FastQCFastQC Report
Fri 10 Feb 2017
ERR1632212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8373688
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165080.1971413312748218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143710.17162091541982458No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA120450.1438434295617415No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC119110.14224317887172297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115930.13844556902526103No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG108320.1293575781662751No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG88320.10547323950928195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.023.515691
CTTATAC38750.016.70967737
GTATCAA95800.015.2557412
TCTTATA64400.015.05279537
ATCTCGC40400.014.69925711
CGGCCTT40150.014.65255424
TCGTTTA5250.014.447618530
CGAATTA4700.014.17021415
TTCCTCG43350.013.95501719
TCTCGCC42300.013.82033212
GTATTAA15500.013.7258071
TAGGACA34550.013.5470324
GGCCTTT45250.013.24640825
TCCTCGG46650.013.007503520
GCTTAGG32500.012.9215381
TATACTG5500.012.7818175
CTTAGGA34200.012.7119882
TAGAACA34050.012.6593254
AATCTCG47300.012.5940810
AAATCTC46850.012.4386349
Produced by FastQC (version 0.11.5)