FastQCFastQC Report
Fri 10 Feb 2017
ERR1632211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892229
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.10479704095011756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19730.10426856368864444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.023.7330681
GTATCAA17750.016.7802832
CTTATAC13850.015.89530737
ATACCGT2055.456968E-1215.3414646
TCTTATA23400.015.02136837
TTAGACT1252.9610092E-614.7999994
TATATCG750.00410571114.7999995
ATAAGAC1252.9610092E-614.7999993
GTCTAAT1152.2112263E-514.4782611
ATATCGC908.2774984E-414.3888896
GTAGCAC2252.7284841E-1113.9777773
TACCGTC2151.9826984E-1013.7674427
TGTAATA1501.3066601E-613.5666662
GGACCGT1254.8476177E-513.326
CTCTTAT36300.013.30165337
TACTCGA850.00940766813.0588232
TATACTG1000.001833241212.9500015
GTACTAT2305.7661964E-1012.8695651
ACCGTCG2305.7661964E-1012.8695658
CGAATCG1050.002644657612.33333316
Produced by FastQC (version 0.11.5)