##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632213.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814852
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.813047031853635	34.0	31.0	34.0	31.0	34.0
2	32.977335761586154	34.0	33.0	34.0	31.0	34.0
3	32.981627338461465	34.0	33.0	34.0	31.0	34.0
4	36.40676834566277	37.0	37.0	37.0	35.0	37.0
5	36.32550696322768	37.0	37.0	37.0	35.0	37.0
6	36.35697525440202	37.0	37.0	37.0	35.0	37.0
7	36.34479635565722	37.0	37.0	37.0	35.0	37.0
8	36.32903521130218	37.0	37.0	37.0	35.0	37.0
9	38.11856508912048	39.0	39.0	39.0	37.0	39.0
10	38.05907453132593	39.0	38.0	39.0	37.0	39.0
11	38.15497415481585	39.0	39.0	39.0	37.0	39.0
12	38.13243386529088	39.0	38.0	39.0	37.0	39.0
13	38.14927741479434	39.0	39.0	39.0	37.0	39.0
14	39.583040846681364	41.0	39.0	41.0	37.0	41.0
15	39.60815338245473	41.0	39.0	41.0	37.0	41.0
16	39.49626067064939	41.0	39.0	41.0	37.0	41.0
17	39.53730002503522	41.0	39.0	41.0	37.0	41.0
18	39.55078100072161	41.0	39.0	41.0	37.0	41.0
19	39.603351283423244	41.0	39.0	41.0	37.0	41.0
20	39.57218856921257	41.0	39.0	41.0	37.0	41.0
21	39.55122034430792	41.0	39.0	41.0	37.0	41.0
22	39.53210521665284	41.0	39.0	41.0	37.0	41.0
23	39.493641790165576	41.0	39.0	41.0	37.0	41.0
24	39.4408763800052	41.0	39.0	41.0	37.0	41.0
25	39.38146559129756	41.0	39.0	41.0	36.0	41.0
26	39.296945948466714	40.0	39.0	41.0	36.0	41.0
27	39.23808740728378	40.0	39.0	41.0	36.0	41.0
28	39.17687015556199	40.0	39.0	41.0	36.0	41.0
29	39.04066873493591	40.0	39.0	41.0	36.0	41.0
30	39.00213535709552	40.0	39.0	41.0	35.0	41.0
31	38.940126550588325	40.0	39.0	41.0	35.0	41.0
32	38.88878716625841	40.0	38.0	41.0	35.0	41.0
33	38.75210222224404	40.0	38.0	41.0	35.0	41.0
34	38.756820870538455	40.0	38.0	41.0	35.0	41.0
35	38.7403197145003	40.0	38.0	41.0	35.0	41.0
36	38.71013141036654	40.0	38.0	41.0	35.0	41.0
37	38.67775006995135	40.0	38.0	41.0	35.0	41.0
38	38.6193799610236	40.0	38.0	41.0	35.0	41.0
39	38.43910796070943	40.0	38.0	41.0	35.0	41.0
40	38.43993387756304	40.0	38.0	41.0	34.0	41.0
41	38.44667007014771	40.0	38.0	41.0	34.0	41.0
42	38.316213250995276	40.0	38.0	41.0	34.0	41.0
43	37.48962265540246	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	0.0
15	0.0
16	1.0
17	5.0
18	11.0
19	30.0
20	32.0
21	80.0
22	156.0
23	268.0
24	453.0
25	707.0
26	1071.0
27	1622.0
28	2339.0
29	3334.0
30	4639.0
31	6415.0
32	8555.0
33	12224.0
34	17979.0
35	28153.0
36	44946.0
37	82416.0
38	194353.0
39	405059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.192997010500065	17.598533230574386	12.033841728313853	27.174628030611693
2	20.08070177161006	19.403769027995267	32.87922223913054	27.63630696126413
3	21.099046207164	20.887351322694183	28.221689337450233	29.791913132691583
4	16.050767501337663	15.04972682155778	32.18228586295425	36.7172198141503
5	17.23024549243298	33.61457049869179	32.419384133560456	16.73579987531478
6	37.00929739388257	32.245855689131275	14.882702625752897	15.862144291233255
7	30.973354670541397	28.043742912823433	20.2574209795153	20.725481437119868
8	28.14646095241835	31.55444178820203	19.043703641888342	21.255393617491276
9	27.00699022644603	13.74985887007702	18.96577538006902	40.27737552340793
10	18.400273914772253	25.134134787666962	31.996976137998068	24.468615159562717
