1290324
TGTACCG3950.013.1139245
TATAGAG1703.7371137E-713.0588245
ACCGTTA850.00940945513.0588248
GTTCTAG2850.012.9824561
Produced by FastQC (version 0.11.5)