11	37.96934903516222	20.265152444861155	20.080088163249275	21.685410356727356
12	23.144816482011457	23.654847751493524	27.68625468183179	25.514081084663225
13	32.19480347351421	18.459057595735175	22.487887370958163	26.85825155979245
14	24.182673663438266	19.329522416340637	23.19415059421834	33.29365332600276
15	26.95839244427209	25.756579108844306	20.553793817773045	26.731234629110563
16	26.849906486085818	24.16242458753246	22.44235763058813	26.545311295793596
17	25.48892314187116	24.65797469970007	23.80726806831179	26.045834090116976
18	25.85647454997963	23.74504818052849	24.334971258584382	26.0635060109075
19	26.469592024073087	23.70528635874981	24.265878957160318	25.55924266001679
20	26.48407318138754	23.67853303421971	24.076642138695124	25.76075164569762
21	26.714667203369448	23.573483282853818	23.592382420365908	26.119467093410826
22	27.00416762798643	23.50120021795369	23.461192952830697	26.03343920122918
23	26.548747502613974	23.45972029276482	23.537771276256304	26.453760928364904
24	26.31177195367993	23.844452734975185	23.330739815328428	26.513035496016457
25	26.667173916244913	23.29551869541954	23.53985754468296	26.49744984365259
26	26.283791412428272	23.768488019910365	23.673256002316986	26.27446456534438
27	26.149165738072682	23.523412840614	23.79536406611262	26.5320573552007
28	26.06006980408712	23.524640057335567	23.978587522642147	26.436702615935165
29	25.987295852498367	23.7405074786587	24.13702120139608	26.135175467446846
30	25.825303245251895	23.906549901086333	23.992455071595824	26.275691782065948
31	26.386509452023187	23.5186266953999	23.78370550725776	26.311158345319154
32	25.64441150049334	23.659633896707625	23.96545630372141	26.730498299077627
33	25.596059161663714	23.41590865580498	24.39314133118652	26.594890851344783
34	26.129898435544124	22.744989274125853	24.386268917545763	26.73884337278426
35	25.61127664901111	22.910908974881327	24.97435117051931	26.50346320558825
36	25.476528252983364	22.765238350031662	24.924526171623803	26.83370722536117
37	25.208381399321595	21.8612705129275	25.868501273850956	27.061846813899948
38	24.63662112874485	21.901277778050492	26.833339060344702	26.628762032859953
39	24.480396440089734	21.63251731602794	27.277714235223083	26.609372008659243
40	24.046329885672492	21.47261097720813	27.939061326474008	26.54199781064537
41	23.37663772071493	21.669333817674865	28.29986304261387	26.654165418996335
42	22.993009773553972	21.93563984625429	28.464432804975626	26.60691757521611
43	22.606559227933413	21.673629076200342	28.72251648152057	26.997295214345673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	39.0
2	47.0
3	83.0
4	119.0
5	119.0
6	177.5
7	236.0
8	211.0
9	186.0
10	286.5
11	387.0
12	387.0
13	669.5
14	952.0
15	1333.0
16	1714.0
17	1779.5
18	1845.0
19	1845.0
20	2329.5
21	2814.0
22	3030.5
23	3247.0
24	4099.5
25	4952.0
26	4952.0
27	6134.0
28	7316.0
29	9046.0
30	10776.0
31	12981.5
32	15187.0
33	15187.0
34	18163.5
35	21140.0
36	24134.5
37	27129.0
38	30333.5
39	33538.0
40	33538.0
41	37098.5
42	40659.0
43	43852.0
44	47045.0
45	49841.0
46	52637.0
47	52637.0
48	55036.0
49	57435.0
50	59323.5
51	61212.0
52	62211.5
53	63211.0
54	63211.0
55	63082.5
56	62954.0
57	61422.0
58	59890.0
59	57821.0
60	55752.0
61	55752.0
62	51939.5
63	48127.0
64	43826.5
65	39526.0
66	34937.0
67	30348.0
68	30348.0
69	26358.5
70	22369.0
71	19142.0
72	15915.0
73	12993.0
74	10071.0
75	10071.0
76	8258.0
77	6445.0
78	5240.5
79	4036.0
80	3353.0
81	2670.0
82	2670.0
83	2056.5
84	1443.0
85	1127.5
86	812.0
87	604.5
88	397.0
89	397.0
90	301.0
91	205.0
92	129.0
93	53.0
94	36.5
95	20.0
96	20.0
97	11.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	814852.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.98311607444603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.01028572724086	68.5228915795979
2	6.761986874477395	10.411176409035477
3	1.7437328405102352	4.027139629914688
4	0.8161188474627064	2.513094878590585
5	0.42537304538340004	1.6373271263845444
6	0.25771156140306584	1.1903663427131366
7	0.18038928364971124	0.9720850413254323
8	0.12662793221083704	0.7798570242923162
9	0.10099715160044581	0.6997567900360988
>10	0.5488364226669229	7.419595701668991
>50	0.023949508358031373	1.2098024826312503
>100	0.0036715406524937015	0.4431407662781743
>500	3.192643838324672E-4	0.17376622753140997
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.2272167215641614E-4
9	0.0	0.0	0.0	1.2272167215641614E-4	1.2272167215641614E-4
10	0.0	0.0	0.0	1.2272167215641614E-4	1.2272167215641614E-4
11	0.0	0.0	0.0	1.2272167215641614E-4	1.2272167215641614E-4
12	0.0	0.0	0.0	1.2272167215641614E-4	1.2272167215641614E-4
13	0.0	0.0	0.0	1.2272167215641614E-4	1.2272167215641614E-4
14	0.0	0.0	0.0	1.2272167215641614E-4	1.2272167215641614E-4
15	0.0	0.0	0.0	1.2272167215641614E-4	1.2272167215641614E-4
16	0.0	0.0	0.0	3.681650164692484E-4	2.454433443128323E-4
17	0.0	0.0	0.0	6.136083607820807E-4	2.454433443128323E-4
18	0.0	0.0	0.0	6.136083607820807E-4	2.454433443128323E-4
19	0.0	0.0	0.0	7.363300329384968E-4	2.454433443128323E-4
20	0.0	0.0	0.0	8.590517050949129E-4	2.454433443128323E-4
21	0.0	0.0	0.0	0.0011044950494077453	2.454433443128323E-4
22	0.0	0.0	0.0	0.0015953817380334097	2.454433443128323E-4
23	0.0	0.0	0.0	0.0035589284925360678	2.454433443128323E-4
24	0.0	0.0	0.0	0.006013361935664391	2.454433443128323E-4
25	0.0	0.0	0.0	0.009449568756044042	2.454433443128323E-4
26	0.0	0.0	0.0	0.01656742574111618	2.454433443128323E-4
27	0.0	0.0	0.0	0.046020627058656054	2.454433443128323E-4
28	0.0	0.0	0.0	0.1277532607148292	2.454433443128323E-4
29	0.0	0.0	0.0	0.24151625080382697	2.454433443128323E-4
30	0.0	0.0	0.0	0.3901321957852469	2.454433443128323E-4
31	0.0	0.0	0.0	0.7846823717681247	2.454433443128323E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGGC	40	0.0019307074	23.125	3
CATTACG	40	0.0019307074	23.125	4
GGTATCA	370	0.0	23.0	1
GTTCTAA	45	0.003824867	20.555555	1
ATTACGA	45	0.003824867	20.555555	5
ATTCGCC	50	0.0070331674	18.5	11
CGATTCG	50	0.0070331674	18.5	9
TGCGCGG	80	1.6157295E-5	18.5	10
TACGATT	50	0.0070331674	18.5	7
TAATAGT	85	2.721541E-5	17.411764	4
CATATCG	65	0.0015794009	17.076923	9
TTTATAC	65	0.0015794009	17.076923	3
TCTTATA	1370	0.0	16.60949	37
TATTATA	80	3.381054E-4	16.1875	33
TTACTCG	85	5.361772E-4	15.235294	9
TGGTCGA	85	5.361772E-4	15.235294	10
CTTATAC	735	0.0	14.85034	37
TTAGGCA	75	0.004103042	14.8	4
GGCTATA	75	0.004103042	14.8	1
AGACGTC	90	8.270372E-4	14.388888	14
>>END_MODULE